Nanovenoms synthesis for its potential use in antisera production against rattlesnake venom (Crotalus durissus terrificus)
Snake antivenoms (AV) production involves successive inoculations of venom (V) in increasing form together with the addition of adjuvants (ADJ) that improve the immune response of animals destined for immunizations. On the other hand, nanoparticles (NP) are being studied with multiple purposes d...
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| Formato: | Reunión |
| Lenguaje: | Inglés |
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Sociedad Argentina de Investigación Clínica
2026
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| Acceso en línea: | http://repositorio.unne.edu.ar/handle/123456789/60057 |
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I48-R184-123456789-600572026-02-13T14:08:10Z Nanovenoms synthesis for its potential use in antisera production against rattlesnake venom (Crotalus durissus terrificus) Baudou, Federico Gastón Giorgi, Exequiel David Diaz, María Eugenia Fusco, Luciano Sebastián Municoy, Sofía Desimone, Martín Federico Leiva, Laura Cristina Ana De Marzi, Mauricio Cesar Crotalus durissus terrificus Venoms Antivenoms Snake antivenoms (AV) production involves successive inoculations of venom (V) in increasing form together with the addition of adjuvants (ADJ) that improve the immune response of animals destined for immunizations. On the other hand, nanoparticles (NP) are being studied with multiple purposes due to their potential therapeutic and immunomodulatory use, and for their ability to transport antigens and induce a specific response against it. Therefore, it is of interest to associate V to NP in order to replacing conventional ADJ in AV production. In this work, nanovenoms (NV) were synthesized by adsorption of Crotalus durissus terrificus (C.d.t.) V proteins with silica NP (NPsi) of 400 nm size (positive/negative charge, +/-). The NPsi (-) were synthesized according to the Stöber method, while a fraction was modified with APTES (NPsi +), and its load and size was verified by potential Z and DLS (dynamic light scattering) respectively. Then, 10 mg of NPsi +/- were incubated with 1 mg / ml of whole V in PBS for 3 h under stirring to favor proteins adsorption to the NPs surface. NV were photographed under MET and also a FTIR (Fourier Transform Infrared) was performed. Crotoxin presence, the main toxic component of C.d.t. venom, was analyzed by hemolysis radial test both in NV and supernatant. NV proteins desorbed by heat treatment were analyzed by SDS-PAGE and immunoblotting. NV FTIR spectra showed intermediate values between those that exhibited C.d.t. V and the NPsi separately. SDS-PAGE and immunoblotting tests confirmed the presence of proteins in NPsi particles and hemolytic halos demonstrated that NPsi +/- were capable of binding crotoxin molecules on their surface. The results reveal the presence of V in both types of NPsi, preserving its activity and therefore its native structure, evidences that allow progress in an upcoming study such as the evaluation of immunogenic activity in experimental animals. 2026-02-13T13:45:53Z 2026-02-13T13:45:53Z 2019 Reunión Baudou, Federico Gastón, et al., 2019. Nanovenoms synthesis for its potential use in antisera production against rattlesnake venom (Crotalus durissus terrificus). En: Reunión Anual de Sociedades de Biociencia 2019. LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica. LI Reunión Anual de la Asociación Argentina de Farmacología Experimental. XXI Reunión Anual de la Sociedad Argentina de Biología. XXXI Reunión Anual de la Sociedad Argentina de Protozoología. IX Reunión Anual de la Asociación Argentina de Nanomedicinas. VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio. Buenos Aires: Sociedad Argentina de Investigación Clínica; Sociedad de Farmacología Experimental; Sociedad Argentina de Biología; Sociedad Argentina de Protozoología; Asociación Argentina de Nanomedicinas; Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Histochemical Society, p. 283-283. 0025-7680 1669-9106 http://repositorio.unne.edu.ar/handle/123456789/60057 eng openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 283-283 application/pdf Sociedad Argentina de Investigación Clínica Sociedad de Farmacología Experimental Sociedad Argentina de Biología Sociedad Argentina de Protozoología Asociación Argentina de Nanomedicinas Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio Histochemical Society |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-184 |
