Selection of an extracellular esterase-producing microorganism

The conventional esterase plate-screening technique has been modified in order to obtain a better differentiation of high producing strains. A short exposure to Lugol's iodine solution after colony growth enhanced the contrast between the precipitation halo and the background. Batch cultures of...

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Autores principales:	Castro, G.R., Stettler, A.O., Ferrero, M.A., Siñeriz, F.
Formato:	JOUR
Materias:	B. subtilis esterase Esterase production Esterase screening test pH profile Temperature profile Batch cell culture Biotechnology Enzymes Microbiology Esterase plate screening Esterase producing microorganisms Extracellular esterases Microorganisms esterase article bacillus subtilis microorganism nonhuman ph priority journal screening temperature
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01694146_v10_n3-4_p165_Castro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01694146_v10_n3-4_p165_Castro
record_format	dspace
spelling	todo:paper_01694146_v10_n3-4_p165_Castro2023-10-03T15:07:05Z Selection of an extracellular esterase-producing microorganism Castro, G.R. Stettler, A.O. Ferrero, M.A. Siñeriz, F. B. subtilis esterase Esterase production Esterase screening test pH profile Temperature profile Batch cell culture Biotechnology Enzymes Microbiology Esterase plate screening Esterase producing microorganisms Extracellular esterases Microorganisms esterase article bacillus subtilis microorganism nonhuman ph priority journal screening temperature The conventional esterase plate-screening technique has been modified in order to obtain a better differentiation of high producing strains. A short exposure to Lugol's iodine solution after colony growth enhanced the contrast between the precipitation halo and the background. Batch cultures of a selected strain characterized as belonging to the Bacillus subtilis group showed a high level of extracellular activity at pH 6.6 to 8.0 and 35°C. In crude extracts the optimum enzymatic activity was obtained at 35°C and pH 7.0. © 1992 Society for Industrial Microbiology. Fil:Castro, G.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ferrero, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Siñeriz, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01694146_v10_n3-4_p165_Castro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	B. subtilis esterase Esterase production Esterase screening test pH profile Temperature profile Batch cell culture Biotechnology Enzymes Microbiology Esterase plate screening Esterase producing microorganisms Extracellular esterases Microorganisms esterase article bacillus subtilis microorganism nonhuman ph priority journal screening temperature
spellingShingle	B. subtilis esterase Esterase production Esterase screening test pH profile Temperature profile Batch cell culture Biotechnology Enzymes Microbiology Esterase plate screening Esterase producing microorganisms Extracellular esterases Microorganisms esterase article bacillus subtilis microorganism nonhuman ph priority journal screening temperature Castro, G.R. Stettler, A.O. Ferrero, M.A. Siñeriz, F. Selection of an extracellular esterase-producing microorganism
topic_facet	B. subtilis esterase Esterase production Esterase screening test pH profile Temperature profile Batch cell culture Biotechnology Enzymes Microbiology Esterase plate screening Esterase producing microorganisms Extracellular esterases Microorganisms esterase article bacillus subtilis microorganism nonhuman ph priority journal screening temperature
description	The conventional esterase plate-screening technique has been modified in order to obtain a better differentiation of high producing strains. A short exposure to Lugol's iodine solution after colony growth enhanced the contrast between the precipitation halo and the background. Batch cultures of a selected strain characterized as belonging to the Bacillus subtilis group showed a high level of extracellular activity at pH 6.6 to 8.0 and 35°C. In crude extracts the optimum enzymatic activity was obtained at 35°C and pH 7.0. © 1992 Society for Industrial Microbiology.
format	JOUR
author	Castro, G.R. Stettler, A.O. Ferrero, M.A. Siñeriz, F.
author_facet	Castro, G.R. Stettler, A.O. Ferrero, M.A. Siñeriz, F.
author_sort	Castro, G.R.
title	Selection of an extracellular esterase-producing microorganism
title_short	Selection of an extracellular esterase-producing microorganism
title_full	Selection of an extracellular esterase-producing microorganism
title_fullStr	Selection of an extracellular esterase-producing microorganism
title_full_unstemmed	Selection of an extracellular esterase-producing microorganism
title_sort	selection of an extracellular esterase-producing microorganism
url	http://hdl.handle.net/20.500.12110/paper_01694146_v10_n3-4_p165_Castro
work_keys_str_mv	AT castrogr selectionofanextracellularesteraseproducingmicroorganism AT stettlerao selectionofanextracellularesteraseproducingmicroorganism AT ferreroma selectionofanextracellularesteraseproducingmicroorganism AT sinerizf selectionofanextracellularesteraseproducingmicroorganism
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Selection of an extracellular esterase-producing microorganism

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