Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)

Seeds of Ilex species are individually enclosed by a woody endocarp, forming a dispersal unit termed a pyrene. Embryos are undeveloped when fruits reach maturity. As a consequence, germination is low even after several months under proper conditions. In the present study, we present reliable and r...

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Autores principales: Dolce, Natalia Raquel, Medina, Ricardo Daniel, Mroginski, Luis Amado, Rey, Hebe Yolanda
Formato: Artículo
Lenguaje:Inglés
Publicado: Seed testing. Association International 2020
Materias:
Germination
Aquifoliaceae
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/9156
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RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
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spelling I48-R184-123456789-91562025-09-09T13:13:24Z Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae) Dolce, Natalia Raquel Medina, Ricardo Daniel Mroginski, Luis Amado Rey, Hebe Yolanda Germination Aquifoliaceae Seeds of Ilex species are individually enclosed by a woody endocarp, forming a dispersal unit termed a pyrene. Embryos are undeveloped when fruits reach maturity. As a consequence, germination is low even after several months under proper conditions. In the present study, we present reliable and reproducible techniques that enhance seed germination of Ilex brasiliensis, I. pseudoboxus and I. theezans by aseptically sowing whole pyrenes. The highest germination percentages (53-92%) were reached by in vitro culturing whole pyrenes on solidified (0.65% agar) quarter-strength Murashige and Skoog medium with 3% sucrose and 0.1 mg l-1 zeatin, and incubating in a growth room at 27 ± 2°C with a 14-hour photoperiod. Thus, the in vitro culture of whole pyrenes constitutes an easy and efficient technique to obtain quick germination of these Ilex species and may be an alternative to embryo rescue. Rapid progress in the application of this method to breeding and preservation programmes of Ilex species is expected. Fil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. Fil: Medina, Ricardo Daniel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. Fil: Rey, Hebe R. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. Fil: Rey, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. 2020-06-02T22:49:47Z 2020-06-02T22:49:47Z 2015-02 Artículo Dolce, Natalia Raquel, et al, 2015. Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae). Seed Science and technology. Estados Unidos: International Seed Testing Association, vol. 43, no 2, p.1-5. ISSN 1819-5717. 0251-0952 http://repositorio.unne.edu.ar/handle/123456789/9156 eng https://www.ingentaconnect.com/content/ista/sst/2015/00000043/00000002/art00013 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p.1-5 application/pdf Seed testing. Association International Seed Science and technology, vol. 43, no. 2, p.1-5.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Germination
Aquifoliaceae
spellingShingle Germination
Aquifoliaceae
Dolce, Natalia Raquel
Medina, Ricardo Daniel
Mroginski, Luis Amado
Rey, Hebe Yolanda
Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)
topic_facet Germination
Aquifoliaceae
description Seeds of Ilex species are individually enclosed by a woody endocarp, forming a dispersal unit termed a pyrene. Embryos are undeveloped when fruits reach maturity. As a consequence, germination is low even after several months under proper conditions. In the present study, we present reliable and reproducible techniques that enhance seed germination of Ilex brasiliensis, I. pseudoboxus and I. theezans by aseptically sowing whole pyrenes. The highest germination percentages (53-92%) were reached by in vitro culturing whole pyrenes on solidified (0.65% agar) quarter-strength Murashige and Skoog medium with 3% sucrose and 0.1 mg l-1 zeatin, and incubating in a growth room at 27 ± 2°C with a 14-hour photoperiod. Thus, the in vitro culture of whole pyrenes constitutes an easy and efficient technique to obtain quick germination of these Ilex species and may be an alternative to embryo rescue. Rapid progress in the application of this method to breeding and preservation programmes of Ilex species is expected.
format Artículo
author Dolce, Natalia Raquel
Medina, Ricardo Daniel
Mroginski, Luis Amado
Rey, Hebe Yolanda
author_facet Dolce, Natalia Raquel
Medina, Ricardo Daniel
Mroginski, Luis Amado
Rey, Hebe Yolanda
author_sort Dolce, Natalia Raquel
title Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)
title_short Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)
title_full Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)
title_fullStr Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)
title_full_unstemmed Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)
title_sort sowing pyrenes under aseptic conditions enhances seed germination of ilex brasi liensis, i. pseudoboxus and i. theezans (aquifoliaceae)
publisher Seed testing. Association International
publishDate 2020
url http://repositorio.unne.edu.ar/handle/123456789/9156
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