Inhibitory effect and cell damage on bacterial flora of fish caused by chitosan, nisin and sodium lactate
The effect of the combined use of chitosan, nisin and sodium lactate on the growth of Listeria innocua, Shewanella putrefaciens and psychrophilic bacteria isolated from fish was investigated in broth by means of minimum inhibitory concentrations (MIC). Furthermore, the sites of cell-injury caused by...
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todo:paper_01418130_v83_n_p396_Schelegueda2023-10-03T14:58:48Z Inhibitory effect and cell damage on bacterial flora of fish caused by chitosan, nisin and sodium lactate Schelegueda, L.I. Zalazar, A.L. Gliemmo, M.F. Campos, C.A. Chitosan Nisin Sodium lactate bacterial DNA chitosan lactate sodium nisin antiinfective agent chitosan lactate sodium nisin animal cell Article bacterial cell bacterial flora bacterial growth bacterial membrane bacterial structures cell damage cell surface chemical interaction controlled study differential scanning calorimetry fish food preservation food spoilage growth inhibition hydrophobicity Listeria innocua minimum inhibitory concentration nonhuman psychrophilic bacterium ribosome Shewanella putrefaciens animal bacterium chemical phenomena cytology drug effects drug interaction growth, development and aging metabolism microbial sensitivity test microbiology Animals Anti-Bacterial Agents Bacteria Chitosan Drug Interactions Fishes Hydrophobic and Hydrophilic Interactions Microbial Sensitivity Tests Nisin Sodium Lactate The effect of the combined use of chitosan, nisin and sodium lactate on the growth of Listeria innocua, Shewanella putrefaciens and psychrophilic bacteria isolated from fish was investigated in broth by means of minimum inhibitory concentrations (MIC). Furthermore, the sites of cell-injury caused by mentioned antimicrobials and their combinations on L. innocua and S. putrefaciens were studied. MIC of antimicrobial mixtures were evaluated by Berembaum design and check board method. Antimicrobials' sites of injury were investigated by the evaluation of cell constituents' release, cell surface hydrophobicity and differential scanning calorimetry. Results depended on antimicrobial used; several combinations inhibited the growth of L. innocua and S. putrefaciens and all combinations inhibited psychrophilic bacteria. Besides, some mixtures showed synergistic effects. All the mixtures affected ribosomes and DNA of the studied bacteria. Regarding cellular envelope, antimicrobials acted according to the structural characteristics of target microorganisms. Cell damage was higher when antimicrobials were combined, which could explain the observed synergistic effects. This study demonstrates and justifies the synergistic action of chitosan, nisin and sodium lactate on the inhibition of microorganisms related to fish spoilage and remarks the promissory use of the synergic combination of antimicrobials for fish preservation. © 2015 Elsevier B.V. Fil:Gliemmo, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Campos, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01418130_v83_n_p396_Schelegueda |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chitosan Nisin Sodium lactate bacterial DNA chitosan lactate sodium nisin antiinfective agent chitosan lactate sodium nisin animal cell Article bacterial cell bacterial flora bacterial growth bacterial membrane bacterial structures cell damage cell surface chemical interaction controlled study differential scanning calorimetry fish food preservation food spoilage growth inhibition hydrophobicity Listeria innocua minimum inhibitory concentration nonhuman psychrophilic bacterium ribosome Shewanella putrefaciens animal bacterium chemical phenomena cytology drug effects drug interaction growth, development and aging metabolism microbial sensitivity test microbiology Animals Anti-Bacterial Agents Bacteria Chitosan Drug Interactions Fishes Hydrophobic and Hydrophilic Interactions Microbial Sensitivity Tests Nisin Sodium Lactate |
| spellingShingle |
