Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure
The correlation between blood lead level (BLL), δ-aminolevulinate dehydratase (ALA-D) activity, and other common biochemical parameters used to assess a plumbism diagnosis have been carefully analyzed, with the aim of correctly interpreting the data handled in the laboratory. No correlation was obse...
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todo:paper_01476513_v32_n3_p267_Polo2023-10-03T15:00:43Z Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure Polo, C.F. Afonso, S.G. Navone, N.M. Rossetti, M.V. Batlle, A.M.D.C. lead porphobilinogen synthase porphyrin zinc article enzyme activity enzyme assay erythrocyte erythropoiesis heme synthesis human human cell lead blood level lead poisoning Adult Alanine Binding Sites Child Child, Preschool Erythrocytes Female Hemoglobins Humans Lead Lead Poisoning Male Porphobilinogen Porphobilinogen Synthase Porphyrins Reference Standards Reproducibility of Results Spectrophotometry, Atomic Zinc The correlation between blood lead level (BLL), δ-aminolevulinate dehydratase (ALA-D) activity, and other common biochemical parameters used to assess a plumbism diagnosis have been carefully analyzed, with the aim of correctly interpreting the data handled in the laboratory. No correlation was observed between BLL and free erythrocyte porphyrins. In the case of ALA-D or Zn-reactivated ALA-D despite the direct correlation with BLL, the curve follows a potential or a logarithmic line, which is not the best to calculate BLL. The so-called Zn-ALA-D-reactivation index (iZn) has been defined as the ratio between the activity of Zn-reactivated ALA-D and the activity of ALA-D. The plot of BLL against the iZn revealed a very good linear relationship which allows an estimate of BLL with reasonable accuracy within a very wide range. © 1995 by Academic Press, Inc. Fil:Afonso, S.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rossetti, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A.M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01476513_v32_n3_p267_Polo |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
lead porphobilinogen synthase porphyrin zinc article enzyme activity enzyme assay erythrocyte erythropoiesis heme synthesis human human cell lead blood level lead poisoning Adult Alanine Binding Sites Child Child, Preschool Erythrocytes Female Hemoglobins Humans Lead Lead Poisoning Male Porphobilinogen Porphobilinogen Synthase Porphyrins Reference Standards Reproducibility of Results Spectrophotometry, Atomic Zinc |
spellingShingle |
lead porphobilinogen synthase porphyrin zinc article enzyme activity enzyme assay erythrocyte erythropoiesis heme synthesis human human cell lead blood level lead poisoning Adult Alanine Binding Sites Child Child, Preschool Erythrocytes Female Hemoglobins Humans Lead Lead Poisoning Male Porphobilinogen Porphobilinogen Synthase Porphyrins Reference Standards Reproducibility of Results Spectrophotometry, Atomic Zinc Polo, C.F. Afonso, S.G. Navone, N.M. Rossetti, M.V. Batlle, A.M.D.C. Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure |
topic_facet |
lead porphobilinogen synthase porphyrin zinc article enzyme activity enzyme assay erythrocyte erythropoiesis heme synthesis human human cell lead blood level lead poisoning Adult Alanine Binding Sites Child Child, Preschool Erythrocytes Female Hemoglobins Humans Lead Lead Poisoning Male Porphobilinogen Porphobilinogen Synthase Porphyrins Reference Standards Reproducibility of Results Spectrophotometry, Atomic Zinc |
description |
The correlation between blood lead level (BLL), δ-aminolevulinate dehydratase (ALA-D) activity, and other common biochemical parameters used to assess a plumbism diagnosis have been carefully analyzed, with the aim of correctly interpreting the data handled in the laboratory. No correlation was observed between BLL and free erythrocyte porphyrins. In the case of ALA-D or Zn-reactivated ALA-D despite the direct correlation with BLL, the curve follows a potential or a logarithmic line, which is not the best to calculate BLL. The so-called Zn-ALA-D-reactivation index (iZn) has been defined as the ratio between the activity of Zn-reactivated ALA-D and the activity of ALA-D. The plot of BLL against the iZn revealed a very good linear relationship which allows an estimate of BLL with reasonable accuracy within a very wide range. © 1995 by Academic Press, Inc. |
format |
JOUR |
author |
Polo, C.F. Afonso, S.G. Navone, N.M. Rossetti, M.V. Batlle, A.M.D.C. |
author_facet |
Polo, C.F. Afonso, S.G. Navone, N.M. Rossetti, M.V. Batlle, A.M.D.C. |
author_sort |
Polo, C.F. |
title |
Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure |
title_short |
Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure |
title_full |
Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure |
title_fullStr |
Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure |
title_full_unstemmed |
Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure |
title_sort |
zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure |
url |
http://hdl.handle.net/20.500.12110/paper_01476513_v32_n3_p267_Polo |
work_keys_str_mv |
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1782024928770392064 |