Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis

The aims of this study were to characterize an enterococci bacteriocinogenic strain and to evaluate its antimicrobial activity regarding its potential use for fish preservation. The bacterium, isolated from Odontesthes platensis in the Patagonian region of Argentine was identified as Enterococcus mu...

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Autores principales: Schelegueda, L.I., Vallejo, M., Gliemmo, M.F., Marguet, E.R., Campos, C.A.
Formato: JOUR
Materias:
Bacteriocin
Enterococcus mundtii
Fish preservation
Bacteria
Chitin
Chitosan
Differential scanning calorimetry
Diseases
Fish
Genes
Listeria
Microorganisms
pH effects
Anti-microbial activity
Antimicrobial action
Bacteriocins
Inhibitory concentration
Listeria monocytogenes
Pathogenic microorganisms
Phylogenetic analysis
Spoilage
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00236438_v64_n2_p794_Schelegueda
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00236438_v64_n2_p794_Schelegueda
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spelling todo:paper_00236438_v64_n2_p794_Schelegueda2023-10-03T14:34:18Z Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis Schelegueda, L.I. Vallejo, M. Gliemmo, M.F. Marguet, E.R. Campos, C.A. Bacteriocin Enterococcus mundtii Fish preservation Bacteria Chitin Chitosan Differential scanning calorimetry Diseases Fish Genes Listeria Microorganisms pH effects Anti-microbial activity Antimicrobial action Bacteriocins Enterococcus mundtii Inhibitory concentration Listeria monocytogenes Pathogenic microorganisms Phylogenetic analysis Spoilage The aims of this study were to characterize an enterococci bacteriocinogenic strain and to evaluate its antimicrobial activity regarding its potential use for fish preservation. The bacterium, isolated from Odontesthes platensis in the Patagonian region of Argentine was identified as Enterococcus mundtii by means of biochemical test and 16S rDNA gene phylogenetic analyses. Furthermore, the gene that codifies mundticin KS was detected by PCR techniques. The bacteriocin was able to inhibit Gram positive and negative bacteria. In particular at pH 5.50, it was able to control the growth of fish bacterial flora. Construction of isobolograms and value of fractional inhibitory concentrations index showed that its use together with chitosan and sodium lactate (SL) exerted a synergic action on the inhibition of Listeria innocua, Shewanella putrefaciens and the psychrophilic flora isolated from fish. The use of the mentioned mixture of antimicrobials promoted strong changes on L. innocua and S. putrefaciens cellular components as shown by differential scanning calorimetry. Results suggest that the combination of the bacteriocin with chitosan and SL would be useful to reduce the amount of antimicrobials added to foods and to prevent the growth of spoilage bacteria and pathogenic microorganisms, such as Listeria monocytogenes. © 2015 Elsevier Ltd. Fil:Gliemmo, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Campos, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00236438_v64_n2_p794_Schelegueda
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Bacteriocin
Enterococcus mundtii
Fish preservation
Bacteria
Chitin
Chitosan
Differential scanning calorimetry
Diseases
Fish
Genes
Listeria
Microorganisms
pH effects
Anti-microbial activity
Antimicrobial action
Bacteriocins
Enterococcus mundtii
Inhibitory concentration
Listeria monocytogenes
Pathogenic microorganisms
Phylogenetic analysis
Spoilage
spellingShingle Bacteriocin
Enterococcus mundtii
Fish preservation
Bacteria
Chitin
Chitosan
Differential scanning calorimetry
Diseases
Fish
Genes
Listeria
Microorganisms
pH effects
Anti-microbial activity
Antimicrobial action
Bacteriocins
Enterococcus mundtii
Inhibitory concentration
Listeria monocytogenes
Pathogenic microorganisms
Phylogenetic analysis
Spoilage
Schelegueda, L.I.
Vallejo, M.
Gliemmo, M.F.
Marguet, E.R.
Campos, C.A.
Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
topic_facet Bacteriocin
Enterococcus mundtii
Fish preservation
Bacteria
Chitin
Chitosan
Differential scanning calorimetry
Diseases
Fish
Genes
Listeria
Microorganisms
pH effects
Anti-microbial activity
Antimicrobial action
Bacteriocins
Enterococcus mundtii
Inhibitory concentration
Listeria monocytogenes
Pathogenic microorganisms
Phylogenetic analysis
Spoilage
description The aims of this study were to characterize an enterococci bacteriocinogenic strain and to evaluate its antimicrobial activity regarding its potential use for fish preservation. The bacterium, isolated from Odontesthes platensis in the Patagonian region of Argentine was identified as Enterococcus mundtii by means of biochemical test and 16S rDNA gene phylogenetic analyses. Furthermore, the gene that codifies mundticin KS was detected by PCR techniques. The bacteriocin was able to inhibit Gram positive and negative bacteria. In particular at pH 5.50, it was able to control the growth of fish bacterial flora. Construction of isobolograms and value of fractional inhibitory concentrations index showed that its use together with chitosan and sodium lactate (SL) exerted a synergic action on the inhibition of Listeria innocua, Shewanella putrefaciens and the psychrophilic flora isolated from fish. The use of the mentioned mixture of antimicrobials promoted strong changes on L. innocua and S. putrefaciens cellular components as shown by differential scanning calorimetry. Results suggest that the combination of the bacteriocin with chitosan and SL would be useful to reduce the amount of antimicrobials added to foods and to prevent the growth of spoilage bacteria and pathogenic microorganisms, such as Listeria monocytogenes. © 2015 Elsevier Ltd.
format JOUR
author Schelegueda, L.I.
Vallejo, M.
Gliemmo, M.F.
Marguet, E.R.
Campos, C.A.
author_facet Schelegueda, L.I.
Vallejo, M.
Gliemmo, M.F.
Marguet, E.R.
Campos, C.A.
author_sort Schelegueda, L.I.
title Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
title_short Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
title_full Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
title_fullStr Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
title_full_unstemmed Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis
title_sort synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by enterococcus mundtii isolated from odontesthes platensis
url http://hdl.handle.net/20.500.12110/paper_00236438_v64_n2_p794_Schelegueda
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