GG13 : a candidate gene related to seed development and viability from apomictic Paspalum notatum
One way to transfer apomixis (asexual reproduction through seeds) to major crops could be through genetic transformation, but for this a set of transgenes that triggers each of the involved processes should be generated. Although most angiosperms require an endospermic balance number (EBN) for norma...
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| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer Nature
2025
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| Materias: | |
| Acceso en línea: | http://repositorio.unne.edu.ar/handle/123456789/59340 |
| Aporte de: |
| Sumario: | One way to transfer apomixis (asexual
reproduction through seeds) to major crops could be
through genetic transformation, but for this a set of
transgenes that triggers each of the involved processes
should be generated. Although most angiosperms
require an endospermic balance number (EBN) for
normal endosperm development, tetraploid Paspalum
notatum is EBN- insensitive. Here, a candidate gene
(GG13) of Paspalum notatum associated with endo-
sperm development in insensitive EBN crosses was
analyzed by gene silencing in Arabidopsis thaliana.
When comparing the silenced and control conditions,
silenced condition evidenced less relative expression
in RT-qPCR experiments, more abortions (6 vs. 1), fewer seeds (16 vs. 34), a more elongated shape (shape
index 1.83 vs. 1.57), and lower germination (4 vs.
36 %) in phenotypic analyses. The above results were
consistent with bioinformatically predicted functions
for GG13 gene. Therefore, the importance of the
GG13 gene in endosperm development in EBN-
insensitive seeds was demonstrated, offering the
possibility of incorporating apomixis into major crops. |
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