Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus
The action of protease and hemicellulase for the extraction of fractions enriched in soluble fiber from bracts and stems of Cynara cardunculus was evaluated. Using a two-factor simplex design comprising protease amounts of 0–200 μL and hemicellulase amounts of 0–200 mg for 5 g of material, we explor...
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todo:paper_16616596_v16_n3_p6057_Domingo2023-10-03T16:28:40Z Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus Domingo, C.S. Soria, M. Rojas, A.M. Fissore, E.N. Gerschenson, L.N. Biomass to chemicals Cynara cardunculus Hemicellulase Inulin Pectin Polyphenols Protease galacturonic acid hemicellulose inulin polyphenol protein proteinase uronic acid glycosidase hemicellulase peptide hydrolase plant extract Article cardoon chemical composition controlled study dietary fiber electrospray mass spectrometry enzyme activity enzyme isolation flow rate high performance liquid chromatography hydrolysis nonhuman protein analysis spectrophotometry chemistry Cynara dietary fiber metabolism Cynara cardunculus Cynara Dietary Fiber Glycoside Hydrolases Peptide Hydrolases Plant Extracts The action of protease and hemicellulase for the extraction of fractions enriched in soluble fiber from bracts and stems of Cynara cardunculus was evaluated. Using a two-factor simplex design comprising protease amounts of 0–200 μL and hemicellulase amounts of 0–200 mg for 5 g of material, we explored the effect of a 5 h enzymatic treatment at 40 °C on the chemical composition and yield of the fractions isolated. The fractions contained inulin and pectin. In general, the protein, inulin, and polyphenol contents and also the yields were higher for fractions obtained from stems. The most marked effects were observed when enzymes were used at higher concentrations, especially for hemicellulase. The inclusion of a pre-heating step increased the yield and the inulin content for fractions isolated from bracts and stems and decreased the protein and polyphenol contents, and the galacturonic acid for bracts. These fractions, in general, contained the polyphenolic compounds monocaffeoylquinic acid, apigenin, and pinoresinol. © 2015 by the authors; licensee MDPI, Basel, Switzerland. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_16616596_v16_n3_p6057_Domingo |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biomass to chemicals Cynara cardunculus Hemicellulase Inulin Pectin Polyphenols Protease galacturonic acid hemicellulose inulin polyphenol protein proteinase uronic acid glycosidase hemicellulase peptide hydrolase plant extract Article cardoon chemical composition controlled study dietary fiber electrospray mass spectrometry enzyme activity enzyme isolation flow rate high performance liquid chromatography hydrolysis nonhuman protein analysis spectrophotometry chemistry Cynara dietary fiber metabolism Cynara cardunculus Cynara Dietary Fiber Glycoside Hydrolases Peptide Hydrolases Plant Extracts |
| spellingShingle |
Biomass to chemicals Cynara cardunculus Hemicellulase Inulin Pectin Polyphenols Protease galacturonic acid hemicellulose inulin polyphenol protein proteinase uronic acid glycosidase hemicellulase peptide hydrolase plant extract Article cardoon chemical composition controlled study dietary fiber electrospray mass spectrometry enzyme activity enzyme isolation flow rate high performance liquid chromatography hydrolysis nonhuman protein analysis spectrophotometry chemistry Cynara dietary fiber metabolism Cynara cardunculus Cynara Dietary Fiber Glycoside Hydrolases Peptide Hydrolases Plant Extracts Domingo, C.S. Soria, M. Rojas, A.M. Fissore, E.N. Gerschenson, L.N. Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus |
| topic_facet |
Biomass to chemicals Cynara cardunculus Hemicellulase Inulin Pectin Polyphenols Protease galacturonic acid hemicellulose inulin polyphenol protein proteinase uronic acid glycosidase hemicellulase peptide hydrolase plant extract Article cardoon chemical composition controlled study dietary fiber electrospray mass spectrometry enzyme activity enzyme isolation flow rate high performance liquid chromatography hydrolysis nonhuman protein analysis spectrophotometry chemistry Cynara dietary fiber metabolism Cynara cardunculus Cynara Dietary Fiber Glycoside Hydrolases Peptide Hydrolases Plant Extracts |
| description |
The action of protease and hemicellulase for the extraction of fractions enriched in soluble fiber from bracts and stems of Cynara cardunculus was evaluated. Using a two-factor simplex design comprising protease amounts of 0–200 μL and hemicellulase amounts of 0–200 mg for 5 g of material, we explored the effect of a 5 h enzymatic treatment at 40 °C on the chemical composition and yield of the fractions isolated. The fractions contained inulin and pectin. In general, the protein, inulin, and polyphenol contents and also the yields were higher for fractions obtained from stems. The most marked effects were observed when enzymes were used at higher concentrations, especially for hemicellulase. The inclusion of a pre-heating step increased the yield and the inulin content for fractions isolated from bracts and stems and decreased the protein and polyphenol contents, and the galacturonic acid for bracts. These fractions, in general, contained the polyphenolic compounds monocaffeoylquinic acid, apigenin, and pinoresinol. © 2015 by the authors; licensee MDPI, Basel, Switzerland. |
| format |
JOUR |
| author |
Domingo, C.S. Soria, M. Rojas, A.M. Fissore, E.N. Gerschenson, L.N. |
| author_facet |
Domingo, C.S. Soria, M. Rojas, A.M. Fissore, E.N. Gerschenson, L.N. |
| author_sort |
Domingo, C.S. |
| title |
Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus |
| title_short |
Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus |
| title_full |
Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus |
| title_fullStr |
Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus |
| title_full_unstemmed |
Protease and hemicellulase assisted extraction of dietary fiber from wastes of Cynara cardunculus |
| title_sort |
protease and hemicellulase assisted extraction of dietary fiber from wastes of cynara cardunculus |
| url |
http://hdl.handle.net/20.500.12110/paper_16616596_v16_n3_p6057_Domingo |
| work_keys_str_mv |
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| _version_ |
1807323965870309376 |