Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina

Bothrops snake venoms have been proved toxic to a variety of cell types, in both in vivo and in vitro models. Studies on the pharmacological actions of Bothrops venoms from Argentina are relatively scarce and the direct action of the crude venoms has not been assessed using cell culture models. In t...

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Autores principales: Bustillo, Soledad, Lucero, Raúl Horacio, Leiva, Laura Cristina, Acosta, Ofelia Cristina, Bal de Kier Joffé, Elisa Dora, Gorodner, Jorge Osvaldo
Formato: Artículo
Lenguaje:Inglés
Publicado: Universidad Estatal Paulista. Centro de Estudios de Venenos y Animales Ponzoñosos 2026
Materias:
Bothrops alternatus
Bothrops diporus
Cytotoxicity
C2C12
Apoptosis
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/60036
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-60036
record_format dspace
spelling I48-R184-123456789-600362026-02-12T12:53:43Z Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina Bustillo, Soledad Lucero, Raúl Horacio Leiva, Laura Cristina Acosta, Ofelia Cristina Bal de Kier Joffé, Elisa Dora Gorodner, Jorge Osvaldo Bothrops alternatus Bothrops diporus Cytotoxicity C2C12 Apoptosis Bothrops snake venoms have been proved toxic to a variety of cell types, in both in vivo and in vitro models. Studies on the pharmacological actions of Bothrops venoms from Argentina are relatively scarce and the direct action of the crude venoms has not been assessed using cell culture models. In this work, we investigated the cytotoxicity of crude venoms from B. alternatus and B. diporus in a skeletal muscle (C2C12) cell line, which is commonly used as a model for studying the myotoxic action of snake venom. Both venoms (1.25-50 µg/mL) induced an early and significant decrease in cell viability. The cytotoxic concentration 50 (CC50), determined three hours after exposure, revealed that B. diporus venom was significantly more cytotoxic (CC50: 2 µg/mL) than B. alternatus (CC50: 5.8 µg/mL). To investigate the cell death mechanism involved, myoblast cells were examined by phase contrast microscopy and after acridine orange and ethidium bromide fluorescence staining, respectively. Our data clearly demonstrated that an apoptotic mechanism mediated this cell line destruction. The current study aimed to provide new information on the cytotoxicity mechanisms of Argentine Bothrops snake venoms on a skeletal muscle cell line. 2026-02-12T12:13:31Z 2026-02-12T12:13:31Z 2009-02 Artículo Bustillo, Soledad, et al., 2009. Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina. Journal of Venomous Animals and Toxins including Tropical Diseases. São Paulo: Universidad Estatal Paulista. Centro de Estudios de Venenos y Animales Ponzoñosos, vol. 15, no. 1, p. 28-42. E-ISSN 1678-9199. DOI https://doi.org/10.1590/S1678-91992009000100004 1678-9180 http://repositorio.unne.edu.ar/handle/123456789/60036 eng https://doi.org/10.1590/S1678-91992009000100004 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 28-42 application/pdf Universidad Estatal Paulista. Centro de Estudios de Venenos y Animales Ponzoñosos Journal of Venomous Animals and Toxins including Tropical Diseases, 2009, vol. 15, no. 1, p. 28-42.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Bothrops alternatus
Bothrops diporus
Cytotoxicity
C2C12
Apoptosis
spellingShingle Bothrops alternatus
Bothrops diporus
Cytotoxicity
C2C12
Apoptosis
Bustillo, Soledad
Lucero, Raúl Horacio
Leiva, Laura Cristina
Acosta, Ofelia Cristina
Bal de Kier Joffé, Elisa Dora
Gorodner, Jorge Osvaldo
Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina
topic_facet Bothrops alternatus
Bothrops diporus
Cytotoxicity
C2C12
Apoptosis
description Bothrops snake venoms have been proved toxic to a variety of cell types, in both in vivo and in vitro models. Studies on the pharmacological actions of Bothrops venoms from Argentina are relatively scarce and the direct action of the crude venoms has not been assessed using cell culture models. In this work, we investigated the cytotoxicity of crude venoms from B. alternatus and B. diporus in a skeletal muscle (C2C12) cell line, which is commonly used as a model for studying the myotoxic action of snake venom. Both venoms (1.25-50 µg/mL) induced an early and significant decrease in cell viability. The cytotoxic concentration 50 (CC50), determined three hours after exposure, revealed that B. diporus venom was significantly more cytotoxic (CC50: 2 µg/mL) than B. alternatus (CC50: 5.8 µg/mL). To investigate the cell death mechanism involved, myoblast cells were examined by phase contrast microscopy and after acridine orange and ethidium bromide fluorescence staining, respectively. Our data clearly demonstrated that an apoptotic mechanism mediated this cell line destruction. The current study aimed to provide new information on the cytotoxicity mechanisms of Argentine Bothrops snake venoms on a skeletal muscle cell line.
format Artículo
author Bustillo, Soledad
Lucero, Raúl Horacio
Leiva, Laura Cristina
Acosta, Ofelia Cristina
Bal de Kier Joffé, Elisa Dora
Gorodner, Jorge Osvaldo
author_facet Bustillo, Soledad
Lucero, Raúl Horacio
Leiva, Laura Cristina
Acosta, Ofelia Cristina
Bal de Kier Joffé, Elisa Dora
Gorodner, Jorge Osvaldo
author_sort Bustillo, Soledad
title Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina
title_short Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina
title_full Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina
title_fullStr Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina
title_full_unstemmed Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina
title_sort cytotoxicity and morphological analysis of cell death induced by bothrops venoms from the northeast of argentina
publisher Universidad Estatal Paulista. Centro de Estudios de Venenos y Animales Ponzoñosos
publishDate 2026
url http://repositorio.unne.edu.ar/handle/123456789/60036
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