A synthetic heparan sulfate pentasaccharide, exclusively containing L-iduronic acid, displays higher affinity for FGF-2 than its D-glucuronic acid-containing isomers
It has been suggested that the FGF-2 binding site on heparan sulfate chains is a trisulfated pentasaccharide containing three hexuronic acid units. The configuration at C-5 of two of them being undetermined, we have synthesized the four possible pentasaccharides, and have evaluated their FGF-2 bindi...
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1999
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09680896_v7_n8_p1567_Kovensky http://hdl.handle.net/20.500.12110/paper_09680896_v7_n8_p1567_Kovensky |
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paper:paper_09680896_v7_n8_p1567_Kovensky2023-06-08T15:58:59Z A synthetic heparan sulfate pentasaccharide, exclusively containing L-iduronic acid, displays higher affinity for FGF-2 than its D-glucuronic acid-containing isomers Kovensky, José Eduardo Carbohydrates Glycosylation Growth factors Heparan sulfate fibroblast growth factor 2 heparan sulfate pentasaccharide aorta article binding affinity binding site chemical structure controlled study human human cell intestine synthesis Aorta Binding Sites Carbohydrate Sequence Cell Division Cells, Cultured Fibroblast Growth Factor 2 Glucuronic Acid Heparitin Sulfate Humans Iduronic Acid Isomerism Molecular Sequence Data Muscle, Smooth, Vascular Oligosaccharides It has been suggested that the FGF-2 binding site on heparan sulfate chains is a trisulfated pentasaccharide containing three hexuronic acid units. The configuration at C-5 of two of them being undetermined, we have synthesized the four possible pentasaccharides, and have evaluated their FGF-2 binding affinity through in vitro biological assays. The pentasaccharide containing L-iduronic acid as the sole hexuronic acid showed higher affinity for FGF-2 than the other pentasaccharides, where one hexuronic acid unit at least is D-glucuronic acid. Copyright (C) 1999. Fil:Kovensky, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09680896_v7_n8_p1567_Kovensky http://hdl.handle.net/20.500.12110/paper_09680896_v7_n8_p1567_Kovensky |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Carbohydrates Glycosylation Growth factors Heparan sulfate fibroblast growth factor 2 heparan sulfate pentasaccharide aorta article binding affinity binding site chemical structure controlled study human human cell intestine synthesis Aorta Binding Sites Carbohydrate Sequence Cell Division Cells, Cultured Fibroblast Growth Factor 2 Glucuronic Acid Heparitin Sulfate Humans Iduronic Acid Isomerism Molecular Sequence Data Muscle, Smooth, Vascular Oligosaccharides |
| spellingShingle |
Carbohydrates Glycosylation Growth factors Heparan sulfate fibroblast growth factor 2 heparan sulfate pentasaccharide aorta article binding affinity binding site chemical structure controlled study human human cell intestine synthesis Aorta Binding Sites Carbohydrate Sequence Cell Division Cells, Cultured Fibroblast Growth Factor 2 Glucuronic Acid Heparitin Sulfate Humans Iduronic Acid Isomerism Molecular Sequence Data Muscle, Smooth, Vascular Oligosaccharides Kovensky, José Eduardo A synthetic heparan sulfate pentasaccharide, exclusively containing L-iduronic acid, displays higher affinity for FGF-2 than its D-glucuronic acid-containing isomers |
| topic_facet |
Carbohydrates Glycosylation Growth factors Heparan sulfate fibroblast growth factor 2 heparan sulfate pentasaccharide aorta article binding affinity binding site chemical structure controlled study human human cell intestine synthesis Aorta Binding Sites Carbohydrate Sequence Cell Division Cells, Cultured Fibroblast Growth Factor 2 Glucuronic Acid Heparitin Sulfate Humans Iduronic Acid Isomerism Molecular Sequence Data Muscle, Smooth, Vascular Oligosaccharides |
| description |
It has been suggested that the FGF-2 binding site on heparan sulfate chains is a trisulfated pentasaccharide containing three hexuronic acid units. The configuration at C-5 of two of them being undetermined, we have synthesized the four possible pentasaccharides, and have evaluated their FGF-2 binding affinity through in vitro biological assays. The pentasaccharide containing L-iduronic acid as the sole hexuronic acid showed higher affinity for FGF-2 than the other pentasaccharides, where one hexuronic acid unit at least is D-glucuronic acid. Copyright (C) 1999. |
| author |
Kovensky, José Eduardo |
| author_facet |
Kovensky, José Eduardo |
| author_sort |
Kovensky, José Eduardo |
| title |
A synthetic heparan sulfate pentasaccharide, exclusively containing L-iduronic acid, displays higher affinity for FGF-2 than its D-glucuronic acid-containing isomers |
| title_short |
A synthetic heparan sulfate pentasaccharide, exclusively containing L-iduronic acid, displays higher affinity for FGF-2 than its D-glucuronic acid-containing isomers |
| title_full |
A synthetic heparan sulfate pentasaccharide, exclusively containing L-iduronic acid, displays higher affinity for FGF-2 than its D-glucuronic acid-containing isomers |
| title_fullStr |
A synthetic heparan sulfate pentasaccharide, exclusively containing L-iduronic acid, displays higher affinity for FGF-2 than its D-glucuronic acid-containing isomers |
| title_full_unstemmed |
A synthetic heparan sulfate pentasaccharide, exclusively containing L-iduronic acid, displays higher affinity for FGF-2 than its D-glucuronic acid-containing isomers |
| title_sort |
synthetic heparan sulfate pentasaccharide, exclusively containing l-iduronic acid, displays higher affinity for fgf-2 than its d-glucuronic acid-containing isomers |
| publishDate |
1999 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09680896_v7_n8_p1567_Kovensky http://hdl.handle.net/20.500.12110/paper_09680896_v7_n8_p1567_Kovensky |
| work_keys_str_mv |
AT kovenskyjoseeduardo asyntheticheparansulfatepentasaccharideexclusivelycontainingliduronicaciddisplayshigheraffinityforfgf2thanitsdglucuronicacidcontainingisomers AT kovenskyjoseeduardo syntheticheparansulfatepentasaccharideexclusivelycontainingliduronicaciddisplayshigheraffinityforfgf2thanitsdglucuronicacidcontainingisomers |
| _version_ |
1768546684907814912 |