Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure

The correlation between blood lead level (BLL), δ-aminolevulinate dehydratase (ALA-D) activity, and other common biochemical parameters used to assess a plumbism diagnosis have been carefully analyzed, with the aim of correctly interpreting the data handled in the laboratory. No correlation was obse...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Polo, C.F., Afonso, S.G., Navone, N.M., Rossetti, M.V., Batlle, A.M.D.C.
Formato:	JOUR
Materias:	lead porphobilinogen synthase porphyrin zinc article enzyme activity enzyme assay erythrocyte erythropoiesis heme synthesis human human cell lead blood level lead poisoning Adult Alanine Binding Sites Child Child, Preschool Erythrocytes Female Hemoglobins Humans Lead Lead Poisoning Male Porphobilinogen Porphobilinogen Synthase Porphyrins Reference Standards Reproducibility of Results Spectrophotometry, Atomic Zinc
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01476513_v32_n3_p267_Polo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01476513_v32_n3_p267_Polo
record_format	dspace
spelling	todo:paper_01476513_v32_n3_p267_Polo2023-10-03T15:00:43Z Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure Polo, C.F. Afonso, S.G. Navone, N.M. Rossetti, M.V. Batlle, A.M.D.C. lead porphobilinogen synthase porphyrin zinc article enzyme activity enzyme assay erythrocyte erythropoiesis heme synthesis human human cell lead blood level lead poisoning Adult Alanine Binding Sites Child Child, Preschool Erythrocytes Female Hemoglobins Humans Lead Lead Poisoning Male Porphobilinogen Porphobilinogen Synthase Porphyrins Reference Standards Reproducibility of Results Spectrophotometry, Atomic Zinc The correlation between blood lead level (BLL), δ-aminolevulinate dehydratase (ALA-D) activity, and other common biochemical parameters used to assess a plumbism diagnosis have been carefully analyzed, with the aim of correctly interpreting the data handled in the laboratory. No correlation was observed between BLL and free erythrocyte porphyrins. In the case of ALA-D or Zn-reactivated ALA-D despite the direct correlation with BLL, the curve follows a potential or a logarithmic line, which is not the best to calculate BLL. The so-called Zn-ALA-D-reactivation index (iZn) has been defined as the ratio between the activity of Zn-reactivated ALA-D and the activity of ALA-D. The plot of BLL against the iZn revealed a very good linear relationship which allows an estimate of BLL with reasonable accuracy within a very wide range. © 1995 by Academic Press, Inc. Fil:Afonso, S.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rossetti, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A.M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01476513_v32_n3_p267_Polo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	lead porphobilinogen synthase porphyrin zinc article enzyme activity enzyme assay erythrocyte erythropoiesis heme synthesis human human cell lead blood level lead poisoning Adult Alanine Binding Sites Child Child, Preschool Erythrocytes Female Hemoglobins Humans Lead Lead Poisoning Male Porphobilinogen Porphobilinogen Synthase Porphyrins Reference Standards Reproducibility of Results Spectrophotometry, Atomic Zinc
spellingShingle	lead porphobilinogen synthase porphyrin zinc article enzyme activity enzyme assay erythrocyte erythropoiesis heme synthesis human human cell lead blood level lead poisoning Adult Alanine Binding Sites Child Child, Preschool Erythrocytes Female Hemoglobins Humans Lead Lead Poisoning Male Porphobilinogen Porphobilinogen Synthase Porphyrins Reference Standards Reproducibility of Results Spectrophotometry, Atomic Zinc Polo, C.F. Afonso, S.G. Navone, N.M. Rossetti, M.V. Batlle, A.M.D.C. Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure
topic_facet	lead porphobilinogen synthase porphyrin zinc article enzyme activity enzyme assay erythrocyte erythropoiesis heme synthesis human human cell lead blood level lead poisoning Adult Alanine Binding Sites Child Child, Preschool Erythrocytes Female Hemoglobins Humans Lead Lead Poisoning Male Porphobilinogen Porphobilinogen Synthase Porphyrins Reference Standards Reproducibility of Results Spectrophotometry, Atomic Zinc
description	The correlation between blood lead level (BLL), δ-aminolevulinate dehydratase (ALA-D) activity, and other common biochemical parameters used to assess a plumbism diagnosis have been carefully analyzed, with the aim of correctly interpreting the data handled in the laboratory. No correlation was observed between BLL and free erythrocyte porphyrins. In the case of ALA-D or Zn-reactivated ALA-D despite the direct correlation with BLL, the curve follows a potential or a logarithmic line, which is not the best to calculate BLL. The so-called Zn-ALA-D-reactivation index (iZn) has been defined as the ratio between the activity of Zn-reactivated ALA-D and the activity of ALA-D. The plot of BLL against the iZn revealed a very good linear relationship which allows an estimate of BLL with reasonable accuracy within a very wide range. © 1995 by Academic Press, Inc.
format	JOUR
author	Polo, C.F. Afonso, S.G. Navone, N.M. Rossetti, M.V. Batlle, A.M.D.C.
author_facet	Polo, C.F. Afonso, S.G. Navone, N.M. Rossetti, M.V. Batlle, A.M.D.C.
author_sort	Polo, C.F.
title	Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure
title_short	Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure
title_full	Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure
title_fullStr	Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure
title_full_unstemmed	Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure
title_sort	zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure
url	http://hdl.handle.net/20.500.12110/paper_01476513_v32_n3_p267_Polo
work_keys_str_mv	AT polocf zincaminolevulinicaciddehydratasereactivationindexasatoolfordiagnosisofleadexposure AT afonsosg zincaminolevulinicaciddehydratasereactivationindexasatoolfordiagnosisofleadexposure AT navonenm zincaminolevulinicaciddehydratasereactivationindexasatoolfordiagnosisofleadexposure AT rossettimv zincaminolevulinicaciddehydratasereactivationindexasatoolfordiagnosisofleadexposure AT batlleamdc zincaminolevulinicaciddehydratasereactivationindexasatoolfordiagnosisofleadexposure
_version_	1782024928770392064

Zinc aminolevulinic acid dehydratase reactivation index as a tool for diagnosis of lead exposure

Ejemplares similares