Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems

Whey protein concentrate (WPC) was fractionated by using hydroxypropylmethylcellulose (HPMC) at pH 6.5. Incompatible mixtures with different proportions of HPMC and WPC were prepared. After phase separation, the protein concentration in both phases was determined by the Kjeldahl method and the propo...

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Autores principales: Jara, Federico Luis, Pilosof, Ana María Renata
Publicado: 2011
Materias:
Hydroxypropylmethylcellulose
Phase separation
Protein partitioning
Thermodynamic incompatibility
Whey proteins
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v25_n3_p374_Jara
http://hdl.handle.net/20.500.12110/paper_0268005X_v25_n3_p374_Jara
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling paper:paper_0268005X_v25_n3_p374_Jara2025-07-30T18:01:57Z Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems Jara, Federico Luis Pilosof, Ana María Renata Hydroxypropylmethylcellulose Phase separation Protein partitioning Thermodynamic incompatibility Whey proteins Whey protein concentrate (WPC) was fractionated by using hydroxypropylmethylcellulose (HPMC) at pH 6.5. Incompatible mixtures with different proportions of HPMC and WPC were prepared. After phase separation, the protein concentration in both phases was determined by the Kjeldahl method and the proportion of each protein by SDS-PAGE combined with image analysis. The results show that the low molecular weight proteins α-lactalbumin (α-lac) and β-lactoglobulin (β-lg) were retained in high proportion in the upper phase (about 90% compared to 64% of WPC). The most efficient condition to fractionate β-lg and α-lac was the phase separation of an incompatible mixed system with a high initial concentration of WPC and a low initial concentration of HPMC i.e., WPC 20%, wt/wt/HPMC 0.5%, w/w. It can be concluded that the thermodynamic incompatibility which arises from mixing WPC with HPMC could be used as a method for fractionation of whey proteins. © 2010 Elsevier Ltd. Fil:Jara, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v25_n3_p374_Jara http://hdl.handle.net/20.500.12110/paper_0268005X_v25_n3_p374_Jara
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Hydroxypropylmethylcellulose
Phase separation
Protein partitioning
Thermodynamic incompatibility
Whey proteins
spellingShingle Hydroxypropylmethylcellulose
Phase separation
Protein partitioning
Thermodynamic incompatibility
Whey proteins
Jara, Federico Luis
Pilosof, Ana María Renata
Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems
topic_facet Hydroxypropylmethylcellulose
Phase separation
Protein partitioning
Thermodynamic incompatibility
Whey proteins
description Whey protein concentrate (WPC) was fractionated by using hydroxypropylmethylcellulose (HPMC) at pH 6.5. Incompatible mixtures with different proportions of HPMC and WPC were prepared. After phase separation, the protein concentration in both phases was determined by the Kjeldahl method and the proportion of each protein by SDS-PAGE combined with image analysis. The results show that the low molecular weight proteins α-lactalbumin (α-lac) and β-lactoglobulin (β-lg) were retained in high proportion in the upper phase (about 90% compared to 64% of WPC). The most efficient condition to fractionate β-lg and α-lac was the phase separation of an incompatible mixed system with a high initial concentration of WPC and a low initial concentration of HPMC i.e., WPC 20%, wt/wt/HPMC 0.5%, w/w. It can be concluded that the thermodynamic incompatibility which arises from mixing WPC with HPMC could be used as a method for fractionation of whey proteins. © 2010 Elsevier Ltd.
author Jara, Federico Luis
Pilosof, Ana María Renata
author_facet Jara, Federico Luis
Pilosof, Ana María Renata
author_sort Jara, Federico Luis
title Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems
title_short Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems
title_full Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems
title_fullStr Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems
title_full_unstemmed Partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems
title_sort partitioning of α-lactalbumin and β-lactoglobulin in whey protein concentrate/hydroxypropylmethylcellulose aqueous two-phase systems
publishDate 2011
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0268005X_v25_n3_p374_Jara
http://hdl.handle.net/20.500.12110/paper_0268005X_v25_n3_p374_Jara
work_keys_str_mv AT jarafedericoluis partitioningofalactalbuminandblactoglobulininwheyproteinconcentratehydroxypropylmethylcelluloseaqueoustwophasesystems
AT pilosofanamariarenata partitioningofalactalbuminandblactoglobulininwheyproteinconcentratehydroxypropylmethylcelluloseaqueoustwophasesystems
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