Interaction of Trypanosoma cruzi adenylate cyclase with liver regulatory factors

Trypanosoma cruzi adenylate cyclase subunits may interact with regulatory factors from rat liver membranes, reconstituting heterologous systems which are catalytically active in assay mixtures containing MgATP. The systems show stimulatory responses to glucagon and guanosine 5'-[βγ-imido]tripho...

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Autores principales: Eisenschlos, C., Flawia, M.M., Torruella, M., Torres, H.N.
Formato: JOUR
Materias:
adenylate cyclase
glucagon
guanylylimidodiphosphate
animal cell
liver
nonhuman
priority journal
protozoon
rat
Trypanosoma cruzi
Animalia
Protozoa
Trypanosoma
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02646021_v236_n1_p185_Eisenschlos
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_02646021_v236_n1_p185_Eisenschlos
record_format dspace
spelling todo:paper_02646021_v236_n1_p185_Eisenschlos2023-10-03T15:12:54Z Interaction of Trypanosoma cruzi adenylate cyclase with liver regulatory factors Eisenschlos, C. Flawia, M.M. Torruella, M. Torres, H.N. adenylate cyclase glucagon guanylylimidodiphosphate animal cell liver nonhuman priority journal protozoon rat Trypanosoma cruzi Animalia Protozoa Trypanosoma Trypanosoma cruzi Trypanosoma cruzi adenylate cyclase subunits may interact with regulatory factors from rat liver membranes, reconstituting heterologous systems which are catalytically active in assay mixtures containing MgATP. The systems show stimulatory responses to glucagon and guanosine 5'-[βγ-imido]triphosphate (p[NH]ppG) or fluoride. Reconstitution was obtained by three different methods: (1) fusion of rat liver membranes (pretreated with N-ethylmaleimide) to T. cruzi membranes; (2) interaction of detergent extracts of rat liver membranes with T. cruzi membranes; or (3) interaction of purified preparations of T. cruzi adenylate cyclase and of liver membrane factors in phospholipid vesicles. The liver factors responsible for the guanine nucleotide effect were characterized as the N8 protein. Data also indicate that reconstitution requires the presence of a membrane substrate. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02646021_v236_n1_p185_Eisenschlos
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic adenylate cyclase
glucagon
guanylylimidodiphosphate
animal cell
liver
nonhuman
priority journal
protozoon
rat
Trypanosoma cruzi
Animalia
Protozoa
Trypanosoma
Trypanosoma cruzi
spellingShingle adenylate cyclase
glucagon
guanylylimidodiphosphate
animal cell
liver
nonhuman
priority journal
protozoon
rat
Trypanosoma cruzi
Animalia
Protozoa
Trypanosoma
Trypanosoma cruzi
Eisenschlos, C.
Flawia, M.M.
Torruella, M.
Torres, H.N.
Interaction of Trypanosoma cruzi adenylate cyclase with liver regulatory factors
topic_facet adenylate cyclase
glucagon
guanylylimidodiphosphate
animal cell
liver
nonhuman
priority journal
protozoon
rat
Trypanosoma cruzi
Animalia
Protozoa
Trypanosoma
Trypanosoma cruzi
description Trypanosoma cruzi adenylate cyclase subunits may interact with regulatory factors from rat liver membranes, reconstituting heterologous systems which are catalytically active in assay mixtures containing MgATP. The systems show stimulatory responses to glucagon and guanosine 5'-[βγ-imido]triphosphate (p[NH]ppG) or fluoride. Reconstitution was obtained by three different methods: (1) fusion of rat liver membranes (pretreated with N-ethylmaleimide) to T. cruzi membranes; (2) interaction of detergent extracts of rat liver membranes with T. cruzi membranes; or (3) interaction of purified preparations of T. cruzi adenylate cyclase and of liver membrane factors in phospholipid vesicles. The liver factors responsible for the guanine nucleotide effect were characterized as the N8 protein. Data also indicate that reconstitution requires the presence of a membrane substrate.
format JOUR
author Eisenschlos, C.
Flawia, M.M.
Torruella, M.
Torres, H.N.
author_facet Eisenschlos, C.
Flawia, M.M.
Torruella, M.
Torres, H.N.
author_sort Eisenschlos, C.
title Interaction of Trypanosoma cruzi adenylate cyclase with liver regulatory factors
title_short Interaction of Trypanosoma cruzi adenylate cyclase with liver regulatory factors
title_full Interaction of Trypanosoma cruzi adenylate cyclase with liver regulatory factors
title_fullStr Interaction of Trypanosoma cruzi adenylate cyclase with liver regulatory factors
title_full_unstemmed Interaction of Trypanosoma cruzi adenylate cyclase with liver regulatory factors
title_sort interaction of trypanosoma cruzi adenylate cyclase with liver regulatory factors
url http://hdl.handle.net/20.500.12110/paper_02646021_v236_n1_p185_Eisenschlos
work_keys_str_mv AT eisenschlosc interactionoftrypanosomacruziadenylatecyclasewithliverregulatoryfactors
AT flawiamm interactionoftrypanosomacruziadenylatecyclasewithliverregulatoryfactors
AT torruellam interactionoftrypanosomacruziadenylatecyclasewithliverregulatoryfactors
AT torreshn interactionoftrypanosomacruziadenylatecyclasewithliverregulatoryfactors
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