Breeding tetraploid Paspalum simplex : hybridization, early identification of apomicts, and impact of apomixis on hybrid performance

Apomictic grasses predominate among tropical forages, and specific breeding techniques are needed for their genetic improvement. The objectives of this study were (i) to generate hybrids by crossing tetraploid sexual and apomictic Paspalum simplex Morong genotypes, (ii) to develop a technique base...

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Autores principales: Brugnoli, Elsa Andrea, Martínez, Eric Javier, Ferrari Usandizaga, Silvana Consuelo, Zilli, Alex Leonel, Urbani, Mario Hugo, Acuña, Carlos Alberto
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Lenguaje:Inglés
Publicado: Crop Science Society of America 2025
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/59376
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spelling I48-R184-123456789-593762025-12-15T12:04:19Z Breeding tetraploid Paspalum simplex : hybridization, early identification of apomicts, and impact of apomixis on hybrid performance Brugnoli, Elsa Andrea Martínez, Eric Javier Ferrari Usandizaga, Silvana Consuelo Zilli, Alex Leonel Urbani, Mario Hugo Acuña, Carlos Alberto Apomictic grasses predominate among tropical forages, and specific breeding techniques are needed for their genetic improvement. The objectives of this study were (i) to generate hybrids by crossing tetraploid sexual and apomictic Paspalum simplex Morong genotypes, (ii) to develop a technique based on molecular markers for early identification of apomictic hybrids, and (iii) to determine the relationship between mode of reproduction and performance of hybrids. Crosses were made between two induced sexual and seven apomictic tetraploid plants. Identi- fication of apomictic hybrids during seedling stage was performed using a marker linked to apomixis, and by flow cytometric seed analysis. Hybrids were evaluated under field conditions for plant diameter and height, initial growth, and seasonal regrowth during two consecutive growing seasons. Sexual tetraploid plants used as female parents behaved as allogamous, since 95% of the progeny had male-specific markers. The ratio between apomictic and sexual hybrids differed from 1:8.7 to 1:0.6 among crosses, with a mean of 1:2.4. There was a 96% coincidence between the use of the apomixis-linked marker and flow cytometric seed analysis. A technique based on the use of early DNA isolation and the amplification of a molecular marker linked to apomixis was developed. We found no overall difference between apomictic and sexual hybrids for the evaluated traits, except for fall regrowth in the first year, for which apomictic hybrids were superior. Generation of large tetraploid hybrid progenies is possible in P. simplex. Segregation for mode of reproduction depends on parents involved. Agronomic performance is not related to reproductive mode among tetraploid hybrids. 2025-12-15T11:33:37Z 2025-12-15T11:33:37Z 2019-03 Artículo Brugnoli, Elsa Andrea, et al., 2019. Breeding tetraploid Paspalum simplex : hybridization, early identification of apomicts, and impact of apomixis on hybrid performance. Crop Science. Baltimore: Crop Science Society of America, vol. 59, no. 4, p. 1617-1624. E-ISSN0011-183X. 1435-0653 http://repositorio.unne.edu.ar/handle/123456789/59376 eng https://doi.org/10.2135/cropsci2018.12.0771 closedAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 1617-1624 application/pdf Crop Science Society of America Crop Science, 2019, vol. 59, no. 4, p. 1617-1624.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
description Apomictic grasses predominate among tropical forages, and specific breeding techniques are needed for their genetic improvement. The objectives of this study were (i) to generate hybrids by crossing tetraploid sexual and apomictic Paspalum simplex Morong genotypes, (ii) to develop a technique based on molecular markers for early identification of apomictic hybrids, and (iii) to determine the relationship between mode of reproduction and performance of hybrids. Crosses were made between two induced sexual and seven apomictic tetraploid plants. Identi- fication of apomictic hybrids during seedling stage was performed using a marker linked to apomixis, and by flow cytometric seed analysis. Hybrids were evaluated under field conditions for plant diameter and height, initial growth, and seasonal regrowth during two consecutive growing seasons. Sexual tetraploid plants used as female parents behaved as allogamous, since 95% of the progeny had male-specific markers. The ratio between apomictic and sexual hybrids differed from 1:8.7 to 1:0.6 among crosses, with a mean of 1:2.4. There was a 96% coincidence between the use of the apomixis-linked marker and flow cytometric seed analysis. A technique based on the use of early DNA isolation and the amplification of a molecular marker linked to apomixis was developed. We found no overall difference between apomictic and sexual hybrids for the evaluated traits, except for fall regrowth in the first year, for which apomictic hybrids were superior. Generation of large tetraploid hybrid progenies is possible in P. simplex. Segregation for mode of reproduction depends on parents involved. Agronomic performance is not related to reproductive mode among tetraploid hybrids.
format Artículo
author Brugnoli, Elsa Andrea
Martínez, Eric Javier
Ferrari Usandizaga, Silvana Consuelo
Zilli, Alex Leonel
Urbani, Mario Hugo
Acuña, Carlos Alberto
spellingShingle Brugnoli, Elsa Andrea
Martínez, Eric Javier
Ferrari Usandizaga, Silvana Consuelo
Zilli, Alex Leonel
Urbani, Mario Hugo
Acuña, Carlos Alberto
Breeding tetraploid Paspalum simplex : hybridization, early identification of apomicts, and impact of apomixis on hybrid performance
author_facet Brugnoli, Elsa Andrea
Martínez, Eric Javier
Ferrari Usandizaga, Silvana Consuelo
Zilli, Alex Leonel
Urbani, Mario Hugo
Acuña, Carlos Alberto
author_sort Brugnoli, Elsa Andrea
title Breeding tetraploid Paspalum simplex : hybridization, early identification of apomicts, and impact of apomixis on hybrid performance
title_short Breeding tetraploid Paspalum simplex : hybridization, early identification of apomicts, and impact of apomixis on hybrid performance
title_full Breeding tetraploid Paspalum simplex : hybridization, early identification of apomicts, and impact of apomixis on hybrid performance
title_fullStr Breeding tetraploid Paspalum simplex : hybridization, early identification of apomicts, and impact of apomixis on hybrid performance
title_full_unstemmed Breeding tetraploid Paspalum simplex : hybridization, early identification of apomicts, and impact of apomixis on hybrid performance
title_sort breeding tetraploid paspalum simplex : hybridization, early identification of apomicts, and impact of apomixis on hybrid performance
publisher Crop Science Society of America
publishDate 2025
url http://repositorio.unne.edu.ar/handle/123456789/59376
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