Interaction of Trypanosoma cruzi adenylate cyclase with liver regulatory factors
Trypanosoma cruzi adenylate cyclase subunits may interact with regulatory factors from rat liver membranes, reconstituting heterologous systems which are catalytically active in assay mixtures containing MgATP. The systems show stimulatory responses to glucagon and guanosine 5'-[βγ-imido]tripho...
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| Autores principales: | , , , |
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_02646021_v236_n1_p185_Eisenschlos |
| Aporte de: |
| Sumario: | Trypanosoma cruzi adenylate cyclase subunits may interact with regulatory factors from rat liver membranes, reconstituting heterologous systems which are catalytically active in assay mixtures containing MgATP. The systems show stimulatory responses to glucagon and guanosine 5'-[βγ-imido]triphosphate (p[NH]ppG) or fluoride. Reconstitution was obtained by three different methods: (1) fusion of rat liver membranes (pretreated with N-ethylmaleimide) to T. cruzi membranes; (2) interaction of detergent extracts of rat liver membranes with T. cruzi membranes; or (3) interaction of purified preparations of T. cruzi adenylate cyclase and of liver membrane factors in phospholipid vesicles. The liver factors responsible for the guanine nucleotide effect were characterized as the N8 protein. Data also indicate that reconstitution requires the presence of a membrane substrate. |
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