A Dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions
In this work, the complex assembly of one of the major storage proteins in soybean, glycinin, was analyzed using dynamic light scattering, from the hydrodynamic diameter of assembled forms in solution. The protein concentration and temperature were maintained constant at 10-1% w/w and 20 °C, respect...
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todo:paper_0003021X_v89_n7_p1183_Victor2023-10-03T13:55:36Z A Dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions Víctor, M. Ruiz-Henestrosa, P. Martinez, M.J. Patino, J.M.R. Pilosof, A.M.R. Dynamic light scattering Globulin Soy Structure Complex assembly Complex equilibria Globulin Glycinin Hydrodynamic diameter Onset temperature Protein concentrations Self-assembled Soy Soy globulin Storage proteins Volume size distribution Hydrodynamics Ionic strength Structure (composition) Dynamic light scattering Glycine max In this work, the complex assembly of one of the major storage proteins in soybean, glycinin, was analyzed using dynamic light scattering, from the hydrodynamic diameter of assembled forms in solution. The protein concentration and temperature were maintained constant at 10-1% w/w and 20 °C, respectively, and the pH was 7.6, 7.0 and 3.0. By analyzing the intensity and volume size distributions, a complex equilibrium between self-assembled forms could be determined. At pH 7.6 and an ionic strength of 0.5 M, where the self-assembly of glycinin has been widely reported in the literature, the DLS technique revealed an equilibrium between different assembled forms, that shifted towards the 11S form. At a lower ionic strength for pH 3.0 or 7.0, the 7S form predominated. The hydrodynamic diameter evolved differently upon heating, depending on pH and ionic strength. For pH 7 (I = 0.05) and 7.6 (I = 0.5) a significant increase in d H was observed at a temperatures of 55 and 70 °C, respectively, which were significantly lower than the denaturation onset temperatures as determined by DSC. No changes in dH nor a transition endotherm were observed at pH 3. © 2012 AOCS. Fil:Martinez, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0003021X_v89_n7_p1183_Victor |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Dynamic light scattering Globulin Soy Structure Complex assembly Complex equilibria Globulin Glycinin Hydrodynamic diameter Onset temperature Protein concentrations Self-assembled Soy Soy globulin Storage proteins Volume size distribution Hydrodynamics Ionic strength Structure (composition) Dynamic light scattering Glycine max |
| spellingShingle |
Dynamic light scattering Globulin Soy Structure Complex assembly Complex equilibria Globulin Glycinin Hydrodynamic diameter Onset temperature Protein concentrations Self-assembled Soy Soy globulin Storage proteins Volume size distribution Hydrodynamics Ionic strength Structure (composition) Dynamic light scattering Glycine max Víctor, M. Ruiz-Henestrosa, P. Martinez, M.J. Patino, J.M.R. Pilosof, A.M.R. A Dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions |
| topic_facet |
Dynamic light scattering Globulin Soy Structure Complex assembly Complex equilibria Globulin Glycinin Hydrodynamic diameter Onset temperature Protein concentrations Self-assembled Soy Soy globulin Storage proteins Volume size distribution Hydrodynamics Ionic strength Structure (composition) Dynamic light scattering Glycine max |
| description |
In this work, the complex assembly of one of the major storage proteins in soybean, glycinin, was analyzed using dynamic light scattering, from the hydrodynamic diameter of assembled forms in solution. The protein concentration and temperature were maintained constant at 10-1% w/w and 20 °C, respectively, and the pH was 7.6, 7.0 and 3.0. By analyzing the intensity and volume size distributions, a complex equilibrium between self-assembled forms could be determined. At pH 7.6 and an ionic strength of 0.5 M, where the self-assembly of glycinin has been widely reported in the literature, the DLS technique revealed an equilibrium between different assembled forms, that shifted towards the 11S form. At a lower ionic strength for pH 3.0 or 7.0, the 7S form predominated. The hydrodynamic diameter evolved differently upon heating, depending on pH and ionic strength. For pH 7 (I = 0.05) and 7.6 (I = 0.5) a significant increase in d H was observed at a temperatures of 55 and 70 °C, respectively, which were significantly lower than the denaturation onset temperatures as determined by DSC. No changes in dH nor a transition endotherm were observed at pH 3. © 2012 AOCS. |
| format |
JOUR |
| author |
Víctor, M. Ruiz-Henestrosa, P. Martinez, M.J. Patino, J.M.R. Pilosof, A.M.R. |
| author_facet |
Víctor, M. Ruiz-Henestrosa, P. Martinez, M.J. Patino, J.M.R. Pilosof, A.M.R. |
| author_sort |
Víctor, M. |
| title |
A Dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions |
| title_short |
A Dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions |
| title_full |
A Dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions |
| title_fullStr |
A Dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions |
| title_full_unstemmed |
A Dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions |
| title_sort |
dynamic light scattering study on the complex assembly of glycinin soy globulin in aqueous solutions |
| url |
http://hdl.handle.net/20.500.12110/paper_0003021X_v89_n7_p1183_Victor |
| work_keys_str_mv |
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