A synthetic heparan sulfate pentasaccharide, exclusively containing L-iduronic acid, displays higher affinity for FGF-2 than its D-glucuronic acid-containing isomers

It has been suggested that the FGF-2 binding site on heparan sulfate chains is a trisulfated pentasaccharide containing three hexuronic acid units. The configuration at C-5 of two of them being undetermined, we have synthesized the four possible pentasaccharides, and have evaluated their FGF-2 bindi...

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Detalles Bibliográficos
Autor principal: Kovensky, José Eduardo
Publicado: 1999
Materias:
Carbohydrates
Glycosylation
Growth factors
Heparan sulfate
fibroblast growth factor 2
heparan sulfate
pentasaccharide
aorta
article
binding affinity
binding site
chemical structure
controlled study
human
human cell
intestine
synthesis
Aorta
Binding Sites
Carbohydrate Sequence
Cell Division
Cells, Cultured
Fibroblast Growth Factor 2
Glucuronic Acid
Heparitin Sulfate
Humans
Iduronic Acid
Isomerism
Molecular Sequence Data
Muscle, Smooth, Vascular
Oligosaccharides
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09680896_v7_n8_p1567_Kovensky
http://hdl.handle.net/20.500.12110/paper_09680896_v7_n8_p1567_Kovensky
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:It has been suggested that the FGF-2 binding site on heparan sulfate chains is a trisulfated pentasaccharide containing three hexuronic acid units. The configuration at C-5 of two of them being undetermined, we have synthesized the four possible pentasaccharides, and have evaluated their FGF-2 binding affinity through in vitro biological assays. The pentasaccharide containing L-iduronic acid as the sole hexuronic acid showed higher affinity for FGF-2 than the other pentasaccharides, where one hexuronic acid unit at least is D-glucuronic acid. Copyright (C) 1999.

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