Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants

To analyse the putative effects of putrescine on the plant response to salinity, micropropagated Lotus tenuis plants from wild-type and transgenic lines harbouring the pRD29A: oat arginine decarboxylase (ADC, EC 4.1.1.19) construct were subjected to a gradual increase in salinity that was appli...

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Autores principales: Espasandin, Fabiana Daniela, Calzadilla, Pablo I., Maiale, Santiago Javier, Ruiz, Oscar Adolfo, Sansberro, Pedro Alfonso
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer Science 2020
Materias:
Putrescine
Abscisic acid
Nced
Salt tolerance
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/9120
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-9120
record_format dspace
spelling I48-R184-123456789-91202023-12-11T12:24:08Z Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants Espasandin, Fabiana Daniela Calzadilla, Pablo I. Maiale, Santiago Javier Ruiz, Oscar Adolfo Sansberro, Pedro Alfonso Putrescine Abscisic acid Nced Salt tolerance To analyse the putative effects of putrescine on the plant response to salinity, micropropagated Lotus tenuis plants from wild-type and transgenic lines harbouring the pRD29A: oat arginine decarboxylase (ADC, EC 4.1.1.19) construct were subjected to a gradual increase in salinity that was applied gradually by means of sodium chloride irrigation (from 0 to 0.3 mol L−1) every 5 days up to the maximum concentration. At the end of the experiment, the transgenic lines were healthier than the wild-type plants and displayed a smaller reduction in shoot biomass and a slight increase in root growth in response to stress. The overexpression of ADC increased osmotic adjustment (5.8- fold) via the release of proline. The salinity treatment doubled the potassium uptake by roots from transgenic ADC stressed plants with a concomitant decrease in the accumulation of sodium, balancing the Na+/ K+ ratio. Analysis of gene expression, enzymatic activities and hormone metabolism suggests a crosstalk between polyamines and abscisic acid in response to salinity via modulation of the abscisic acid biosynthesis-related enzyme 9-cis-epoxycarotenoid dioxygenase (EC 1.13.11.51) at the transcriptional level. Fil: Espasandin, Fabiana Daniela. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. Fil: Espasandin, Fabiana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. Fil: Calzadilla, Pablo I. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biotecnológicas; Argentina. Fil: Maiale, Santiago Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biotecnológicas; Argentina. Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Tecnológicas; Argentina. 2020-06-02T22:48:35Z 2020-06-02T22:48:35Z 2017-05-12 Artículo Espasandin, Fabiana D., et. al., 2018. Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants. J Plant Growth Regul. New York: Springer Science, no. 37, p. 156-165. ISSN 1435-8107. 0721-7595 http://repositorio.unne.edu.ar/handle/123456789/9120 eng https://link.springer.com/content/pdf/10.1007/s00344-017-9713-7.pdf https://link.springer.com/article/10.1007/s00344-017-9713-7 http://dx.doi.org/10.1007/s00344-017-9713-7 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 156-165 application/pdf Springer Science J Plant Growth Regul, 2018, no. 37, p. 156-165.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Putrescine
Abscisic acid
Nced
Salt tolerance
spellingShingle Putrescine
Abscisic acid
Nced
Salt tolerance
Espasandin, Fabiana Daniela
Calzadilla, Pablo I.
Maiale, Santiago Javier
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
topic_facet Putrescine
Abscisic acid
Nced
Salt tolerance
description To analyse the putative effects of putrescine on the plant response to salinity, micropropagated Lotus tenuis plants from wild-type and transgenic lines harbouring the pRD29A: oat arginine decarboxylase (ADC, EC 4.1.1.19) construct were subjected to a gradual increase in salinity that was applied gradually by means of sodium chloride irrigation (from 0 to 0.3 mol L−1) every 5 days up to the maximum concentration. At the end of the experiment, the transgenic lines were healthier than the wild-type plants and displayed a smaller reduction in shoot biomass and a slight increase in root growth in response to stress. The overexpression of ADC increased osmotic adjustment (5.8- fold) via the release of proline. The salinity treatment doubled the potassium uptake by roots from transgenic ADC stressed plants with a concomitant decrease in the accumulation of sodium, balancing the Na+/ K+ ratio. Analysis of gene expression, enzymatic activities and hormone metabolism suggests a crosstalk between polyamines and abscisic acid in response to salinity via modulation of the abscisic acid biosynthesis-related enzyme 9-cis-epoxycarotenoid dioxygenase (EC 1.13.11.51) at the transcriptional level.
format Artículo
author Espasandin, Fabiana Daniela
Calzadilla, Pablo I.
Maiale, Santiago Javier
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author_facet Espasandin, Fabiana Daniela
Calzadilla, Pablo I.
Maiale, Santiago Javier
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author_sort Espasandin, Fabiana Daniela
title Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
title_short Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
title_full Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
title_fullStr Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
title_full_unstemmed Overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
title_sort overexpression of the arginine decarboxylase gene improves tolerance to salt stress in lotus tenuis plants
publisher Springer Science
publishDate 2020
url http://repositorio.unne.edu.ar/handle/123456789/9120
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