Selection of an extracellular esterase-producing microorganism
The conventional esterase plate-screening technique has been modified in order to obtain a better differentiation of high producing strains. A short exposure to Lugol's iodine solution after colony growth enhanced the contrast between the precipitation halo and the background. Batch cultures of...
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todo:paper_01694146_v10_n3-4_p165_Castro2023-10-03T15:07:05Z Selection of an extracellular esterase-producing microorganism Castro, G.R. Stettler, A.O. Ferrero, M.A. Siñeriz, F. B. subtilis esterase Esterase production Esterase screening test pH profile Temperature profile Batch cell culture Biotechnology Enzymes Microbiology Esterase plate screening Esterase producing microorganisms Extracellular esterases Microorganisms esterase article bacillus subtilis microorganism nonhuman ph priority journal screening temperature The conventional esterase plate-screening technique has been modified in order to obtain a better differentiation of high producing strains. A short exposure to Lugol's iodine solution after colony growth enhanced the contrast between the precipitation halo and the background. Batch cultures of a selected strain characterized as belonging to the Bacillus subtilis group showed a high level of extracellular activity at pH 6.6 to 8.0 and 35°C. In crude extracts the optimum enzymatic activity was obtained at 35°C and pH 7.0. © 1992 Society for Industrial Microbiology. Fil:Castro, G.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ferrero, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Siñeriz, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01694146_v10_n3-4_p165_Castro |
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Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
B. subtilis esterase Esterase production Esterase screening test pH profile Temperature profile Batch cell culture Biotechnology Enzymes Microbiology Esterase plate screening Esterase producing microorganisms Extracellular esterases Microorganisms esterase article bacillus subtilis microorganism nonhuman ph priority journal screening temperature |
spellingShingle |
B. subtilis esterase Esterase production Esterase screening test pH profile Temperature profile Batch cell culture Biotechnology Enzymes Microbiology Esterase plate screening Esterase producing microorganisms Extracellular esterases Microorganisms esterase article bacillus subtilis microorganism nonhuman ph priority journal screening temperature Castro, G.R. Stettler, A.O. Ferrero, M.A. Siñeriz, F. Selection of an extracellular esterase-producing microorganism |
topic_facet |
B. subtilis esterase Esterase production Esterase screening test pH profile Temperature profile Batch cell culture Biotechnology Enzymes Microbiology Esterase plate screening Esterase producing microorganisms Extracellular esterases Microorganisms esterase article bacillus subtilis microorganism nonhuman ph priority journal screening temperature |
description |
The conventional esterase plate-screening technique has been modified in order to obtain a better differentiation of high producing strains. A short exposure to Lugol's iodine solution after colony growth enhanced the contrast between the precipitation halo and the background. Batch cultures of a selected strain characterized as belonging to the Bacillus subtilis group showed a high level of extracellular activity at pH 6.6 to 8.0 and 35°C. In crude extracts the optimum enzymatic activity was obtained at 35°C and pH 7.0. © 1992 Society for Industrial Microbiology. |
format |
JOUR |
author |
Castro, G.R. Stettler, A.O. Ferrero, M.A. Siñeriz, F. |
author_facet |
Castro, G.R. Stettler, A.O. Ferrero, M.A. Siñeriz, F. |
author_sort |
Castro, G.R. |
title |
Selection of an extracellular esterase-producing microorganism |
title_short |
Selection of an extracellular esterase-producing microorganism |
title_full |
Selection of an extracellular esterase-producing microorganism |
title_fullStr |
Selection of an extracellular esterase-producing microorganism |
title_full_unstemmed |
Selection of an extracellular esterase-producing microorganism |
title_sort |
selection of an extracellular esterase-producing microorganism |
url |
http://hdl.handle.net/20.500.12110/paper_01694146_v10_n3-4_p165_Castro |
work_keys_str_mv |
AT castrogr selectionofanextracellularesteraseproducingmicroorganism AT stettlerao selectionofanextracellularesteraseproducingmicroorganism AT ferreroma selectionofanextracellularesteraseproducingmicroorganism AT sinerizf selectionofanextracellularesteraseproducingmicroorganism |
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1782025069925498880 |