Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina

Mostrar todas las versiones(2)

The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in th...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Fusco, Luciano Sebastián, Neto, Emidio Beraldo, Francisco, Aleff Ferreira, Alfonso, Jorge, Soares, Andreimar, Pimenta, Daniel Carvalho, Leiva, Laura Cristina Ana
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2025
Materias:
Venomics
Crotalus durissus terrificus
Toxin families
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/59356
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-59356
record_format dspace
spelling I48-R184-123456789-593562025-12-18T12:20:32Z Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina Fusco, Luciano Sebastián Neto, Emidio Beraldo Francisco, Aleff Ferreira Alfonso, Jorge Soares, Andreimar Pimenta, Daniel Carvalho Leiva, Laura Cristina Ana Venomics Crotalus durissus terrificus Toxin families The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom’s composition—phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metalloprotease, phospholipase B and 5'-nucleotidase) and proteins (bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d. terrificus venom from Argentina. This proved to be crotamine-positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy. 2025-12-15T11:31:19Z 2025-12-15T11:31:19Z 2020-09 Artículo Fusco, Luciano Sebastián, et al., 2020. Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina. Toxicon: X. Oxford: Elsevier, vol. 7, p. 1-7. E-ISSN 1879-3150. 0041-0101 http://repositorio.unne.edu.ar/handle/123456789/59356 eng https://doi.org/10.1016/j.toxcx.2020.100047 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 1-7 application/pdf Elsevier Toxicon: X, 2020, vol. 7, p. 1-7.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Venomics
Crotalus durissus terrificus
Toxin families
spellingShingle Venomics
Crotalus durissus terrificus
Toxin families
Fusco, Luciano Sebastián
Neto, Emidio Beraldo
Francisco, Aleff Ferreira
Alfonso, Jorge
Soares, Andreimar
Pimenta, Daniel Carvalho
Leiva, Laura Cristina Ana
Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
topic_facet Venomics
Crotalus durissus terrificus
Toxin families
description The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom’s composition—phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metalloprotease, phospholipase B and 5'-nucleotidase) and proteins (bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d. terrificus venom from Argentina. This proved to be crotamine-positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy.
format Artículo
author Fusco, Luciano Sebastián
Neto, Emidio Beraldo
Francisco, Aleff Ferreira
Alfonso, Jorge
Soares, Andreimar
Pimenta, Daniel Carvalho
Leiva, Laura Cristina Ana
author_facet Fusco, Luciano Sebastián
Neto, Emidio Beraldo
Francisco, Aleff Ferreira
Alfonso, Jorge
Soares, Andreimar
Pimenta, Daniel Carvalho
Leiva, Laura Cristina Ana
author_sort Fusco, Luciano Sebastián
title Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_short Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_full Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_fullStr Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_full_unstemmed Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_sort fast venomic analysis of crotalus durissus terrificus from northeastern argentina
publisher Elsevier
publishDate 2025
url http://repositorio.unne.edu.ar/handle/123456789/59356
work_keys_str_mv AT fuscolucianosebastian fastvenomicanalysisofcrotalusdurissusterrificusfromnortheasternargentina
AT netoemidioberaldo fastvenomicanalysisofcrotalusdurissusterrificusfromnortheasternargentina
AT franciscoaleffferreira fastvenomicanalysisofcrotalusdurissusterrificusfromnortheasternargentina
AT alfonsojorge fastvenomicanalysisofcrotalusdurissusterrificusfromnortheasternargentina
AT soaresandreimar fastvenomicanalysisofcrotalusdurissusterrificusfromnortheasternargentina
AT pimentadanielcarvalho fastvenomicanalysisofcrotalusdurissusterrificusfromnortheasternargentina
AT leivalauracristinaana fastvenomicanalysisofcrotalusdurissusterrificusfromnortheasternargentina
_version_ 1857347449906003968

Ejemplares similares