Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage

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Myotoxicity, one of the most relevant local manifestations in envenomation by Bothrops genus, may result from a direct action of myotoxins or be due to an indirect vascular degeneration and ischemia. Baltergin, a snake venom metalloproteinase (SVMP), isolated from Bothrops alternatus venom has be...

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Autores principales: Gay, Claudia Carolina, Maruñak, Silvana Licia, Teibler, Pamela, Leiva, Laura Cristina Ana, Acosta, Ofelia Cristina
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2025
Materias:
Baltergin
Bothrops alternatus
Hemorrhage
Myotoxicity
Polyclonal specific antibodies
SVMPs
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/59445
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-59445
record_format dspace
spelling I48-R184-123456789-594452025-12-16T14:58:45Z Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage Gay, Claudia Carolina Maruñak, Silvana Licia Teibler, Pamela Leiva, Laura Cristina Ana Acosta, Ofelia Cristina Baltergin Bothrops alternatus Hemorrhage Myotoxicity Polyclonal specific antibodies SVMPs Myotoxicity, one of the most relevant local manifestations in envenomation by Bothrops genus, may result from a direct action of myotoxins or be due to an indirect vascular degeneration and ischemia. Baltergin, a snake venom metalloproteinase (SVMP), isolated from Bothrops alternatus venom has been used to obtain monospecific IgG, in order to determine the relative role of toxin in myotoxicity induced by whole venom. Bothrops diporus venom, another medical relevant genus of the northeastern region of Argentina, was also studied. Anti-baltergin IgG was able to neutralize completely the hemorrhagic activity of B. alternatus venom at an antibodies:venom ratio of 30:1 (w:w). However, mice injected with B. diporus venom showed a small spot remaining even at the highest ratio of IgG:venom assayed (50:1; w:w). Specific antibodies were efficient to neutralize the myotoxicity of B. alternatus venom at ratio 30:1 (w:w) but did not neutralize the same effects in B. diporus venom. Anti-baltergin polyclonal antibodies were useful tools for revealing the central role of SVMPs in the development of myotoxicity of B. alternatus venom, as well as, helping to suggest indirectly presence of potent myotoxic phospholi- pases A2 (PLA2s) in B. diporus venom. 2025-12-15T11:37:32Z 2025-12-15T11:37:32Z 2013-03-03 Artículo Gay, Claudia Carolina, et al., 2013. Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage. Toxicon. Países Bajos: Elsevier, vol. 63, p. 104-111. E-ISSN 1879-3150. 0041-0101 http://repositorio.unne.edu.ar/handle/123456789/59445 eng http://dx.doi.org/10.1016/j.toxicon.2012.11.028 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 104-111 application/pdf Elsevier Toxicon, 2013, vol. 63, p. 104-111.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Baltergin
Bothrops alternatus
Hemorrhage
Myotoxicity
Polyclonal specific antibodies
SVMPs
spellingShingle Baltergin
Bothrops alternatus
Hemorrhage
Myotoxicity
Polyclonal specific antibodies
SVMPs
Gay, Claudia Carolina
Maruñak, Silvana Licia
Teibler, Pamela
Leiva, Laura Cristina Ana
Acosta, Ofelia Cristina
Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage
topic_facet Baltergin
Bothrops alternatus
Hemorrhage
Myotoxicity
Polyclonal specific antibodies
SVMPs
description Myotoxicity, one of the most relevant local manifestations in envenomation by Bothrops genus, may result from a direct action of myotoxins or be due to an indirect vascular degeneration and ischemia. Baltergin, a snake venom metalloproteinase (SVMP), isolated from Bothrops alternatus venom has been used to obtain monospecific IgG, in order to determine the relative role of toxin in myotoxicity induced by whole venom. Bothrops diporus venom, another medical relevant genus of the northeastern region of Argentina, was also studied. Anti-baltergin IgG was able to neutralize completely the hemorrhagic activity of B. alternatus venom at an antibodies:venom ratio of 30:1 (w:w). However, mice injected with B. diporus venom showed a small spot remaining even at the highest ratio of IgG:venom assayed (50:1; w:w). Specific antibodies were efficient to neutralize the myotoxicity of B. alternatus venom at ratio 30:1 (w:w) but did not neutralize the same effects in B. diporus venom. Anti-baltergin polyclonal antibodies were useful tools for revealing the central role of SVMPs in the development of myotoxicity of B. alternatus venom, as well as, helping to suggest indirectly presence of potent myotoxic phospholi- pases A2 (PLA2s) in B. diporus venom.
format Artículo
author Gay, Claudia Carolina
Maruñak, Silvana Licia
Teibler, Pamela
Leiva, Laura Cristina Ana
Acosta, Ofelia Cristina
author_facet Gay, Claudia Carolina
Maruñak, Silvana Licia
Teibler, Pamela
Leiva, Laura Cristina Ana
Acosta, Ofelia Cristina
author_sort Gay, Claudia Carolina
title Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage
title_short Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage
title_full Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage
title_fullStr Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage
title_full_unstemmed Effect of monospecific antibodies against baltergin in myotoxicity induced by Bothrops alternatus venom from northeast of Argentina. Role of metalloproteinases in muscle damage
title_sort effect of monospecific antibodies against baltergin in myotoxicity induced by bothrops alternatus venom from northeast of argentina. role of metalloproteinases in muscle damage
publisher Elsevier
publishDate 2025
url http://repositorio.unne.edu.ar/handle/123456789/59445
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