Colchicine, trifluralin, and oryzalin promoted development of somatic embryos in Ilex paraguariensis (Aquifoliaceae)

A protocol for somatic embryogenesis and plant regeneration of Ilex paraguariensis St. Hil. from embryos cultures was developed. Heart stage zygotic embryos were removed from seeds of immature, light green fruit and treated with antimicrotubule agents (0.1; 0.2, and 0.5% colchicine for 24 and 48 h;...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Rey, Hebe Yolanda, Sansberro, Pedro Alfonso, Collavino, Mónica Mariana, Daviña, Julio Rubén, González, Ana María, Mroginski, Luis Amado
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer Nature 2025
Materias:
Antimicrotubule agents
Embryo culture
Plant regeneration
Ploidy
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/59341
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-59341
record_format dspace
spelling I48-R184-123456789-593412025-12-15T12:00:20Z Colchicine, trifluralin, and oryzalin promoted development of somatic embryos in Ilex paraguariensis (Aquifoliaceae) Rey, Hebe Yolanda Sansberro, Pedro Alfonso Collavino, Mónica Mariana Daviña, Julio Rubén González, Ana María Mroginski, Luis Amado Antimicrotubule agents Embryo culture Plant regeneration Ploidy A protocol for somatic embryogenesis and plant regeneration of Ilex paraguariensis St. Hil. from embryos cultures was developed. Heart stage zygotic embryos were removed from seeds of immature, light green fruit and treated with antimicrotubule agents (0.1; 0.2, and 0.5% colchicine for 24 and 48 h; 1; 10, and 20 μM of either trifluralin, ααα- trifluoro- 2,6-dinitro-N,N- dipropyl-p-toluidine, or oryzalin, 3,5-dinitro-N4, N-dipropylsulphate during 48 h). The embryos were cultured aseptically on quarter-strength Murashige and Skoog medium containing 3% sucrose, 0.65% agar (1/4MS), and 0.46 μM zeatin. Cultures were incubated in darkness at 27 ± 2 ◦C. All the treatments provoked a diminution of the number of germinated embryos and in some of the treated embryos somatic embryogenesis was induced. Somatic embryo maturation and conversion into whole plants could be achieved by culturing the embryos on 1/4MS lacking hormones and incubated at 27 ± 2 ◦C, 14 h photoperiod (116 μmol m−2 s −1). Most of the plants regenerated from somatic embryos appeared morphologically normal and grew under greenhouse conditions. Only 2 plants out of 152 studied contained the tetraploid number of the chromosomes (2n = 4x = 80), meanwhile the rest of the plants had the normal diploid number of chromosomes (2n = 2x = 40). Somatic embryos with abnormal morphology were also observed. 2025-12-15T11:29:23Z 2025-12-15T11:29:23Z 2002 Artículo Rey, Hebe Yolanda, et al., 2002. Colchicine, trifluralin, and oryzalin promoted development of somatic embryos in Ilex paraguariensis (Aquifoliaceae). Euphytica. Alemania: Springer Nature, vol. 123, p. 49-56. E-ISSN 1573-5060. 0014-2336 http://repositorio.unne.edu.ar/handle/123456789/59341 eng https://link.springer.com/article/10.1023/A:1014489207888 closedAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 49-56 application/pdf Springer Nature Euphytica, 2002, vol. 123, p. 49-56.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Antimicrotubule agents
Embryo culture
Plant regeneration
Ploidy
spellingShingle Antimicrotubule agents
Embryo culture
Plant regeneration
Ploidy
Rey, Hebe Yolanda
Sansberro, Pedro Alfonso
Collavino, Mónica Mariana
Daviña, Julio Rubén
González, Ana María
Mroginski, Luis Amado
