Spectrophotometric assay using o-phthaldialdehyde for determination of reactive lysine in dairy products

The applicability of a simple and rapid spectrophotometric assay using o-phthaldialdehyde (OPA) to determine reactive lysine in dairy products was evaluated. For this purpose the values of reactive lysine of model systems and dairy products obtained by this procedure were compared with those obtaine...

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Autores principales:	Vigo, M.S., Malec, L.S., Gomez, R.G., Llosa, R.A.
Formato:	JOUR
Materias:	1 fluoro 2,4 dinitrobenzene lysine phthalaldehyde article dairy product intermethod comparison spectrophotometry
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03088146_v44_n5_p363_Vigo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_03088146_v44_n5_p363_Vigo2023-10-03T15:22:57Z Spectrophotometric assay using o-phthaldialdehyde for determination of reactive lysine in dairy products Vigo, M.S. Malec, L.S. Gomez, R.G. Llosa, R.A. 1 fluoro 2,4 dinitrobenzene lysine phthalaldehyde article dairy product intermethod comparison spectrophotometry The applicability of a simple and rapid spectrophotometric assay using o-phthaldialdehyde (OPA) to determine reactive lysine in dairy products was evaluated. For this purpose the values of reactive lysine of model systems and dairy products obtained by this procedure were compared with those obtained by the 1-fluoro-2,4-dinitrobenzene (FDNB) method in dialyzed and non-dialyzed samples. As a reference the latter method was applied to dialyzed samples to avoid interference of sugars. The results obtained showed that the OPA procedure is reliable for use even in the presence of high levels of sugars and can also be used to evaluate lysine damage after Maillard-type reactions. © 1992. Fil:Vigo, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Malec, L.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gomez, R.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Llosa, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03088146_v44_n5_p363_Vigo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	1 fluoro 2,4 dinitrobenzene lysine phthalaldehyde article dairy product intermethod comparison spectrophotometry
spellingShingle	1 fluoro 2,4 dinitrobenzene lysine phthalaldehyde article dairy product intermethod comparison spectrophotometry Vigo, M.S. Malec, L.S. Gomez, R.G. Llosa, R.A. Spectrophotometric assay using o-phthaldialdehyde for determination of reactive lysine in dairy products
topic_facet	1 fluoro 2,4 dinitrobenzene lysine phthalaldehyde article dairy product intermethod comparison spectrophotometry
description	The applicability of a simple and rapid spectrophotometric assay using o-phthaldialdehyde (OPA) to determine reactive lysine in dairy products was evaluated. For this purpose the values of reactive lysine of model systems and dairy products obtained by this procedure were compared with those obtained by the 1-fluoro-2,4-dinitrobenzene (FDNB) method in dialyzed and non-dialyzed samples. As a reference the latter method was applied to dialyzed samples to avoid interference of sugars. The results obtained showed that the OPA procedure is reliable for use even in the presence of high levels of sugars and can also be used to evaluate lysine damage after Maillard-type reactions. © 1992.
format	JOUR
author	Vigo, M.S. Malec, L.S. Gomez, R.G. Llosa, R.A.
author_facet	Vigo, M.S. Malec, L.S. Gomez, R.G. Llosa, R.A.
author_sort	Vigo, M.S.
title	Spectrophotometric assay using o-phthaldialdehyde for determination of reactive lysine in dairy products
title_short	Spectrophotometric assay using o-phthaldialdehyde for determination of reactive lysine in dairy products
title_full	Spectrophotometric assay using o-phthaldialdehyde for determination of reactive lysine in dairy products
title_fullStr	Spectrophotometric assay using o-phthaldialdehyde for determination of reactive lysine in dairy products
title_full_unstemmed	Spectrophotometric assay using o-phthaldialdehyde for determination of reactive lysine in dairy products
title_sort	spectrophotometric assay using o-phthaldialdehyde for determination of reactive lysine in dairy products
url	http://hdl.handle.net/20.500.12110/paper_03088146_v44_n5_p363_Vigo
work_keys_str_mv	AT vigoms spectrophotometricassayusingophthaldialdehydefordeterminationofreactivelysineindairyproducts AT malecls spectrophotometricassayusingophthaldialdehydefordeterminationofreactivelysineindairyproducts AT gomezrg spectrophotometricassayusingophthaldialdehydefordeterminationofreactivelysineindairyproducts AT llosara spectrophotometricassayusingophthaldialdehydefordeterminationofreactivelysineindairyproducts
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Spectrophotometric assay using o-phthaldialdehyde for determination of reactive lysine in dairy products

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