Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves

Ilex paraguariensis (yerba mate) is cultivated in the subtropical region of South America, where the occurrence of drought episodes limit yield. To explore the mechanisms that allow I. paraguariensis to overcome dehydration, we investigated (1) how gene expression varied between water-stressed and...

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Autores principales: Acevedo, Raúl Maximiliano, Avico, Edgardo Hernán, González, Sergio, Acácio Rodrígues, Salvador, Rivarola, Máximo, Paniego, Norma, Nunes Nesi, Adriano, Ruiz, Oscar Adolfo, Sansberro, Pedro Alfonso
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer Nature 2020
Materias:
Abiotic stress
De novo transcriptome
Drought stress
Cellular stress response
Rna-seq
Transcription factors
Yerba mate
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/9142
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-9142
record_format dspace
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Abiotic stress
De novo transcriptome
Drought stress
Cellular stress response
Rna-seq
Transcription factors
Yerba mate
spellingShingle Abiotic stress
De novo transcriptome
Drought stress
Cellular stress response
Rna-seq
Transcription factors
Yerba mate
Acevedo, Raúl Maximiliano
Avico, Edgardo Hernán
González, Sergio
Acácio Rodrígues, Salvador
Rivarola, Máximo
Paniego, Norma
Nunes Nesi, Adriano
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
topic_facet Abiotic stress
De novo transcriptome
Drought stress
Cellular stress response
Rna-seq
Transcription factors
Yerba mate
description Ilex paraguariensis (yerba mate) is cultivated in the subtropical region of South America, where the occurrence of drought episodes limit yield. To explore the mechanisms that allow I. paraguariensis to overcome dehydration, we investigated (1) how gene expression varied between water-stressed and non-stressed plants and (2) in what way the modulation of gene expression was linked to physiological status and metabolite composition. A total of 4920 differentially expressed transcripts were obtained through RNA-Seq after water deprivation. Drought induced the expression of several transcripts involved in the ABA-signalling pathway. Stomatal closure and leaf osmotic adjustments were promoted to minimize water loss, and these responses were accompanied by a high transcriptional remodeling of stress perception, signalling and transcriptional regulation, the photoprotective and antioxidant systems, and other stress-responsive genes. Simultaneously, significant changes in metabolite contents were detected. Glutamine, phenylalanine, isomaltose, fucose, and malate levels were shown to be positively correlated with dehydration. Principal component analysis showed differences in the metabolic profiles of control and stressed leaves. These results provide a comprehensive overview of how I. paraguariensis responds to dehydration at transcriptional and metabolomic levels and provide further characterization of the molecular mechanisms associated with drought response in perennial subtropical species.
format Artículo
author Acevedo, Raúl Maximiliano
Avico, Edgardo Hernán
González, Sergio
Acácio Rodrígues, Salvador
Rivarola, Máximo
Paniego, Norma
Nunes Nesi, Adriano
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author_facet Acevedo, Raúl Maximiliano
Avico, Edgardo Hernán
González, Sergio
Acácio Rodrígues, Salvador
Rivarola, Máximo
Paniego, Norma
Nunes Nesi, Adriano
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author_sort Acevedo, Raúl Maximiliano
title Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
title_short Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
title_full Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
title_fullStr Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
title_full_unstemmed Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves
title_sort transcript and metabolic adjustments triggered by drought in ilex paraguariensis leaves
publisher Springer Nature
publishDate 2020
url http://repositorio.unne.edu.ar/handle/123456789/9142
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