Colchicine, trifluralin, and oryzalin promoted development of somatic embryos in Ilex paraguariensis (Aquifoliaceae)
topic_facet Antimicrotubule agents
Embryo culture
Plant regeneration
Ploidy
description A protocol for somatic embryogenesis and plant regeneration of Ilex paraguariensis St. Hil. from embryos cultures was developed. Heart stage zygotic embryos were removed from seeds of immature, light green fruit and treated with antimicrotubule agents (0.1; 0.2, and 0.5% colchicine for 24 and 48 h; 1; 10, and 20 μM of either trifluralin, ααα- trifluoro- 2,6-dinitro-N,N- dipropyl-p-toluidine, or oryzalin, 3,5-dinitro-N4, N-dipropylsulphate during 48 h). The embryos were cultured aseptically on quarter-strength Murashige and Skoog medium containing 3% sucrose, 0.65% agar (1/4MS), and 0.46 μM zeatin. Cultures were incubated in darkness at 27 ± 2 ◦C. All the treatments provoked a diminution of the number of germinated embryos and in some of the treated embryos somatic embryogenesis was induced. Somatic embryo maturation and conversion into whole plants could be achieved by culturing the embryos on 1/4MS lacking hormones and incubated at 27 ± 2 ◦C, 14 h photoperiod (116 μmol m−2 s −1). Most of the plants regenerated from somatic embryos appeared morphologically normal and grew under greenhouse conditions. Only 2 plants out of 152 studied contained the tetraploid number of the chromosomes (2n = 4x = 80), meanwhile the rest of the plants had the normal diploid number of chromosomes (2n = 2x = 40). Somatic embryos with abnormal morphology were also observed.
format Artículo
author Rey, Hebe Yolanda
Sansberro, Pedro Alfonso
Collavino, Mónica Mariana
Daviña, Julio Rubén
González, Ana María
Mroginski, Luis Amado
author_facet Rey, Hebe Yolanda
Sansberro, Pedro Alfonso
Collavino, Mónica Mariana
Daviña, Julio Rubén
González, Ana María
Mroginski, Luis Amado
author_sort Rey, Hebe Yolanda
title Colchicine, trifluralin, and oryzalin promoted development of somatic embryos in Ilex paraguariensis (Aquifoliaceae)
title_short Colchicine, trifluralin, and oryzalin promoted development of somatic embryos in Ilex paraguariensis (Aquifoliaceae)
title_full Colchicine, trifluralin, and oryzalin promoted development of somatic embryos in Ilex paraguariensis (Aquifoliaceae)
title_fullStr Colchicine, trifluralin, and oryzalin promoted development of somatic embryos in Ilex paraguariensis (Aquifoliaceae)
title_full_unstemmed Colchicine, trifluralin, and oryzalin promoted development of somatic embryos in Ilex paraguariensis (Aquifoliaceae)
title_sort colchicine, trifluralin, and oryzalin promoted development of somatic embryos in ilex paraguariensis (aquifoliaceae)
publisher Springer Nature
publishDate 2025
url http://repositorio.unne.edu.ar/handle/123456789/59341
work_keys_str_mv AT reyhebeyolanda colchicinetrifluralinandoryzalinpromoteddevelopmentofsomaticembryosinilexparaguariensisaquifoliaceae
AT sansberropedroalfonso colchicinetrifluralinandoryzalinpromoteddevelopmentofsomaticembryosinilexparaguariensisaquifoliaceae
AT collavinomonicamariana colchicinetrifluralinandoryzalinpromoteddevelopmentofsomaticembryosinilexparaguariensisaquifoliaceae
AT davinajulioruben colchicinetrifluralinandoryzalinpromoteddevelopmentofsomaticembryosinilexparaguariensisaquifoliaceae
AT gonzalezanamaria colchicinetrifluralinandoryzalinpromoteddevelopmentofsomaticembryosinilexparaguariensisaquifoliaceae
AT mroginskiluisamado colchicinetrifluralinandoryzalinpromoteddevelopmentofsomaticembryosinilexparaguariensisaquifoliaceae
_version_ 1857347656528953344

Ejemplares similares