Photobiomodulation therapy on local effects induced by juvenile and adult venoms of Bothrops alternatus

Bothrops snake envenomation is characterized by severe local manifestations such as pain, edema, inflammation, hemorrhage, and myonecrosis. Furthermore, it is described that venom from juvenile and adult snakes may have differences in their composition that can lead to differences in the evolution o...

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Autores principales: Cabral David, Amanda, Miato Gonçalves Silva, Luciana, Garcia Denegri, Maria Emília, Leiva, Laura Cristina Ana, Silva Junior, José Antonio, Pavan Zuliani, Juliana, Zamuner, Stella Regina
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2025
Materias:
Bothrops alternatus
Infammation
Myotoxicity
Photobiomodulation
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/59353
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-59353
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spelling I48-R184-123456789-593532025-12-15T12:01:42Z Photobiomodulation therapy on local effects induced by juvenile and adult venoms of Bothrops alternatus Cabral David, Amanda Miato Gonçalves Silva, Luciana Garcia Denegri, Maria Emília Leiva, Laura Cristina Ana Silva Junior, José Antonio Pavan Zuliani, Juliana Zamuner, Stella Regina Bothrops alternatus Infammation Myotoxicity Photobiomodulation Bothrops snake envenomation is characterized by severe local manifestations such as pain, edema, inflammation, hemorrhage, and myonecrosis. Furthermore, it is described that venom from juvenile and adult snakes may have differences in their composition that can lead to differences in the evolution of the clinical manifestation of the victim. Photobiomodulation (PBM) has been shown to be an effective adjuvant therapy to serum therapy to reduce the local effects induced by bothropic snake venom. This study evaluated the effect of PBM on the local reaction, after Bothrops alternatus snake venom (BaV) injection, in its juvenile (BaJV) and adult (BaAV) stages. Balb/C mice were injected with the juvenile or adult venoms of BaV or saline solution (control group). PBM at a wavelength of 660 nm, 100 mW, 0.33 W/cm², 40 s, and a 0.028 cm² beam was applied transcutaneous to a single point with a radiant exposure of 4 J/cm², 30 min after venom injection. Edema, inflammatory infiltrate, hyperalgesia, and myonecrosis were analyzed. Both venoms induced significant edema and myonecrosis in the gastrocnemius muscle. Hyperalgesia in the mice paw and a prominent leukocyte infiltrate into the peritoneum were also observed. PBM significantly reduced all evaluated parameters. In conclusion, PBM treatment was effective in reducing the local effects induced by B. alternatus venom at different stages of snake development and could be a useful tool as an adjuvant treatment for bothropic envenomation. 2025-12-15T11:31:18Z 2025-12-15T11:31:18Z 2022-12 Artículo Cabral David, Amanda, et al., 2022. Photobiomodulation therapy on local effects induced by juvenile and adult venoms of Bothrops alternatus. Toxicon. Oxford: Elsevier, vol. 220, 106941. ISSN 1879-3150 0041-0101 http://repositorio.unne.edu.ar/handle/123456789/59353 eng https://doi.org/10.1016/j.toxicon.2022.106941 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 106941 application/pdf Elsevier Toxicon, 2022, vol. 220, p. 106941.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Bothrops alternatus
Infammation
Myotoxicity
Photobiomodulation
spellingShingle Bothrops alternatus
Infammation
Myotoxicity
Photobiomodulation
Cabral David, Amanda
Miato Gonçalves Silva, Luciana
Garcia Denegri, Maria Emília
Leiva, Laura Cristina Ana
Silva Junior, José Antonio
Pavan Zuliani, Juliana
Zamuner, Stella Regina
Photobiomodulation therapy on local effects induced by juvenile and adult venoms of Bothrops alternatus
topic_facet Bothrops alternatus
Infammation
Myotoxicity
Photobiomodulation
description Bothrops snake envenomation is characterized by severe local manifestations such as pain, edema, inflammation, hemorrhage, and myonecrosis. Furthermore, it is described that venom from juvenile and adult snakes may have differences in their composition that can lead to differences in the evolution of the clinical manifestation of the victim. Photobiomodulation (PBM) has been shown to be an effective adjuvant therapy to serum therapy to reduce the local effects induced by bothropic snake venom. This study evaluated the effect of PBM on the local reaction, after Bothrops alternatus snake venom (BaV) injection, in its juvenile (BaJV) and adult (BaAV) stages. Balb/C mice were injected with the juvenile or adult venoms of BaV or saline solution (control group). PBM at a wavelength of 660 nm, 100 mW, 0.33 W/cm², 40 s, and a 0.028 cm² beam was applied transcutaneous to a single point with a radiant exposure of 4 J/cm², 30 min after venom injection. Edema, inflammatory infiltrate, hyperalgesia, and myonecrosis were analyzed. Both venoms induced significant edema and myonecrosis in the gastrocnemius muscle. Hyperalgesia in the mice paw and a prominent leukocyte infiltrate into the peritoneum were also observed. PBM significantly reduced all evaluated parameters. In conclusion, PBM treatment was effective in reducing the local effects induced by B. alternatus venom at different stages of snake development and could be a useful tool as an adjuvant treatment for bothropic envenomation.
format Artículo
author Cabral David, Amanda
Miato Gonçalves Silva, Luciana
Garcia Denegri, Maria Emília
Leiva, Laura Cristina Ana
Silva Junior, José Antonio
Pavan Zuliani, Juliana
Zamuner, Stella Regina
author_facet Cabral David, Amanda
Miato Gonçalves Silva, Luciana
Garcia Denegri, Maria Emília
Leiva, Laura Cristina Ana
Silva Junior, José Antonio
Pavan Zuliani, Juliana
Zamuner, Stella Regina
author_sort Cabral David, Amanda
title Photobiomodulation therapy on local effects induced by juvenile and adult venoms of Bothrops alternatus
title_short Photobiomodulation therapy on local effects induced by juvenile and adult venoms of Bothrops alternatus
title_full Photobiomodulation therapy on local effects induced by juvenile and adult venoms of Bothrops alternatus
title_fullStr Photobiomodulation therapy on local effects induced by juvenile and adult venoms of Bothrops alternatus
title_full_unstemmed Photobiomodulation therapy on local effects induced by juvenile and adult venoms of Bothrops alternatus
title_sort photobiomodulation therapy on local effects induced by juvenile and adult venoms of bothrops alternatus
publisher Elsevier
publishDate 2025
url http://repositorio.unne.edu.ar/handle/123456789/59353
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