Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems

Whey protein concentrate (WPC) was fractionated by using hydroxypropylmethylcellulose (HPMC) at pH 6.5. Incompatible mixtures with different proportions of HPMC and WPC were prepared. After phase separation, the protein concentration in both phases was determined by the Kjeldahl method and the propo...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Jara, F., Pilosof, A.M.R.
Formato: JOUR
Materias:
Hydroxypropylmethylcellulose
Phase separation
Protein partitioning
Thermodynamic incompatibility
Whey proteins
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0268005X_v25_n3_p374_Jara
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_0268005X_v25_n3_p374_Jara
record_format dspace
spelling todo:paper_0268005X_v25_n3_p374_Jara2023-10-03T15:13:39Z Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems Jara, F. Pilosof, A.M.R. Hydroxypropylmethylcellulose Phase separation Protein partitioning Thermodynamic incompatibility Whey proteins Whey protein concentrate (WPC) was fractionated by using hydroxypropylmethylcellulose (HPMC) at pH 6.5. Incompatible mixtures with different proportions of HPMC and WPC were prepared. After phase separation, the protein concentration in both phases was determined by the Kjeldahl method and the proportion of each protein by SDS-PAGE combined with image analysis. The results show that the low molecular weight proteins α-lactalbumin (α-lac) and β-lactoglobulin (β-lg) were retained in high proportion in the upper phase (about 90% compared to 64% of WPC). The most efficient condition to fractionate β-lg and α-lac was the phase separation of an incompatible mixed system with a high initial concentration of WPC and a low initial concentration of HPMC i.e., WPC 20%, wt/wt/HPMC 0.5%, w/w. It can be concluded that the thermodynamic incompatibility which arises from mixing WPC with HPMC could be used as a method for fractionation of whey proteins. © 2010 Elsevier Ltd. Fil:Jara, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0268005X_v25_n3_p374_Jara
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Hydroxypropylmethylcellulose
Phase separation
Protein partitioning
Thermodynamic incompatibility
Whey proteins
spellingShingle Hydroxypropylmethylcellulose
Phase separation
Protein partitioning
Thermodynamic incompatibility
Whey proteins
Jara, F.
Pilosof, A.M.R.
Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems
topic_facet Hydroxypropylmethylcellulose
Phase separation
Protein partitioning
Thermodynamic incompatibility
Whey proteins
description Whey protein concentrate (WPC) was fractionated by using hydroxypropylmethylcellulose (HPMC) at pH 6.5. Incompatible mixtures with different proportions of HPMC and WPC were prepared. After phase separation, the protein concentration in both phases was determined by the Kjeldahl method and the proportion of each protein by SDS-PAGE combined with image analysis. The results show that the low molecular weight proteins α-lactalbumin (α-lac) and β-lactoglobulin (β-lg) were retained in high proportion in the upper phase (about 90% compared to 64% of WPC). The most efficient condition to fractionate β-lg and α-lac was the phase separation of an incompatible mixed system with a high initial concentration of WPC and a low initial concentration of HPMC i.e., WPC 20%, wt/wt/HPMC 0.5%, w/w. It can be concluded that the thermodynamic incompatibility which arises from mixing WPC with HPMC could be used as a method for fractionation of whey proteins. © 2010 Elsevier Ltd.
format JOUR
author Jara, F.
Pilosof, A.M.R.
author_facet Jara, F.
Pilosof, A.M.R.
author_sort Jara, F.
title Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems
title_short Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems
title_full Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems
title_fullStr Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems
title_full_unstemmed Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems
title_sort partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems
url http://hdl.handle.net/20.500.12110/paper_0268005X_v25_n3_p374_Jara
work_keys_str_mv AT jaraf partitioningofalactalbuminandblactoglobulininwheyproteinconcentratehydroxypropylmethylcelluloseaqueoustwophasesystems
AT pilosofamr partitioningofalactalbuminandblactoglobulininwheyproteinconcentratehydroxypropylmethylcelluloseaqueoustwophasesystems
_version_ 1782029016931237888

Ejemplares similares