Cryopreservation of vanilla (Vanilla planifolia) root-tips : a new alternative for in vitro long-term storage of its germplasm

Vanilla planifolia (Orchidaceae) is the natural source of vanillin, which is the most widely appreciated flavor compound in the world. Although vanilla is cultivated throughout the tropics, their natural distribution areas are being severely reduced due to the anthropogenic impact. As a result, ex s...

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Detalles Bibliográficos
Autores principales: Dolce, Natalia Raquel, Hernández Ramírez, Fabiola, González Arnao, María Teresa
Formato: Documento de conferencia
Lenguaje:Inglés
Publicado: Mahidol University. Faculty of Science. Department of Plant Science 2020
Materias:
Plant cryopreservation
Vanilla planifolia
Vanilla germplasm
Poster
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/9192
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-9192
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Plant cryopreservation
Vanilla planifolia
Vanilla germplasm
Poster
spellingShingle Plant cryopreservation
Vanilla planifolia
Vanilla germplasm
Poster
Dolce, Natalia Raquel
Hernández Ramírez, Fabiola
González Arnao, María Teresa
Cryopreservation of vanilla (Vanilla planifolia) root-tips : a new alternative for in vitro long-term storage of its germplasm
topic_facet Plant cryopreservation
Vanilla planifolia
Vanilla germplasm
Poster
description Vanilla planifolia (Orchidaceae) is the natural source of vanillin, which is the most widely appreciated flavor compound in the world. Although vanilla is cultivated throughout the tropics, their natural distribution areas are being severely reduced due to the anthropogenic impact. As a result, ex situ preservation actions are necessary to safeguard the threatened diversity of this species. Cryopreservation of vanilla shoot-tips has been previously reported using the droplet-vitrification technique. However, survival and plant regeneration following this protocol were low and little reproducible. In this study, we evaluated a new alternative for cryopreservation of vanilla germplasm by using root-tips as explants and following the droplet- vitrification protocol. Maximum survival (~60%) and further regeneration (~40%) were obtained by preconditioning root-tips isolated from in vitro propagated plants on semisolid MS with 0.3 M sucrose (1 day), exposing to loading solution consisting of 0.4 M sucrose + 2 M glycerol (30 min) followed by glycerol-sucrose plant vitrification solution PVS3 (60 min in ice), and direct plunging into liquid nitrogen in droplets of PVS3 placed on aluminum foils strips. Tissues were rewarmed by plunging the aluminum foils directly in liquid MS enriched with 1.2 M sucrose (15 min) at room temperature. rowth recovery and induction of buds were efficiently achieved by culturing cryostored root-tips on MS added with 1 mg L-1 KIN or BAP. Plant regeneration was achieved by transferring the induced buds to MS media. This protocol has great potential for long-term conservation of V. planifolia germplasm and of other vanilla relatives.
format Documento de conferencia
author Dolce, Natalia Raquel
Hernández Ramírez, Fabiola
González Arnao, María Teresa
author_facet Dolce, Natalia Raquel
Hernández Ramírez, Fabiola
González Arnao, María Teresa
author_sort Dolce, Natalia Raquel
title Cryopreservation of vanilla (Vanilla planifolia) root-tips : a new alternative for in vitro long-term storage of its germplasm
title_short Cryopreservation of vanilla (Vanilla planifolia) root-tips : a new alternative for in vitro long-term storage of its germplasm
title_full Cryopreservation of vanilla (Vanilla planifolia) root-tips : a new alternative for in vitro long-term storage of its germplasm
title_fullStr Cryopreservation of vanilla (Vanilla planifolia) root-tips : a new alternative for in vitro long-term storage of its germplasm
title_full_unstemmed Cryopreservation of vanilla (Vanilla planifolia) root-tips : a new alternative for in vitro long-term storage of its germplasm
title_sort cryopreservation of vanilla (vanilla planifolia) root-tips : a new alternative for in vitro long-term storage of its germplasm
publisher Mahidol University. Faculty of Science. Department of Plant Science
publishDate 2020
url http://repositorio.unne.edu.ar/handle/123456789/9192
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