Physicochemical and biological characterization of nanovenoms, a new tool formed by silica nanoparticles and Crotalus durissus terrificus venom

Nanoparticles (NPs) are being studied due to their potential use as therapeutic and immunomodulatory tools, including their ability to transport antigens with the aim to induce a specific immune response. The production of snake antivenoms (AV) involves several inoculations of venom (V) in the pre...

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Autores principales: Baudou, Federico Gastón, Fusco, Luciano Sebastián, Giorgi, Exequiel David, Diaz, María Eugenia, Municoyd, Sofía, Desimone, Martín Federico, Leiva, Laura Cristina Ana, De Marzi, Mauricio Cesar
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier B.V. 2026
Materias:
Silica nanoparticles
Nanovenoms
Venoms
Antivenoms
Adjuvants
Crotalus durissus terrificus
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/60043
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-60043
record_format dspace
spelling I48-R184-123456789-600432026-02-13T10:01:51Z Physicochemical and biological characterization of nanovenoms, a new tool formed by silica nanoparticles and Crotalus durissus terrificus venom Baudou, Federico Gastón Fusco, Luciano Sebastián Giorgi, Exequiel David Diaz, María Eugenia Municoyd, Sofía Desimone, Martín Federico Leiva, Laura Cristina Ana De Marzi, Mauricio Cesar Silica nanoparticles Nanovenoms Venoms Antivenoms Adjuvants Crotalus durissus terrificus Nanoparticles (NPs) are being studied due to their potential use as therapeutic and immunomodulatory tools, including their ability to transport antigens with the aim to induce a specific immune response. The production of snake antivenoms (AV) involves several inoculations of venom (V) in the presence of adjuvants (ADJ) to improve the immune response of inoculated animals, causing a decrease in its quality and shelf life. Therefore, it is interesting to develop new strategies for reduce these side effects. For that reason, associating V to NPs to replace conventional ADJ could be a useful tool for future AV production. In this work, nanovenoms (NVs) were generated by the adsorption of Crotalus durissus terrificus (Cdt) V proteins over silica NPs (SiNPs) synthesized according to the Stöber method. Microphotographies obtained under Transmission Electron Microscopy (TEM) displayed a protein crown over NPs and Fourier Transform Infrared (FT-IR) presented the expected spectra for NVs resulting from the sum of those exhibited by Cdt V and SiNPs separately. SDS PAGE and immunoblotting assays confirmed the presence of proteins over SiNPs. Furthermore, the different enzymatic activities detected demonstrated that SiNPs were capable of binding V proteins preserving its activity and therefore would keep its native structure. In the same way, the NVs conserve the potential cytotoxic effects present in the V as we observed when culturing THP-1 cells with these complexes. This evidence allows us to infer that developed NVs could be used as a new platform for the production of antisera or for immunomodulatory therapies. 2026-02-12T18:55:46Z 2026-02-12T18:55:46Z 2020 Artículo Baudou, Federico Gastón, et al., 2020. Physicochemical and biological characterization of nanovenoms, a new tool formed by silica nanoparticles and Crotalus durissus terrificus venom. Colloids and Surfaces B: Biointerfaces. Amsterdam: Elsevier B.V., vol. 193, p. 1-9. E-ISSN 1873-4367. http://repositorio.unne.edu.ar/handle/123456789/60043 eng https://doi.org/10.1016/j.colsurfb.2020.111128 closedAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 1-9 application/pdf Elsevier B.V. Colloids and Surfaces B: Biointerfaces, 2020, vol 193, p. 1-9.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Silica nanoparticles
Nanovenoms
Venoms
Antivenoms
Adjuvants
Crotalus durissus terrificus
spellingShingle Silica nanoparticles
Nanovenoms
Venoms
Antivenoms
Adjuvants
Crotalus durissus terrificus
Baudou, Federico Gastón
Fusco, Luciano Sebastián
Giorgi, Exequiel David
Diaz, María Eugenia
Municoyd, Sofía
Desimone, Martín Federico
Leiva, Laura Cristina Ana
De Marzi, Mauricio Cesar
Physicochemical and biological characterization of nanovenoms, a new tool formed by silica nanoparticles and Crotalus durissus terrificus venom
topic_facet Silica nanoparticles
Nanovenoms
Venoms
Antivenoms
Adjuvants
Crotalus durissus terrificus
description Nanoparticles (NPs) are being studied due to their potential use as therapeutic and immunomodulatory tools, including their ability to transport antigens with the aim to induce a specific immune response. The production of snake antivenoms (AV) involves several inoculations of venom (V) in the presence of adjuvants (ADJ) to improve the immune response of inoculated animals, causing a decrease in its quality and shelf life. Therefore, it is interesting to develop new strategies for reduce these side effects. For that reason, associating V to NPs to replace conventional ADJ could be a useful tool for future AV production. In this work, nanovenoms (NVs) were generated by the adsorption of Crotalus durissus terrificus (Cdt) V proteins over silica NPs (SiNPs) synthesized according to the Stöber method. Microphotographies obtained under Transmission Electron Microscopy (TEM) displayed a protein crown over NPs and Fourier Transform Infrared (FT-IR) presented the expected spectra for NVs resulting from the sum of those exhibited by Cdt V and SiNPs separately. SDS PAGE and immunoblotting assays confirmed the presence of proteins over SiNPs. Furthermore, the different enzymatic activities detected demonstrated that SiNPs were capable of binding V proteins preserving its activity and therefore would keep its native structure. In the same way, the NVs conserve the potential cytotoxic effects present in the V as we observed when culturing THP-1 cells with these complexes. This evidence allows us to infer that developed NVs could be used as a new platform for the production of antisera or for immunomodulatory therapies.
format Artículo
author Baudou, Federico Gastón
Fusco, Luciano Sebastián
Giorgi, Exequiel David
Diaz, María Eugenia
Municoyd, Sofía
Desimone, Martín Federico
Leiva, Laura Cristina Ana
De Marzi, Mauricio Cesar
author_facet Baudou, Federico Gastón
Fusco, Luciano Sebastián
Giorgi, Exequiel David
Diaz, María Eugenia
Municoyd, Sofía
Desimone, Martín Federico
Leiva, Laura Cristina Ana
De Marzi, Mauricio Cesar
author_sort Baudou, Federico Gastón
title Physicochemical and biological characterization of nanovenoms, a new tool formed by silica nanoparticles and Crotalus durissus terrificus venom
title_short Physicochemical and biological characterization of nanovenoms, a new tool formed by silica nanoparticles and Crotalus durissus terrificus venom
title_full Physicochemical and biological characterization of nanovenoms, a new tool formed by silica nanoparticles and Crotalus durissus terrificus venom
title_fullStr Physicochemical and biological characterization of nanovenoms, a new tool formed by silica nanoparticles and Crotalus durissus terrificus venom
title_full_unstemmed Physicochemical and biological characterization of nanovenoms, a new tool formed by silica nanoparticles and Crotalus durissus terrificus venom
title_sort physicochemical and biological characterization of nanovenoms, a new tool formed by silica nanoparticles and crotalus durissus terrificus venom
publisher Elsevier B.V.
publishDate 2026
url http://repositorio.unne.edu.ar/handle/123456789/60043
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