Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)

Cyanobacteria produce a wide array of substances with activity in many biological systems. The aim of the present research was to compare the effect of differently treated extracellular products (EP) from Tolypothrix tenuis (Cyanobacteria) on rice plantlet regeneration as well as on the pigments, pr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zaccaro, María Cristina, Stella de Rosellini, Ana María Cristina
Publicado:	2003
Materias:	Bioactive extracellular substances Cyanobacteria Plant growth regulators Plant regeneration Porphyrin Rice callus Tolypothrix tenuis Calothrix sp. PCC 7101 Tolypothrix
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v19_n1_p29_DeCano http://hdl.handle.net/20.500.12110/paper_09593993_v19_n1_p29_DeCano
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_09593993_v19_n1_p29_DeCano
record_format	dspace
spelling	paper:paper_09593993_v19_n1_p29_DeCano2023-06-08T15:56:51Z Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria) Zaccaro, María Cristina Stella de Rosellini, Ana María Cristina Bioactive extracellular substances Cyanobacteria Plant growth regulators Plant regeneration Porphyrin Rice callus Tolypothrix tenuis Calothrix sp. PCC 7101 Cyanobacteria Tolypothrix Cyanobacteria produce a wide array of substances with activity in many biological systems. The aim of the present research was to compare the effect of differently treated extracellular products (EP) from Tolypothrix tenuis (Cyanobacteria) on rice plantlet regeneration as well as on the pigments, protein, total free porphyrin contents, and 5-aminolevulinate dehydratase (ALA-D) activity in rice callus during differentiation. Rice embryo calli were regenerated in Murashige & Skoog medium supplemented with EP with protein (EPp) or without protein (EPnp) or autoclaved (EPa), as well as with benzyladenine (BA) and benzyladenine + naphthaleneacetic acid (BA + NAA). At day 75, calli percentage regeneration were: EPnp (84%), EPp (58%), BA + NAA (45%), BA (44%), EPa (40%). The same trend was found for chlorophyll a, b, total and carotenoid contents. Protein content in BA, BA + NAA and EPnp treatments was 35% higher than in EPp and EPa. Total free porphyrin was similar in all treatments. ALA-D activity in BA, EPp and EPa treatments was 28% higher than in BA + NAA and EPnp. The extracellular bioactive substance(s) from T. tenuis would contain a mixture of thermolabile plant growth regulators that replaced and improved the effects of synthetic plant growth regulators on rice callus organogenesis. Calli were rhizogenic in all the regeneration media tested. The pigment content of the calli was related to percentage regeneration but not to the total free porphyrin and ALA-D activity. Fil:Zaccaro, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stella, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v19_n1_p29_DeCano http://hdl.handle.net/20.500.12110/paper_09593993_v19_n1_p29_DeCano
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bioactive extracellular substances Cyanobacteria Plant growth regulators Plant regeneration Porphyrin Rice callus Tolypothrix tenuis Calothrix sp. PCC 7101 Cyanobacteria Tolypothrix
spellingShingle	Bioactive extracellular substances Cyanobacteria Plant growth regulators Plant regeneration Porphyrin Rice callus Tolypothrix tenuis Calothrix sp. PCC 7101 Cyanobacteria Tolypothrix Zaccaro, María Cristina Stella de Rosellini, Ana María Cristina Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)
topic_facet	Bioactive extracellular substances Cyanobacteria Plant growth regulators Plant regeneration Porphyrin Rice callus Tolypothrix tenuis Calothrix sp. PCC 7101 Cyanobacteria Tolypothrix
description	Cyanobacteria produce a wide array of substances with activity in many biological systems. The aim of the present research was to compare the effect of differently treated extracellular products (EP) from Tolypothrix tenuis (Cyanobacteria) on rice plantlet regeneration as well as on the pigments, protein, total free porphyrin contents, and 5-aminolevulinate dehydratase (ALA-D) activity in rice callus during differentiation. Rice embryo calli were regenerated in Murashige & Skoog medium supplemented with EP with protein (EPp) or without protein (EPnp) or autoclaved (EPa), as well as with benzyladenine (BA) and benzyladenine + naphthaleneacetic acid (BA + NAA). At day 75, calli percentage regeneration were: EPnp (84%), EPp (58%), BA + NAA (45%), BA (44%), EPa (40%). The same trend was found for chlorophyll a, b, total and carotenoid contents. Protein content in BA, BA + NAA and EPnp treatments was 35% higher than in EPp and EPa. Total free porphyrin was similar in all treatments. ALA-D activity in BA, EPp and EPa treatments was 28% higher than in BA + NAA and EPnp. The extracellular bioactive substance(s) from T. tenuis would contain a mixture of thermolabile plant growth regulators that replaced and improved the effects of synthetic plant growth regulators on rice callus organogenesis. Calli were rhizogenic in all the regeneration media tested. The pigment content of the calli was related to percentage regeneration but not to the total free porphyrin and ALA-D activity.
author	Zaccaro, María Cristina Stella de Rosellini, Ana María Cristina
author_facet	Zaccaro, María Cristina Stella de Rosellini, Ana María Cristina
author_sort	Zaccaro, María Cristina
title	Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)
title_short	Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)
title_full	Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)
title_fullStr	Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)
title_full_unstemmed	Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)
title_sort	enhancing rice callus regeneration by extracellular products of tolypothrix tenuis (cyanobacteria)
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v19_n1_p29_DeCano http://hdl.handle.net/20.500.12110/paper_09593993_v19_n1_p29_DeCano
work_keys_str_mv	AT zaccaromariacristina enhancingricecallusregenerationbyextracellularproductsoftolypothrixtenuiscyanobacteria AT stelladerosellinianamariacristina enhancingricecallusregenerationbyextracellularproductsoftolypothrixtenuiscyanobacteria
_version_	1768542700741591040

Enhancing rice callus regeneration by extracellular products of Tolypothrix tenuis (Cyanobacteria)

Ejemplares similares