Chitosan Nisin Sodium lactate bacterial DNA chitosan lactate sodium nisin antiinfective agent chitosan lactate sodium nisin animal cell Article bacterial cell bacterial flora bacterial growth bacterial membrane bacterial structures cell damage cell surface chemical interaction controlled study differential scanning calorimetry fish food preservation food spoilage growth inhibition hydrophobicity Listeria innocua minimum inhibitory concentration nonhuman psychrophilic bacterium ribosome Shewanella putrefaciens animal bacterium chemical phenomena cytology drug effects drug interaction growth, development and aging metabolism microbial sensitivity test microbiology Animals Anti-Bacterial Agents Bacteria Chitosan Drug Interactions Fishes Hydrophobic and Hydrophilic Interactions Microbial Sensitivity Tests Nisin Sodium Lactate Schelegueda, L.I. Zalazar, A.L. Gliemmo, M.F. Campos, C.A. Inhibitory effect and cell damage on bacterial flora of fish caused by chitosan, nisin and sodium lactate |
| topic_facet |
Chitosan Nisin Sodium lactate bacterial DNA chitosan lactate sodium nisin antiinfective agent chitosan lactate sodium nisin animal cell Article bacterial cell bacterial flora bacterial growth bacterial membrane bacterial structures cell damage cell surface chemical interaction controlled study differential scanning calorimetry fish food preservation food spoilage growth inhibition hydrophobicity Listeria innocua minimum inhibitory concentration nonhuman psychrophilic bacterium ribosome Shewanella putrefaciens animal bacterium chemical phenomena cytology drug effects drug interaction growth, development and aging metabolism microbial sensitivity test microbiology Animals Anti-Bacterial Agents Bacteria Chitosan Drug Interactions Fishes Hydrophobic and Hydrophilic Interactions Microbial Sensitivity Tests Nisin Sodium Lactate |
| description |
The effect of the combined use of chitosan, nisin and sodium lactate on the growth of Listeria innocua, Shewanella putrefaciens and psychrophilic bacteria isolated from fish was investigated in broth by means of minimum inhibitory concentrations (MIC). Furthermore, the sites of cell-injury caused by mentioned antimicrobials and their combinations on L. innocua and S. putrefaciens were studied. MIC of antimicrobial mixtures were evaluated by Berembaum design and check board method. Antimicrobials' sites of injury were investigated by the evaluation of cell constituents' release, cell surface hydrophobicity and differential scanning calorimetry. Results depended on antimicrobial used; several combinations inhibited the growth of L. innocua and S. putrefaciens and all combinations inhibited psychrophilic bacteria. Besides, some mixtures showed synergistic effects. All the mixtures affected ribosomes and DNA of the studied bacteria. Regarding cellular envelope, antimicrobials acted according to the structural characteristics of target microorganisms. Cell damage was higher when antimicrobials were combined, which could explain the observed synergistic effects. This study demonstrates and justifies the synergistic action of chitosan, nisin and sodium lactate on the inhibition of microorganisms related to fish spoilage and remarks the promissory use of the synergic combination of antimicrobials for fish preservation. © 2015 Elsevier B.V. |
| format |
JOUR |
| author |
Schelegueda, L.I. Zalazar, A.L. Gliemmo, M.F. Campos, C.A. |
| author_facet |
Schelegueda, L.I. Zalazar, A.L. Gliemmo, M.F. Campos, C.A. |
| author_sort |
Schelegueda, L.I. |
| title |
Inhibitory effect and cell damage on bacterial flora of fish caused by chitosan, nisin and sodium lactate |
| title_short |
Inhibitory effect and cell damage on bacterial flora of fish caused by chitosan, nisin and sodium lactate |
| title_full |
Inhibitory effect and cell damage on bacterial flora of fish caused by chitosan, nisin and sodium lactate |
| title_fullStr |
Inhibitory effect and cell damage on bacterial flora of fish caused by chitosan, nisin and sodium lactate |
| title_full_unstemmed |
Inhibitory effect and cell damage on bacterial flora of fish caused by chitosan, nisin and sodium lactate |
| title_sort |
inhibitory effect and cell damage on bacterial flora of fish caused by chitosan, nisin and sodium lactate |
| url |
http://hdl.handle.net/20.500.12110/paper_01418130_v83_n_p396_Schelegueda |
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