Aspergillus flavus dose-response curves to selected natural and synthetic antimicrobials
The effects of selected concentrations of antimicrobials from natural (vanillin, thymol, eugenol, carvacrol or citral) or synthetic (potassium sorbate or sodium benzoate) origin on Aspergillus flavus lag time inoculated in laboratory media formulated at water activity (aw) 0.99 and pH 4.5 or 3.5, we...
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2002
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681605_v73_n2-3_p213_LopezMalo http://hdl.handle.net/20.500.12110/paper_01681605_v73_n2-3_p213_LopezMalo |
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paper:paper_01681605_v73_n2-3_p213_LopezMalo2023-06-08T15:17:29Z Aspergillus flavus dose-response curves to selected natural and synthetic antimicrobials López-Malo Vigil, Aurelio Alzamora, Stella Maris Antimicrobials Aspergillus flavus Dose-response antiinfective agent benzoic acid carvacrol citral eugenol sorbate potassium thymol vanillin water antimicrobial activity article Aspergillus flavus concentration response controlled study drug effect drug response fungus growth growth inhibition minimum inhibitory concentration mould nonhuman pH Anti-Infective Agents Aspergillus flavus Colony Count, Microbial Dose-Response Relationship, Drug Food Preservatives Hydrogen-Ion Concentration Microbial Sensitivity Tests Sorbic Acid Thymol Time Factors Water Aspergillus Aspergillus flavus The effects of selected concentrations of antimicrobials from natural (vanillin, thymol, eugenol, carvacrol or citral) or synthetic (potassium sorbate or sodium benzoate) origin on Aspergillus flavus lag time inoculated in laboratory media formulated at water activity (aw) 0.99 and pH 4.5 or 3.5, were evaluated. Time to detect a colony with a diameter >0.5 mm was determined. Mold response was modeled using the Fermi function. Antimicrobial minimal inhibitory concentration (MIC) was defined as the minimal required inhibiting mold growth for 2 months. Fermi function successfully captured A. flavus dose-response curves to the tested antimicrobials with a highly satisfactory fit. Fermi equation coefficients, Pc and k, were used to compare antimicrobials and assess the effect of pH. Important differences in Pc and k were observed among antimicrobials, being natural antimicrobials less pH dependent than synthetic antimicrobials. A large Pc value represents a small antimicrobial effect on A. flavus lag time; thus, high concentrations are needed to delay growth. A. flavus exhibited higher sensitivity to thymol, eugenol, carvacrol, potassium sorbate (at pH 3.5), and sodium benzoate (at pH 3.5) than to vanillin or citral. MICs varied from 200 ppm of sodium benzoate at pH 3.5 to 1800 ppm of citral at both evaluated pHs. © 2002 Elsevier Science B.V. All rights reserved. Fil:López-Malo, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alzamora, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681605_v73_n2-3_p213_LopezMalo http://hdl.handle.net/20.500.12110/paper_01681605_v73_n2-3_p213_LopezMalo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antimicrobials Aspergillus flavus Dose-response antiinfective agent benzoic acid carvacrol citral eugenol sorbate potassium thymol vanillin water antimicrobial activity article Aspergillus flavus concentration response controlled study drug effect drug response fungus growth growth inhibition minimum inhibitory concentration mould nonhuman pH Anti-Infective Agents Aspergillus flavus Colony Count, Microbial Dose-Response Relationship, Drug Food Preservatives Hydrogen-Ion Concentration Microbial Sensitivity Tests Sorbic Acid Thymol Time Factors Water Aspergillus Aspergillus flavus |
| spellingShingle |
Antimicrobials Aspergillus flavus Dose-response antiinfective agent benzoic acid carvacrol citral eugenol sorbate potassium thymol vanillin water antimicrobial activity article Aspergillus flavus concentration response controlled study drug effect drug response fungus growth growth inhibition minimum inhibitory concentration mould nonhuman pH Anti-Infective Agents Aspergillus flavus Colony Count, Microbial Dose-Response Relationship, Drug Food Preservatives Hydrogen-Ion Concentration Microbial Sensitivity Tests Sorbic Acid Thymol Time Factors Water Aspergillus Aspergillus flavus López-Malo Vigil, Aurelio Alzamora, Stella Maris Aspergillus flavus dose-response curves to selected natural and synthetic antimicrobials |
| topic_facet |
Antimicrobials Aspergillus flavus Dose-response antiinfective agent benzoic acid carvacrol citral eugenol sorbate potassium thymol vanillin water antimicrobial activity article Aspergillus flavus concentration response controlled study drug effect drug response fungus growth growth inhibition minimum inhibitory concentration mould nonhuman pH Anti-Infective Agents Aspergillus flavus Colony Count, Microbial Dose-Response Relationship, Drug Food Preservatives Hydrogen-Ion Concentration Microbial Sensitivity Tests Sorbic Acid Thymol Time Factors Water Aspergillus Aspergillus flavus |
| description |
The effects of selected concentrations of antimicrobials from natural (vanillin, thymol, eugenol, carvacrol or citral) or synthetic (potassium sorbate or sodium benzoate) origin on Aspergillus flavus lag time inoculated in laboratory media formulated at water activity (aw) 0.99 and pH 4.5 or 3.5, were evaluated. Time to detect a colony with a diameter >0.5 mm was determined. Mold response was modeled using the Fermi function. Antimicrobial minimal inhibitory concentration (MIC) was defined as the minimal required inhibiting mold growth for 2 months. Fermi function successfully captured A. flavus dose-response curves to the tested antimicrobials with a highly satisfactory fit. Fermi equation coefficients, Pc and k, were used to compare antimicrobials and assess the effect of pH. Important differences in Pc and k were observed among antimicrobials, being natural antimicrobials less pH dependent than synthetic antimicrobials. A large Pc value represents a small antimicrobial effect on A. flavus lag time; thus, high concentrations are needed to delay growth. A. flavus exhibited higher sensitivity to thymol, eugenol, carvacrol, potassium sorbate (at pH 3.5), and sodium benzoate (at pH 3.5) than to vanillin or citral. MICs varied from 200 ppm of sodium benzoate at pH 3.5 to 1800 ppm of citral at both evaluated pHs. © 2002 Elsevier Science B.V. All rights reserved. |
| author |
López-Malo Vigil, Aurelio Alzamora, Stella Maris |
| author_facet |
López-Malo Vigil, Aurelio Alzamora, Stella Maris |
| author_sort |
López-Malo Vigil, Aurelio |
| title |
Aspergillus flavus dose-response curves to selected natural and synthetic antimicrobials |
| title_short |
Aspergillus flavus dose-response curves to selected natural and synthetic antimicrobials |
| title_full |
Aspergillus flavus dose-response curves to selected natural and synthetic antimicrobials |
| title_fullStr |
Aspergillus flavus dose-response curves to selected natural and synthetic antimicrobials |
| title_full_unstemmed |
Aspergillus flavus dose-response curves to selected natural and synthetic antimicrobials |
| title_sort |
aspergillus flavus dose-response curves to selected natural and synthetic antimicrobials |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681605_v73_n2-3_p213_LopezMalo http://hdl.handle.net/20.500.12110/paper_01681605_v73_n2-3_p213_LopezMalo |
| work_keys_str_mv |
AT lopezmalovigilaurelio aspergillusflavusdoseresponsecurvestoselectednaturalandsyntheticantimicrobials AT alzamorastellamaris aspergillusflavusdoseresponsecurvestoselectednaturalandsyntheticantimicrobials |
| _version_ |
1768545643657166848 |