The effect of griseofulvin on the heme pathway-II. An exhaustive analysis during short and long-term challenge
1. A clear biphasic response of the enzyme activities as a function of intoxication time due to the topical cutaneous griseofulvin treatment was observed. 2. The initial acute induction of ALA-S activity would be due to depletion of free heme in the regulatory pool caused by cytochrome P 450 destruc...
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1991
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03063623_v22_n6_p1179_Navone http://hdl.handle.net/20.500.12110/paper_03063623_v22_n6_p1179_Navone |
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paper:paper_03063623_v22_n6_p1179_Navone2023-06-08T15:31:05Z The effect of griseofulvin on the heme pathway-II. An exhaustive analysis during short and long-term challenge Buzaleh, Ana María Afonso, Susana Graciela Vázquez, Elba Susana Batlle, Alcira María del Carmen 5 aminolevulinate synthase griseofulvin porphobilinogen deaminase porphobilinogen synthase porphobilinogenase porphyrin unclassified drug uroporphyrinogen iii synthase animal experiment animal tissue article enzyme activity heme synthesis liver male metabolism mouse nonhuman oral drug administration porphyria priority journal tissue culture 5-Aminolevulinate Synthetase Ammonia-Lyases Animal Cell Nucleus Cytochrome P-450 Enzyme System Cytosol Griseofulvin Heme In Vitro Liver Male Mice Mitochondria, Liver Porphobilinogen Synthase Porphyrins Proteins Support, Non-U.S. Gov't 1. A clear biphasic response of the enzyme activities as a function of intoxication time due to the topical cutaneous griseofulvin treatment was observed. 2. The initial acute induction of ALA-S activity would be due to depletion of free heme in the regulatory pool caused by cytochrome P 450 destruction. 3. The second induction peak, would be due to less heme formation, secondary to the ferrochelatase inhibition, as expected for the erythropoietic protoporphyria model. 4. The biphasic response of hepatic ALA-D and PBGase activities would be related to ALA-S activity changes and the subsequent augmented available substrates. 5. Endogenous liver porphyrin distribution in cytosolic, mitochondrial and nuclear fractions was investigated. 6. The in vitro biosynthesis of porphyrins confirmed both the biphasic model and the hepatic porphyrins subcellular distribution. 7. Two mechanisms to explain the action of griseofulvin at shorter and longer times of intoxication are proposed. © 1991. Fil:Buzaleh, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Afonso, S.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vázquez, E.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1991 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03063623_v22_n6_p1179_Navone http://hdl.handle.net/20.500.12110/paper_03063623_v22_n6_p1179_Navone |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
5 aminolevulinate synthase griseofulvin porphobilinogen deaminase porphobilinogen synthase porphobilinogenase porphyrin unclassified drug uroporphyrinogen iii synthase animal experiment animal tissue article enzyme activity heme synthesis liver male metabolism mouse nonhuman oral drug administration porphyria priority journal tissue culture 5-Aminolevulinate Synthetase Ammonia-Lyases Animal Cell Nucleus Cytochrome P-450 Enzyme System Cytosol Griseofulvin Heme In Vitro Liver Male Mice Mitochondria, Liver Porphobilinogen Synthase Porphyrins Proteins Support, Non-U.S. Gov't |
| spellingShingle |
5 aminolevulinate synthase griseofulvin porphobilinogen deaminase porphobilinogen synthase porphobilinogenase porphyrin unclassified drug uroporphyrinogen iii synthase animal experiment animal tissue article enzyme activity heme synthesis liver male metabolism mouse nonhuman oral drug administration porphyria priority journal tissue culture 5-Aminolevulinate Synthetase Ammonia-Lyases Animal Cell Nucleus Cytochrome P-450 Enzyme System Cytosol Griseofulvin Heme In Vitro Liver Male Mice Mitochondria, Liver Porphobilinogen Synthase Porphyrins Proteins Support, Non-U.S. Gov't Buzaleh, Ana María Afonso, Susana Graciela Vázquez, Elba Susana Batlle, Alcira María del Carmen The effect of griseofulvin on the heme pathway-II. An exhaustive analysis during short and long-term challenge |
| topic_facet |
5 aminolevulinate synthase griseofulvin porphobilinogen deaminase porphobilinogen synthase porphobilinogenase porphyrin unclassified drug uroporphyrinogen iii synthase animal experiment animal tissue article enzyme activity heme synthesis liver male metabolism mouse nonhuman oral drug administration porphyria priority journal tissue culture 5-Aminolevulinate Synthetase Ammonia-Lyases Animal Cell Nucleus Cytochrome P-450 Enzyme System Cytosol Griseofulvin Heme In Vitro Liver Male Mice Mitochondria, Liver Porphobilinogen Synthase Porphyrins Proteins Support, Non-U.S. Gov't |
| description |
1. A clear biphasic response of the enzyme activities as a function of intoxication time due to the topical cutaneous griseofulvin treatment was observed. 2. The initial acute induction of ALA-S activity would be due to depletion of free heme in the regulatory pool caused by cytochrome P 450 destruction. 3. The second induction peak, would be due to less heme formation, secondary to the ferrochelatase inhibition, as expected for the erythropoietic protoporphyria model. 4. The biphasic response of hepatic ALA-D and PBGase activities would be related to ALA-S activity changes and the subsequent augmented available substrates. 5. Endogenous liver porphyrin distribution in cytosolic, mitochondrial and nuclear fractions was investigated. 6. The in vitro biosynthesis of porphyrins confirmed both the biphasic model and the hepatic porphyrins subcellular distribution. 7. Two mechanisms to explain the action of griseofulvin at shorter and longer times of intoxication are proposed. © 1991. |
| author |
Buzaleh, Ana María Afonso, Susana Graciela Vázquez, Elba Susana Batlle, Alcira María del Carmen |
| author_facet |
Buzaleh, Ana María Afonso, Susana Graciela Vázquez, Elba Susana Batlle, Alcira María del Carmen |
| author_sort |
Buzaleh, Ana María |
| title |
The effect of griseofulvin on the heme pathway-II. An exhaustive analysis during short and long-term challenge |
| title_short |
The effect of griseofulvin on the heme pathway-II. An exhaustive analysis during short and long-term challenge |
| title_full |
The effect of griseofulvin on the heme pathway-II. An exhaustive analysis during short and long-term challenge |
| title_fullStr |
The effect of griseofulvin on the heme pathway-II. An exhaustive analysis during short and long-term challenge |
| title_full_unstemmed |
The effect of griseofulvin on the heme pathway-II. An exhaustive analysis during short and long-term challenge |
| title_sort |
effect of griseofulvin on the heme pathway-ii. an exhaustive analysis during short and long-term challenge |
| publishDate |
1991 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03063623_v22_n6_p1179_Navone http://hdl.handle.net/20.500.12110/paper_03063623_v22_n6_p1179_Navone |
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