Cryopreservation of Arachis Pintoi (leguminosae) Somatic Embryos

In this study, we successfully cryopreserved cotyledonary somatic embryos of diploid and triploid Arachis pintoi cytotypes using the encapsulation-dehydration technique. The highest survival rates were obtained when somatic embryos were encapsulated in calcium alginate beads and precultured in agita...

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Detalles Bibliográficos
Autores principales: Rey, Hebe Yolanda, Faloci, Mirta Mabel, Medina, Ricardo Daniel, Dolce, Natalia Raquel, Engelmann, Florent, Mroginski, Luis Amado
Formato: Artículo
Lenguaje:Inglés
Publicado: CryoLetters 2020
Materias:
Somatic embryo
Cryopreservation
Encapsulation-dehydration
Genetic stability
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/9159
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-9159
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Somatic embryo
Cryopreservation
Encapsulation-dehydration
Genetic stability
spellingShingle Somatic embryo
Cryopreservation
Encapsulation-dehydration
Genetic stability
Rey, Hebe Yolanda
Faloci, Mirta Mabel
Medina, Ricardo Daniel
Dolce, Natalia Raquel
Engelmann, Florent
Mroginski, Luis Amado
Cryopreservation of Arachis Pintoi (leguminosae) Somatic Embryos
topic_facet Somatic embryo
Cryopreservation
Encapsulation-dehydration
Genetic stability
description In this study, we successfully cryopreserved cotyledonary somatic embryos of diploid and triploid Arachis pintoi cytotypes using the encapsulation-dehydration technique. The highest survival rates were obtained when somatic embryos were encapsulated in calcium alginate beads and precultured in agitated (80 rpm) liquid establishment medium (EM) with daily increasing sucrose concentration (0.50, 0.75, and 1.0 M). The encapsulated somatic embryos were then dehydrated with silica gel for 5 h to 20% moisture content (fresh weight basis) and cooled either rapidly (direct immersion in liquid nitrogen, LN) or slowly (1ºC min-1 from 25ºC to -30ºC followed by immersion in LN). Beads were kept in LN for a minimum of 1 h and then were rapidly rewarmed in a 30ºC water-bath for 2 min. Finally, encapsulated somatic embryos were post-cultured in agitated (80 rpm) liquid EM with daily decreasing sucrose concentration (0.75 and 0.5 M) and transferred to solidified EM. Using this protocol, we obtained 26% and 30% plant regeneration from cryopreserved somatic embryos of diploid and triploid cytotypes. No morphological abnormalities were observed in any of the plants regenerated from cryopreserved embryos and their genetic stability was confirmed with 10 isozyme systems and nine RAPD profiles.
format Artículo
author Rey, Hebe Yolanda
Faloci, Mirta Mabel
Medina, Ricardo Daniel
Dolce, Natalia Raquel
Engelmann, Florent
Mroginski, Luis Amado
author_facet Rey, Hebe Yolanda
Faloci, Mirta Mabel
Medina, Ricardo Daniel
Dolce, Natalia Raquel
Engelmann, Florent
Mroginski, Luis Amado
author_sort Rey, Hebe Yolanda
title Cryopreservation of Arachis Pintoi (leguminosae) Somatic Embryos
title_short Cryopreservation of Arachis Pintoi (leguminosae) Somatic Embryos
title_full Cryopreservation of Arachis Pintoi (leguminosae) Somatic Embryos
title_fullStr Cryopreservation of Arachis Pintoi (leguminosae) Somatic Embryos
title_full_unstemmed Cryopreservation of Arachis Pintoi (leguminosae) Somatic Embryos
title_sort cryopreservation of arachis pintoi (leguminosae) somatic embryos
publisher CryoLetters
publishDate 2020
url http://repositorio.unne.edu.ar/handle/123456789/9159
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