Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells

The consumption of Ipomoea carnea produces a neurological syndrome in animals. The toxic principles of I. carnea are the alkaloids swainsonine (SW) and calystegines B1, B2, B3 and C1. In this study, we investigated the cytotoxicity of an alkaloid extract of Ipomoea carnea (AEE) and natural swainsoni...

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Autores principales: Cholich, Luciana, Pistan, María, Torres, Ana, Hernández, David, Moro, Ramiro, Gómez, Tamara, Gardner, Dale, Bustillo, Soledad
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2025
Materias:
Ipomoea carnea extract
Swainsonine
Cytotoxicity
Glioma
C6 cell line
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/59354
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-59354
record_format dspace
spelling I48-R184-123456789-593542025-12-15T12:01:49Z Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells Cholich, Luciana Pistan, María Torres, Ana Hernández, David Moro, Ramiro Gómez, Tamara Gardner, Dale Bustillo, Soledad Ipomoea carnea extract Swainsonine Cytotoxicity Glioma C6 cell line The consumption of Ipomoea carnea produces a neurological syndrome in animals. The toxic principles of I. carnea are the alkaloids swainsonine (SW) and calystegines B1, B2, B3 and C1. In this study, we investigated the cytotoxicity of an alkaloid extract of Ipomoea carnea (AEE) and natural swainsonine (SW) isolated from Astragalus lentiginosus (25–1000 μM of SW) for 48 h in a glioma cell line. Although the natural SW did not induce any changes in cell viability, the AEE exhibited a dose dependent cytotoxic effect and release of lactate dehydrogenase (LDH) indicative of cytolysis. In order to evaluate the morphological changes involved, cells were examined using phase contrast and fluorescence microscopy with acridine orange-ethidium bromide staining. The AEE caused a cell death compatible with necrosis, whereas exposure to 1000 μM of SW resulted in cytoplasmic vacuolation. Immunocytochemical studies revealed that astrocytes treated with 150 μM of AEE from I. carnea or 1000 μM of SW exhibited morphological characteristics of cell activation. These findings suggest that swainsonine would not be the only component present in the AEE of I. carnea responsible for in vitro cytotoxicity. Calystegines might also play a role in acting synergistically and triggering cell death through necrosis. 2025-12-15T11:31:18Z 2025-12-15T11:31:18Z 2023-11 Artículo Cholich, Luciana, et al., 2023. Ipomoea carnea alkaloid extract vs swainsonine: a comparative study on cytotoxic activity against glial cells. Toxicon. Oxford: Elsevier, vol. 235, p. 107325. ISSN 1879-3150 0041-0101 http://repositorio.unne.edu.ar/handle/123456789/59354 eng https://doi.org/10.1016/j.toxicon.2023.107325 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 107325 application/pdf Elsevier Toxicon, 2023, vol. 235, p. 107325.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Ipomoea carnea extract
Swainsonine
Cytotoxicity
Glioma
C6 cell line
spellingShingle Ipomoea carnea extract
Swainsonine
Cytotoxicity
Glioma
C6 cell line
Cholich, Luciana
Pistan, María
Torres, Ana
Hernández, David
Moro, Ramiro
Gómez, Tamara
Gardner, Dale
Bustillo, Soledad
Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells
topic_facet Ipomoea carnea extract
Swainsonine
Cytotoxicity
Glioma
C6 cell line
description The consumption of Ipomoea carnea produces a neurological syndrome in animals. The toxic principles of I. carnea are the alkaloids swainsonine (SW) and calystegines B1, B2, B3 and C1. In this study, we investigated the cytotoxicity of an alkaloid extract of Ipomoea carnea (AEE) and natural swainsonine (SW) isolated from Astragalus lentiginosus (25–1000 μM of SW) for 48 h in a glioma cell line. Although the natural SW did not induce any changes in cell viability, the AEE exhibited a dose dependent cytotoxic effect and release of lactate dehydrogenase (LDH) indicative of cytolysis. In order to evaluate the morphological changes involved, cells were examined using phase contrast and fluorescence microscopy with acridine orange-ethidium bromide staining. The AEE caused a cell death compatible with necrosis, whereas exposure to 1000 μM of SW resulted in cytoplasmic vacuolation. Immunocytochemical studies revealed that astrocytes treated with 150 μM of AEE from I. carnea or 1000 μM of SW exhibited morphological characteristics of cell activation. These findings suggest that swainsonine would not be the only component present in the AEE of I. carnea responsible for in vitro cytotoxicity. Calystegines might also play a role in acting synergistically and triggering cell death through necrosis.
format Artículo
author Cholich, Luciana
Pistan, María
Torres, Ana
Hernández, David
Moro, Ramiro
Gómez, Tamara
Gardner, Dale
Bustillo, Soledad
author_facet Cholich, Luciana
Pistan, María
Torres, Ana
Hernández, David
Moro, Ramiro
Gómez, Tamara
Gardner, Dale
Bustillo, Soledad
author_sort Cholich, Luciana
title Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells
title_short Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells
title_full Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells
title_fullStr Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells
title_full_unstemmed Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells
title_sort ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells
publisher Elsevier
publishDate 2025
url http://repositorio.unne.edu.ar/handle/123456789/59354
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