Adaptation of O157:H7 and non-O157 Escherichia coli strains in orange juice and subsequent resistance to UV-C radiation
This study assessed the acid-adaptation of pathogenic and non-pathogenic strains of Escherichia coli in orange juice and the microbial resistance to the subsequent UV-C radiation treatment. Nine Shiga toxin-producing E. coli (STEC) and one strain of a non-pathogenic surrogate E. coli were used in th...
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| Formato: | Articulo |
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2022
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/159801 |
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I19-R120-10915-1598012023-11-08T20:07:39Z http://sedici.unlp.edu.ar/handle/10915/159801 Adaptation of O157:H7 and non-O157 Escherichia coli strains in orange juice and subsequent resistance to UV-C radiation Oteiza, Juan Martín Caturla, Magdevis Y.R. Do Prado-Silva, Leonardo Cámara Jr., Antonio A. Barril, Patricia A. Sant’Ana, Anderson S. Giannuzzi, Leda Zaritzky, Noemí Elisabet 2022 2023-11-06T13:05:31Z en Química Foodborne pathogens Fruit juices Emerging technologies Acid adaptation Irradiation This study assessed the acid-adaptation of pathogenic and non-pathogenic strains of Escherichia coli in orange juice and the microbial resistance to the subsequent UV-C radiation treatment. Nine Shiga toxin-producing E. coli (STEC) and one strain of a non-pathogenic surrogate E. coli were used in this study. Each E. coli strain was inoculated in orange juice, following pre-exposure during 0, 1, 2, and 3 h at 10 ◦C. Then, the inoculated juices with the ten different strains separately were exposed to 0 and 2 J/cm2 of UV-C radiation. The D value (i.e., the UV-C dose in J/cm2 required to cause a one-log reduction in the target microorganism) was calculated. Further, the resistance coefficient [RC; i.e., the ratio between the D-values for the control condition (D0h) and each preexposure tested time (D1h, D2h, D3h)] were determined. The results indicated that the resistance of E. coli was influenced by the pre-exposure period in the orange juice, with increased resistance to UV-C observed for periods >2 h. Furthermore, the sensitivity of cells to subsequent UV-C treatment was found to be strain-dependent. The results may allow the development of more reliable UV-C radiation processes for orange juice processing aiming the inactivation of pathogenic E. coli. Centro de Investigación y Desarrollo en Criotecnología de Alimentos Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Química Foodborne pathogens Fruit juices Emerging technologies Acid adaptation Irradiation |
| spellingShingle |
Química Foodborne pathogens Fruit juices Emerging technologies Acid adaptation Irradiation Oteiza, Juan Martín Caturla, Magdevis Y.R. Do Prado-Silva, Leonardo Cámara Jr., Antonio A. Barril, Patricia A. Sant’Ana, Anderson S. Giannuzzi, Leda Zaritzky, Noemí Elisabet Adaptation of O157:H7 and non-O157 Escherichia coli strains in orange juice and subsequent resistance to UV-C radiation |
| topic_facet |
Química Foodborne pathogens Fruit juices Emerging technologies Acid adaptation Irradiation |
| description |
This study assessed the acid-adaptation of pathogenic and non-pathogenic strains of Escherichia coli in orange juice and the microbial resistance to the subsequent UV-C radiation treatment. Nine Shiga toxin-producing E. coli (STEC) and one strain of a non-pathogenic surrogate E. coli were used in this study. Each E. coli strain was inoculated in orange juice, following pre-exposure during 0, 1, 2, and 3 h at 10 ◦C. Then, the inoculated juices with the ten different strains separately were exposed to 0 and 2 J/cm2 of UV-C radiation. The D value (i.e., the UV-C dose in J/cm2 required to cause a one-log reduction in the target microorganism) was calculated. Further, the resistance coefficient [RC; i.e., the ratio between the D-values for the control condition (D0h) and each preexposure tested time (D1h, D2h, D3h)] were determined. The results indicated that the resistance of E. coli was influenced by the pre-exposure period in the orange juice, with increased resistance to UV-C observed for periods >2 h. Furthermore, the sensitivity of cells to subsequent UV-C treatment was found to be strain-dependent. The results may allow the development of more reliable UV-C radiation processes for orange juice processing aiming the inactivation of pathogenic E. coli. |
| format |
Articulo Articulo |
| author |
Oteiza, Juan Martín Caturla, Magdevis Y.R. Do Prado-Silva, Leonardo Cámara Jr., Antonio A. Barril, Patricia A. Sant’Ana, Anderson S. Giannuzzi, Leda Zaritzky, Noemí Elisabet |
| author_facet |
Oteiza, Juan Martín Caturla, Magdevis Y.R. Do Prado-Silva, Leonardo Cámara Jr., Antonio A. Barril, Patricia A. Sant’Ana, Anderson S. Giannuzzi, Leda Zaritzky, Noemí Elisabet |
| author_sort |
Oteiza, Juan Martín |
| title |
Adaptation of O157:H7 and non-O157 Escherichia coli strains in orange juice and subsequent resistance to UV-C radiation |
| title_short |
Adaptation of O157:H7 and non-O157 Escherichia coli strains in orange juice and subsequent resistance to UV-C radiation |
| title_full |
Adaptation of O157:H7 and non-O157 Escherichia coli strains in orange juice and subsequent resistance to UV-C radiation |
| title_fullStr |
Adaptation of O157:H7 and non-O157 Escherichia coli strains in orange juice and subsequent resistance to UV-C radiation |
| title_full_unstemmed |
Adaptation of O157:H7 and non-O157 Escherichia coli strains in orange juice and subsequent resistance to UV-C radiation |
| title_sort |
adaptation of o157:h7 and non-o157 escherichia coli strains in orange juice and subsequent resistance to uv-c radiation |
| publishDate |
2022 |
| url |
http://sedici.unlp.edu.ar/handle/10915/159801 |
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