Characterization of a novel cysteine peptidase from tissue culture of garlic (Allium sativum L.)

A simple and efficient medium for callus tissue culture from garlic to obtain maximal proteolytic activity is described. Murashige and Skoog basal medium supplemented with 4.44 μM naphthaleneacetic acid (NAA) and 0.54 μM benzyladenine (BA) resulted in the best biomass production and protease express...

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Autores principales: Parisi, M., Moreno, S., Fernández, C.
Formato: JOUR
Materias:
Callus culture
Cysteine protease
Endopeptidase
In vitro culture
Protease modulators
Allium sativum
Sativum
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_10545476_v38_n6_p608_Parisi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_10545476_v38_n6_p608_Parisi2023-10-03T16:00:44Z Characterization of a novel cysteine peptidase from tissue culture of garlic (Allium sativum L.) Parisi, M. Moreno, S. Fernández, C. Callus culture Cysteine protease Endopeptidase In vitro culture Protease modulators Allium sativum Sativum A simple and efficient medium for callus tissue culture from garlic to obtain maximal proteolytic activity is described. Murashige and Skoog basal medium supplemented with 4.44 μM naphthaleneacetic acid (NAA) and 0.54 μM benzyladenine (BA) resulted in the best biomass production and protease expression. The protease activity belongs to the class of cysteine proteases since they are inhibited by E64 and Leupeptin and also they are activated by 2-mercaptoethanol and cysteine. They showed good thermal stability. Three active protease bands were found in zymograms of Allium sativum. The in vitro system revealed a significantly higher protease level than storage and embryo tissues of in vivo bulbs. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_10545476_v38_n6_p608_Parisi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Callus culture
Cysteine protease
Endopeptidase
In vitro culture
Protease modulators
Allium sativum
Sativum
spellingShingle Callus culture
Cysteine protease
Endopeptidase
In vitro culture
Protease modulators
Allium sativum
Sativum
Parisi, M.
Moreno, S.
Fernández, C.
Characterization of a novel cysteine peptidase from tissue culture of garlic (Allium sativum L.)
topic_facet Callus culture
Cysteine protease
Endopeptidase
In vitro culture
Protease modulators
Allium sativum
Sativum
description A simple and efficient medium for callus tissue culture from garlic to obtain maximal proteolytic activity is described. Murashige and Skoog basal medium supplemented with 4.44 μM naphthaleneacetic acid (NAA) and 0.54 μM benzyladenine (BA) resulted in the best biomass production and protease expression. The protease activity belongs to the class of cysteine proteases since they are inhibited by E64 and Leupeptin and also they are activated by 2-mercaptoethanol and cysteine. They showed good thermal stability. Three active protease bands were found in zymograms of Allium sativum. The in vitro system revealed a significantly higher protease level than storage and embryo tissues of in vivo bulbs.
format JOUR
author Parisi, M.
Moreno, S.
Fernández, C.
author_facet Parisi, M.
Moreno, S.
Fernández, C.
author_sort Parisi, M.
title Characterization of a novel cysteine peptidase from tissue culture of garlic (Allium sativum L.)
title_short Characterization of a novel cysteine peptidase from tissue culture of garlic (Allium sativum L.)
title_full Characterization of a novel cysteine peptidase from tissue culture of garlic (Allium sativum L.)
title_fullStr Characterization of a novel cysteine peptidase from tissue culture of garlic (Allium sativum L.)
title_full_unstemmed Characterization of a novel cysteine peptidase from tissue culture of garlic (Allium sativum L.)
title_sort characterization of a novel cysteine peptidase from tissue culture of garlic (allium sativum l.)
url http://hdl.handle.net/20.500.12110/paper_10545476_v38_n6_p608_Parisi
work_keys_str_mv AT parisim characterizationofanovelcysteinepeptidasefromtissuecultureofgarlicalliumsativuml
AT morenos characterizationofanovelcysteinepeptidasefromtissuecultureofgarlicalliumsativuml
AT fernandezc characterizationofanovelcysteinepeptidasefromtissuecultureofgarlicalliumsativuml
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