Edible coating technology for the stabilization of functional foods based on pumpkin

Vegetable foods were prepared from blanched Cucurbita moschata Duchesne ex Poiret (pumpkin) and enriched with probiotics, particularly Lactobacillus casei (ATTC 393) or enriched with probiotics and fortified with iron (Fe). With the purpose of extending the shelf life of the product, the application...

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Autores principales: Flores, S., de Escalada Pla, M., Miletti, A., Genevois, C.
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_97815361_v_n_p209_Flores
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spelling todo:paper_97815361_v_n_p209_Flores2023-10-03T16:43:50Z Edible coating technology for the stabilization of functional foods based on pumpkin Flores, S. de Escalada Pla, M. Miletti, A. Genevois, C. Biopolymers Edible coatings Food stabilization Iron Probiotics Vegetable foods were prepared from blanched Cucurbita moschata Duchesne ex Poiret (pumpkin) and enriched with probiotics, particularly Lactobacillus casei (ATTC 393) or enriched with probiotics and fortified with iron (Fe). With the purpose of extending the shelf life of the product, the application of edible coatings (EC) was tested using both a drying and a non-drying process. Likewise, the probiotic survival capacity to the different applied technologies was studied and the effect of processes used on the color characteristics of the final product was evaluated. According to the results, it was verified that the application of an EC based on Agar-agar extended up to 14 days (8°C) the shelf life of the pumpkin impregnated with L. casei. The pH reduction and drying processes contributed to prolonging the storage time, reaching 18 days at 20 °C with a viability of the probiotics around 108 CFU g-1 product. In addition, a loss of color was observed, possibly as a result of the pigment degradation. It was also demonstrated that the pumpkin tissue was able to contain Lactobacillus casei. In the case of combined enrichment with probiotics and Fe, the blanched pumpkin was subjected to a dry infusion process to incorporate the mineral and, in addition, it was coated with an EC based on hydroxypropyl methylcellulose (HPMC) containing L. casei. Two kinds of HPMC based coatings were evaluated, one with 28-30% of methoxyl groups (E4M) and the other one with 19-24% of methoxyl groups (K4M). Six coating systems were studied: A: 3% w/v HPMC E4M, B1 and B2: 1.6% w/v HPMC E4M, C: 3% w/v HPMC K4M, D1 and D2: 1.6% w/v HPMC K4M. Systems A, B1, C and D1 were submitted to a drying process with forced air convection at 46 °C for 4 h while systems B2 and D2 were dehydrated at 38 °C for 20 h. The viability of Lactobacillus casei in the final product during storage and the bioaccessibility of Fe after in vitro gastrointestinal digestion were evaluated. The initial count of L. casei and the content of bioaccessible Fe in the intestinal lumen (55-70%) did not show significant differences among the studied systems, consequently, the type of hydrocolloid and drying process did not affect these parameters. However, at 14 days of storage at 8 °C, a lower stabilization capacity of L. casei was observed for the systems D1 and D2. The obtained results allow confirming that EC is a realistic alternative for the stabilization of vegetal foods enriched with Fe and / or probiotics. The EC technology has proved to be useful for adding value to a vegetal product and to expand the offer of functional foods. © 2018 Nova Science Publishers, Inc. CHAP info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_97815361_v_n_p209_Flores
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Biopolymers
Edible coatings
Food stabilization
Iron
Probiotics
spellingShingle Biopolymers
Edible coatings
Food stabilization
Iron
Probiotics
Flores, S.
de Escalada Pla, M.
Miletti, A.
Genevois, C.
Edible coating technology for the stabilization of functional foods based on pumpkin
topic_facet Biopolymers
Edible coatings
Food stabilization
Iron
Probiotics
description Vegetable foods were prepared from blanched Cucurbita moschata Duchesne ex Poiret (pumpkin) and enriched with probiotics, particularly Lactobacillus casei (ATTC 393) or enriched with probiotics and fortified with iron (Fe). With the purpose of extending the shelf life of the product, the application of edible coatings (EC) was tested using both a drying and a non-drying process. Likewise, the probiotic survival capacity to the different applied technologies was studied and the effect of processes used on the color characteristics of the final product was evaluated. According to the results, it was verified that the application of an EC based on Agar-agar extended up to 14 days (8°C) the shelf life of the pumpkin impregnated with L. casei. The pH reduction and drying processes contributed to prolonging the storage time, reaching 18 days at 20 °C with a viability of the probiotics around 108 CFU g-1 product. In addition, a loss of color was observed, possibly as a result of the pigment degradation. It was also demonstrated that the pumpkin tissue was able to contain Lactobacillus casei. In the case of combined enrichment with probiotics and Fe, the blanched pumpkin was subjected to a dry infusion process to incorporate the mineral and, in addition, it was coated with an EC based on hydroxypropyl methylcellulose (HPMC) containing L. casei. Two kinds of HPMC based coatings were evaluated, one with 28-30% of methoxyl groups (E4M) and the other one with 19-24% of methoxyl groups (K4M). Six coating systems were studied: A: 3% w/v HPMC E4M, B1 and B2: 1.6% w/v HPMC E4M, C: 3% w/v HPMC K4M, D1 and D2: 1.6% w/v HPMC K4M. Systems A, B1, C and D1 were submitted to a drying process with forced air convection at 46 °C for 4 h while systems B2 and D2 were dehydrated at 38 °C for 20 h. The viability of Lactobacillus casei in the final product during storage and the bioaccessibility of Fe after in vitro gastrointestinal digestion were evaluated. The initial count of L. casei and the content of bioaccessible Fe in the intestinal lumen (55-70%) did not show significant differences among the studied systems, consequently, the type of hydrocolloid and drying process did not affect these parameters. However, at 14 days of storage at 8 °C, a lower stabilization capacity of L. casei was observed for the systems D1 and D2. The obtained results allow confirming that EC is a realistic alternative for the stabilization of vegetal foods enriched with Fe and / or probiotics. The EC technology has proved to be useful for adding value to a vegetal product and to expand the offer of functional foods. © 2018 Nova Science Publishers, Inc.
format CHAP
author Flores, S.
de Escalada Pla, M.
Miletti, A.
Genevois, C.
author_facet Flores, S.
de Escalada Pla, M.
Miletti, A.
Genevois, C.
author_sort Flores, S.
title Edible coating technology for the stabilization of functional foods based on pumpkin
title_short Edible coating technology for the stabilization of functional foods based on pumpkin
title_full Edible coating technology for the stabilization of functional foods based on pumpkin
title_fullStr Edible coating technology for the stabilization of functional foods based on pumpkin
title_full_unstemmed Edible coating technology for the stabilization of functional foods based on pumpkin
title_sort edible coating technology for the stabilization of functional foods based on pumpkin
url http://hdl.handle.net/20.500.12110/paper_97815361_v_n_p209_Flores
work_keys_str_mv AT floress ediblecoatingtechnologyforthestabilizationoffunctionalfoodsbasedonpumpkin
AT deescaladaplam ediblecoatingtechnologyforthestabilizationoffunctionalfoodsbasedonpumpkin
AT milettia ediblecoatingtechnologyforthestabilizationoffunctionalfoodsbasedonpumpkin
AT genevoisc ediblecoatingtechnologyforthestabilizationoffunctionalfoodsbasedonpumpkin
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