Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
To analyse the putative effects of putrescine on the plant response to salinity, micropropagated Lotus tenuis plants from wild-type and transgenic lines harbouring the pRD29A: oat arginine decarboxylase (ADC, EC 4.1.1.19) construct were subjected to a gradual increase in salinity that was appli...
Guardado en:
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer Science
2020
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| Materias: | |
| Acceso en línea: | http://repositorio.unne.edu.ar/handle/123456789/9120 |
| Aporte de: |
| Sumario: | To analyse the putative effects of putrescine on
the plant response to salinity, micropropagated Lotus tenuis
plants from wild-type and transgenic lines harbouring the
pRD29A: oat arginine decarboxylase (ADC, EC 4.1.1.19)
construct were subjected to a gradual increase in salinity
that was applied gradually by means of sodium chloride
irrigation (from 0 to 0.3 mol L−1) every 5 days up to the
maximum concentration. At the end of the experiment, the
transgenic lines were healthier than the wild-type plants
and displayed a smaller reduction in shoot biomass and
a slight increase in root growth in response to stress. The
overexpression of ADC increased osmotic adjustment (5.8-
fold) via the release of proline. The salinity treatment doubled
the potassium uptake by roots from transgenic ADC
stressed plants with a concomitant decrease in the accumulation
of sodium, balancing the Na+/
K+ ratio. Analysis of
gene expression, enzymatic activities and hormone metabolism
suggests a crosstalk between polyamines and abscisic
acid in response to salinity via modulation of the abscisic
acid biosynthesis-related enzyme 9-cis-epoxycarotenoid
dioxygenase (EC 1.13.11.51) at the transcriptional level. |
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