Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought

Reverse transcription of RNA followed by real-time quantitative PCR (qPCR) is to date the most reliable method for gene expression studies. However, to control the errors introduced along the numerous experimental procedures, it requires a normalization using internal reference genes with stable exp...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Acevedo, Raúl Maximiliano, Avico, Edgardo Hernán, Ruiz, Oscar Adolfo, Sansberro, Pedro Alfonso
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer 2020
Materias:
Abiotic stress
Gene expression
House-keeping genes
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/9149
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-9149
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Abiotic stress
Gene expression
House-keeping genes
spellingShingle Abiotic stress
Gene expression
House-keeping genes
Acevedo, Raúl Maximiliano
Avico, Edgardo Hernán
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought
topic_facet Abiotic stress
Gene expression
House-keeping genes
description Reverse transcription of RNA followed by real-time quantitative PCR (qPCR) is to date the most reliable method for gene expression studies. However, to control the errors introduced along the numerous experimental procedures, it requires a normalization using internal reference genes with stable expression. To address this issue, nine candidate reference genes were investigated in Ilex paraguariensis leaves subjected to water stress. To facilitate the selection, we analysed the real-time qPCR data with three different software programs. The obtained results support the conclusion that RNA polymerase associated protein rtf1 homolog (RTF) combined with any of the following pairs is the most suitable triad of genes to compute a normalization factor: elongation factor 1-alpha + tubulin alpha chain (EF1a + α-Tub), actin + cyclophilin 38 (ACT + CYP38), or cyclophilin 38 + vacuolar protein sorting-associated protein 18 homologs (CYP38 + VPS). Our analysis constitutes the first in-depth study to identify the appropriate reference genes for the quantification of transcription in Ilex paraguariensis leaves during drought and provides essential information for further gene expression studies in this tree species
format Artículo
author Acevedo, Raúl Maximiliano
Avico, Edgardo Hernán
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author_facet Acevedo, Raúl Maximiliano
Avico, Edgardo Hernán
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author_sort Acevedo, Raúl Maximiliano
title Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought
title_short Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought
title_full Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought
title_fullStr Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought
title_full_unstemmed Assessment of reference genes for real-time quantitative PCR normalization in Ilex paraguariensis leaves during drought
title_sort assessment of reference genes for real-time quantitative pcr normalization in ilex paraguariensis leaves during drought
publisher Springer
publishDate 2020
url http://repositorio.unne.edu.ar/handle/123456789/9149
work_keys_str_mv AT acevedoraulmaximiliano assessmentofreferencegenesforrealtimequantitativepcrnormalizationinilexparaguariensisleavesduringdrought
AT avicoedgardohernan assessmentofreferencegenesforrealtimequantitativepcrnormalizationinilexparaguariensisleavesduringdrought
AT ruizoscaradolfo assessmentofreferencegenesforrealtimequantitativepcrnormalizationinilexparaguariensisleavesduringdrought
AT sansberropedroalfonso assessmentofreferencegenesforrealtimequantitativepcrnormalizationinilexparaguariensisleavesduringdrought
bdutipo_str Repositorios
_version_ 1764820542339153920

Ejemplares similares