A computational investigation of the reactions of tyrosyl, tryptophanyl, and cysteinyl radicals with nitric oxide and molecular oxygen
Proteins are main targets of oxidants in biological systems. This oxidation may occur in the protein backbone as well as in certain amino acid side chains, depending on the oxidant and amino acid intrinsic reactivity. Moreover, many enzymes are capable of generating stable amino acid radicals, such...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_10715762_v53_n1_p18_Pedron |
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todo:paper_10715762_v53_n1_p18_Pedron2023-10-03T16:02:39Z A computational investigation of the reactions of tyrosyl, tryptophanyl, and cysteinyl radicals with nitric oxide and molecular oxygen Pedron, F.N. Bartesaghi, S. Estrin, D.A. Radi, R. Zeida, A. Amino acid-derived radicals nitric oxide one-electron oxidation oxygen cysteinyl radical nitric oxide oxidizing agent oxygen radical tryptophanyl radical tyrosyl radical unclassified drug Article diffusion energy transfer mathematical computing oxidation quantum mechanics rate constant reaction analysis Proteins are main targets of oxidants in biological systems. This oxidation may occur in the protein backbone as well as in certain amino acid side chains, depending on the oxidant and amino acid intrinsic reactivity. Moreover, many enzymes are capable of generating stable amino acid radicals, such as tyrosyl, tryptophanyl and cysteinyl radicals. These species react very rapidly (many times as diffusion-controlled reactions) with relevant cellular open-shell species such as nitric oxide (·NO) or molecular oxygen (O 2 ). The exception to this apparent rule is tyrosyl radical, that reacts at diffusion rates with ·NO, but shows very slow reactivity towards O 2 (rate constant <10 3 M −1 s −1 ). In this work, we provide a comparative molecular-level description of the reaction mechanisms involved in the reactions of tyrosyl, tryptophanyl and cysteinyl radicals towards ·NO and O 2 , through quantum mechanics simulations which allow us to obtain relevant energetic and structural parameters, proposing a molecular explanation to this tyrosyl discrimination capability, namely, its marginal reactivity with O 2 . © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10715762_v53_n1_p18_Pedron |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Amino acid-derived radicals nitric oxide one-electron oxidation oxygen cysteinyl radical nitric oxide oxidizing agent oxygen radical tryptophanyl radical tyrosyl radical unclassified drug Article diffusion energy transfer mathematical computing oxidation quantum mechanics rate constant reaction analysis |
| spellingShingle |
Amino acid-derived radicals nitric oxide one-electron oxidation oxygen cysteinyl radical nitric oxide oxidizing agent oxygen radical tryptophanyl radical tyrosyl radical unclassified drug Article diffusion energy transfer mathematical computing oxidation quantum mechanics rate constant reaction analysis Pedron, F.N. Bartesaghi, S. Estrin, D.A. Radi, R. Zeida, A. A computational investigation of the reactions of tyrosyl, tryptophanyl, and cysteinyl radicals with nitric oxide and molecular oxygen |
| topic_facet |
Amino acid-derived radicals nitric oxide one-electron oxidation oxygen cysteinyl radical nitric oxide oxidizing agent oxygen radical tryptophanyl radical tyrosyl radical unclassified drug Article diffusion energy transfer mathematical computing oxidation quantum mechanics rate constant reaction analysis |
| description |
Proteins are main targets of oxidants in biological systems. This oxidation may occur in the protein backbone as well as in certain amino acid side chains, depending on the oxidant and amino acid intrinsic reactivity. Moreover, many enzymes are capable of generating stable amino acid radicals, such as tyrosyl, tryptophanyl and cysteinyl radicals. These species react very rapidly (many times as diffusion-controlled reactions) with relevant cellular open-shell species such as nitric oxide (·NO) or molecular oxygen (O 2 ). The exception to this apparent rule is tyrosyl radical, that reacts at diffusion rates with ·NO, but shows very slow reactivity towards O 2 (rate constant <10 3 M −1 s −1 ). In this work, we provide a comparative molecular-level description of the reaction mechanisms involved in the reactions of tyrosyl, tryptophanyl and cysteinyl radicals towards ·NO and O 2 , through quantum mechanics simulations which allow us to obtain relevant energetic and structural parameters, proposing a molecular explanation to this tyrosyl discrimination capability, namely, its marginal reactivity with O 2 . © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group. |
| format |
JOUR |
| author |
Pedron, F.N. Bartesaghi, S. Estrin, D.A. Radi, R. Zeida, A. |
| author_facet |
Pedron, F.N. Bartesaghi, S. Estrin, D.A. Radi, R. Zeida, A. |
| author_sort |
Pedron, F.N. |
| title |
A computational investigation of the reactions of tyrosyl, tryptophanyl, and cysteinyl radicals with nitric oxide and molecular oxygen |
| title_short |
A computational investigation of the reactions of tyrosyl, tryptophanyl, and cysteinyl radicals with nitric oxide and molecular oxygen |
| title_full |
A computational investigation of the reactions of tyrosyl, tryptophanyl, and cysteinyl radicals with nitric oxide and molecular oxygen |
| title_fullStr |
A computational investigation of the reactions of tyrosyl, tryptophanyl, and cysteinyl radicals with nitric oxide and molecular oxygen |
| title_full_unstemmed |
A computational investigation of the reactions of tyrosyl, tryptophanyl, and cysteinyl radicals with nitric oxide and molecular oxygen |
| title_sort |
computational investigation of the reactions of tyrosyl, tryptophanyl, and cysteinyl radicals with nitric oxide and molecular oxygen |
| url |
http://hdl.handle.net/20.500.12110/paper_10715762_v53_n1_p18_Pedron |
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