Systemic pathological alterations caused by Philodryas patagoniensis colubrid snake venom in rats

Very little is known about the systemic effects caused by Philodryas patagoniensis colubrid snake venom. In this work, this venom was tested for its ability to induce histopathological changes in rats after its intramuscular, subcutaneous, or intravenous administration, by light microscopic examinat...

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Autores principales: Peichoto, María Elisa, Teibler, Gladys Pamela, Ruiz, Raquel Mónica, Leiva, Laura Cristina Ana, Acosta, Ofelia Cristina
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2025
Materias:
Colubridae
Philodryas patagoniensis
Venom
Systemic histopathological changes
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/59447
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-59447
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spelling I48-R184-123456789-594472025-12-16T14:23:16Z Systemic pathological alterations caused by Philodryas patagoniensis colubrid snake venom in rats Peichoto, María Elisa Teibler, Gladys Pamela Ruiz, Raquel Mónica Leiva, Laura Cristina Ana Acosta, Ofelia Cristina Colubridae Philodryas patagoniensis Venom Systemic histopathological changes Very little is known about the systemic effects caused by Philodryas patagoniensis colubrid snake venom. In this work, this venom was tested for its ability to induce histopathological changes in rats after its intramuscular, subcutaneous, or intravenous administration, by light microscopic examination of some organs (cerebellum, cerebrum, lung, liver, kidney, and heart). Four rats were used for each dose of 0.23, 0.45, and 0.90 mg of venom in 0.3 ml of phosphate-buffered saline solution (pH 7.4). Aliquots of blood were withdrawn at different time intervals for enzymatic determination of alanine aminotransferase, aspartate aminotransferase, and creatine kinase levels. After 2 h the animals were killed by an overdose of anesthetic, and samples of kidney, heart, liver, lung, cerebrum, and cerebellum were taken for microscopic examination (hematoxylin and eosin stain). Histologically, no abnormality was observed in heart tissue in any of the administration routes of the venom used. However, histological observations showed multifocal hemorrhage in cerebellum, cerebrum, and lung sections; severe peritubular capillary congestion in kidney sections; and hydropic degeneration in liver sections when venom was administered intravenously. The subcutaneous route showed similar results to the previous one, with the exception of cerebellar hemorrhage. Intramuscularly, neither cerebral nor cerebellar hemorrhage was observed. Plasma alanine aminotransferase and aspartate aminotransferase increased levels were demonstrated, mainly when venom was administered intravenously or subcutaneously. Our results suggest that P. patagoniensis venom induces moderate histopathological changes in vital organs of rats. These changes are initiated at early stages of the envenomation and may be associated with a behavioral or functional abnormality of those organs during envenoming. 2025-12-15T11:37:33Z 2025-12-15T11:37:33Z 2006-10 Artículo Peichoto, María Elisa, et al., 2006. Systemic pathological alterations caused by Philodryas patagoniensis colubrid snake venom in rats. Toxicon. Oxford: Elsevier, vol. 48, no. 5, p. 520-528. E-ISSN 1879-3150. 0041-0101 http://repositorio.unne.edu.ar/handle/123456789/59447 eng https://doi.org/10.1016/j.toxicon.2006.06.013 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 520-528 application/pdf Elsevier Toxicon, 2006, vol. 48, no. 5, p. 520-528.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Colubridae
Philodryas patagoniensis
Venom
Systemic histopathological changes
spellingShingle Colubridae
Philodryas patagoniensis
Venom
Systemic histopathological changes
Peichoto, María Elisa
Teibler, Gladys Pamela
Ruiz, Raquel Mónica
Leiva, Laura Cristina Ana
Acosta, Ofelia Cristina
Systemic pathological alterations caused by Philodryas patagoniensis colubrid snake venom in rats
topic_facet Colubridae
Philodryas patagoniensis
Venom
Systemic histopathological changes
description Very little is known about the systemic effects caused by Philodryas patagoniensis colubrid snake venom. In this work, this venom was tested for its ability to induce histopathological changes in rats after its intramuscular, subcutaneous, or intravenous administration, by light microscopic examination of some organs (cerebellum, cerebrum, lung, liver, kidney, and heart). Four rats were used for each dose of 0.23, 0.45, and 0.90 mg of venom in 0.3 ml of phosphate-buffered saline solution (pH 7.4). Aliquots of blood were withdrawn at different time intervals for enzymatic determination of alanine aminotransferase, aspartate aminotransferase, and creatine kinase levels. After 2 h the animals were killed by an overdose of anesthetic, and samples of kidney, heart, liver, lung, cerebrum, and cerebellum were taken for microscopic examination (hematoxylin and eosin stain). Histologically, no abnormality was observed in heart tissue in any of the administration routes of the venom used. However, histological observations showed multifocal hemorrhage in cerebellum, cerebrum, and lung sections; severe peritubular capillary congestion in kidney sections; and hydropic degeneration in liver sections when venom was administered intravenously. The subcutaneous route showed similar results to the previous one, with the exception of cerebellar hemorrhage. Intramuscularly, neither cerebral nor cerebellar hemorrhage was observed. Plasma alanine aminotransferase and aspartate aminotransferase increased levels were demonstrated, mainly when venom was administered intravenously or subcutaneously. Our results suggest that P. patagoniensis venom induces moderate histopathological changes in vital organs of rats. These changes are initiated at early stages of the envenomation and may be associated with a behavioral or functional abnormality of those organs during envenoming.
format Artículo
author Peichoto, María Elisa
Teibler, Gladys Pamela
Ruiz, Raquel Mónica
Leiva, Laura Cristina Ana
Acosta, Ofelia Cristina
author_facet Peichoto, María Elisa
Teibler, Gladys Pamela
Ruiz, Raquel Mónica
Leiva, Laura Cristina Ana
Acosta, Ofelia Cristina
author_sort Peichoto, María Elisa
title Systemic pathological alterations caused by Philodryas patagoniensis colubrid snake venom in rats
title_short Systemic pathological alterations caused by Philodryas patagoniensis colubrid snake venom in rats
title_full Systemic pathological alterations caused by Philodryas patagoniensis colubrid snake venom in rats
title_fullStr Systemic pathological alterations caused by Philodryas patagoniensis colubrid snake venom in rats
title_full_unstemmed Systemic pathological alterations caused by Philodryas patagoniensis colubrid snake venom in rats
title_sort systemic pathological alterations caused by philodryas patagoniensis colubrid snake venom in rats
publisher Elsevier
publishDate 2025
url http://repositorio.unne.edu.ar/handle/123456789/59447
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