| collection |
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) |
| language |
Inglés |
| topic |
Crotalus durissus terrificus Venoms Antivenoms |
| spellingShingle |
Crotalus durissus terrificus Venoms Antivenoms Baudou, Federico Gastón Giorgi, Exequiel David Diaz, María Eugenia Fusco, Luciano Sebastián Municoy, Sofía Desimone, Martín Federico Leiva, Laura Cristina Ana De Marzi, Mauricio Cesar Nanovenoms synthesis for its potential use in antisera production against rattlesnake venom (Crotalus durissus terrificus) |
| topic_facet |
Crotalus durissus terrificus Venoms Antivenoms |
| description |
Snake antivenoms (AV) production involves successive
inoculations of venom (V) in increasing form together with the
addition of adjuvants (ADJ) that improve the immune response of
animals destined for immunizations. On the other hand,
nanoparticles (NP) are being studied with multiple purposes due to
their potential therapeutic and immunomodulatory use, and for
their ability to transport antigens and induce a specific response
against it. Therefore, it is of interest to associate V to NP in order to
replacing conventional ADJ in AV production. In this work,
nanovenoms (NV) were synthesized by adsorption of Crotalus
durissus terrificus (C.d.t.) V proteins with silica NP (NPsi) of 400 nm
size (positive/negative charge, +/-). The NPsi (-) were synthesized
according to the Stöber method, while a fraction was modified with
APTES (NPsi +), and its load and size was verified by potential Z and
DLS (dynamic light scattering) respectively. Then, 10 mg of NPsi +/-
were incubated with 1 mg / ml of whole V in PBS for 3 h under
stirring to favor proteins adsorption to the NPs surface. NV were
photographed under MET and also a FTIR (Fourier Transform
Infrared) was performed. Crotoxin presence, the main toxic
component of C.d.t. venom, was analyzed by hemolysis radial test
both in NV and supernatant. NV proteins desorbed by heat
treatment were analyzed by SDS-PAGE and immunoblotting. NV
FTIR spectra showed intermediate values between those that
exhibited C.d.t. V and the NPsi separately. SDS-PAGE and
immunoblotting tests confirmed the presence of proteins in NPsi
particles and hemolytic halos demonstrated that NPsi +/- were
capable of binding crotoxin molecules on their surface. The results
reveal the presence of V in both types of NPsi, preserving its activity
and therefore its native structure, evidences that allow progress in
an upcoming study such as the evaluation of immunogenic activity
in experimental animals. |
| format |
Reunión |
| author |
Baudou, Federico Gastón Giorgi, Exequiel David Diaz, María Eugenia Fusco, Luciano Sebastián Municoy, Sofía Desimone, Martín Federico Leiva, Laura Cristina Ana De Marzi, Mauricio Cesar |
| author_facet |
Baudou, Federico Gastón Giorgi, Exequiel David Diaz, María Eugenia Fusco, Luciano Sebastián Municoy, Sofía Desimone, Martín Federico Leiva, Laura Cristina Ana De Marzi, Mauricio Cesar |
| author_sort |
Baudou, Federico Gastón |
| title |
Nanovenoms synthesis for its potential use in antisera production against rattlesnake venom (Crotalus durissus terrificus) |
| title_short |
Nanovenoms synthesis for its potential use in antisera production against rattlesnake venom (Crotalus durissus terrificus) |
| title_full |
Nanovenoms synthesis for its potential use in antisera production against rattlesnake venom (Crotalus durissus terrificus) |
| title_fullStr |
Nanovenoms synthesis for its potential use in antisera production against rattlesnake venom (Crotalus durissus terrificus) |
| title_full_unstemmed |
Nanovenoms synthesis for its potential use in antisera production against rattlesnake venom (Crotalus durissus terrificus) |
| title_sort |
nanovenoms synthesis for its potential use in antisera production against rattlesnake venom (crotalus durissus terrificus) |
| publisher |
Sociedad Argentina de Investigación Clínica |
| publishDate |
2026 |
| url |
http://repositorio.unne.edu.ar/handle/123456789/60057 |
| work_keys_str_mv |
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