Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G

The combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The m...

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Autor principal:	Briozzo, Jorge Emilio
Publicado:	1986
Materias:	botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v51_n4_p844_Briozzo http://hdl.handle.net/20.500.12110/paper_00992240_v51_n4_p844_Briozzo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00992240_v51_n4_p844_Briozzo
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spelling	paper:paper_00992240_v51_n4_p844_Briozzo2023-06-08T15:10:01Z Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G Briozzo, Jorge Emilio botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water The combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The minimum pH (at the optimum a(w)) for growth and toxin production of C. botulinum type G was found to be 5.6. Optimum conditions for toxin activation were a trypsin concentration of 0.1%, a pH of the medium of 6.5, and an incubation for 45 min at 37° C. These data did not show evidence of heat-labile spores, since a heat shock of 75°C for 10 min did not significantly decrease the spore count of strain 89G in media at pH 7.0 or 5.6. It was frequently observed that cells grown at reduced a(w) or pH experienced severe morphological changes. Fil:Briozzo, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1986 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v51_n4_p844_Briozzo http://hdl.handle.net/20.500.12110/paper_00992240_v51_n4_p844_Briozzo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water
spellingShingle	botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water Briozzo, Jorge Emilio Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
topic_facet	botulinum toxin water activity bacterial growth clostridium botulinum type g nonhuman ph priority journal Botulinum Toxins Clostridium botulinum Culture Media Heat Hydrogen-Ion Concentration Support, Non-U.S. Gov't Trypsin Water
description	The combined effect of water activity (a(w)) and pH on growth and toxin production by Clostridium botulinum type G strain 89 was investigated. The minimum a(w) at which growth and toxin formation occurred was 0.965, for media in which the pH was adjusted with either sodium chloride or sucrose. The minimum pH (at the optimum a(w)) for growth and toxin production of C. botulinum type G was found to be 5.6. Optimum conditions for toxin activation were a trypsin concentration of 0.1%, a pH of the medium of 6.5, and an incubation for 45 min at 37° C. These data did not show evidence of heat-labile spores, since a heat shock of 75°C for 10 min did not significantly decrease the spore count of strain 89G in media at pH 7.0 or 5.6. It was frequently observed that cells grown at reduced a(w) or pH experienced severe morphological changes.
author	Briozzo, Jorge Emilio
author_facet	Briozzo, Jorge Emilio
author_sort	Briozzo, Jorge Emilio
title	Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
title_short	Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
title_full	Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
title_fullStr	Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
title_full_unstemmed	Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G
title_sort	effect of water activity and ph on growth and toxin production by clostridium botulinum type g
publishDate	1986
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v51_n4_p844_Briozzo http://hdl.handle.net/20.500.12110/paper_00992240_v51_n4_p844_Briozzo
work_keys_str_mv	AT briozzojorgeemilio effectofwateractivityandphongrowthandtoxinproductionbyclostridiumbotulinumtypeg
_version_	1768542030170947584

Effect of water activity and pH on growth and toxin production by Clostridium botulinum type G

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