GG13 : a candidate gene related to seed development and viability from apomictic Paspalum notatum
One way to transfer apomixis (asexual reproduction through seeds) to major crops could be through genetic transformation, but for this a set of transgenes that triggers each of the involved processes should be generated. Although most angiosperms require an endospermic balance number (EBN) for norma...
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer Nature
2025
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| Acceso en línea: | http://repositorio.unne.edu.ar/handle/123456789/59340 |
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I48-R184-123456789-593402025-12-15T12:00:13Z GG13 : a candidate gene related to seed development and viability from apomictic Paspalum notatum Pozzi, F. I. Acuña, Carlos Alberto Quarin, Camilo Luis Felitti, Silvina Andrea Apomixis Endosperm Endosperm balance number (EBN) HpRNA PTGS Seed development One way to transfer apomixis (asexual reproduction through seeds) to major crops could be through genetic transformation, but for this a set of transgenes that triggers each of the involved processes should be generated. Although most angiosperms require an endospermic balance number (EBN) for normal endosperm development, tetraploid Paspalum notatum is EBN- insensitive. Here, a candidate gene (GG13) of Paspalum notatum associated with endo- sperm development in insensitive EBN crosses was analyzed by gene silencing in Arabidopsis thaliana. When comparing the silenced and control conditions, silenced condition evidenced less relative expression in RT-qPCR experiments, more abortions (6 vs. 1), fewer seeds (16 vs. 34), a more elongated shape (shape index 1.83 vs. 1.57), and lower germination (4 vs. 36 %) in phenotypic analyses. The above results were consistent with bioinformatically predicted functions for GG13 gene. Therefore, the importance of the GG13 gene in endosperm development in EBN- insensitive seeds was demonstrated, offering the possibility of incorporating apomixis into major crops. 2025-12-15T11:29:22Z 2025-12-15T11:29:22Z 2021 Artículo Pozzi, F. I., et al., 2021. GG13 : a candidate gene related to seed development and viability from apomictic Paspalum notatum. Euphytica. Alemania: Springer Nature, vol. 217, no. 148, p. 1-10. E-ISSN 1573-5060. 0014-2336 http://repositorio.unne.edu.ar/handle/123456789/59340 eng https://link.springer.com/article/10.1007/s10681-021-02881-1 closedAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 1-10 application/pdf Springer Nature Euphytica, 2021, vol. 217, no. 148, p. 1-10. |
| institution |
Universidad Nacional del Nordeste |
| institution_str |
I-48 |
| repository_str |
R-184 |
| collection |
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) |
| language |
Inglés |
| topic |
Apomixis Endosperm Endosperm balance number (EBN) HpRNA PTGS Seed development |
| spellingShingle |
Apomixis Endosperm Endosperm balance number (EBN) HpRNA PTGS Seed development Pozzi, F. I. Acuña, Carlos Alberto Quarin, Camilo Luis Felitti, Silvina Andrea GG13 : a candidate gene related to seed development and viability from apomictic Paspalum notatum |
| topic_facet |
Apomixis Endosperm Endosperm balance number (EBN) HpRNA PTGS Seed development |
| description |
One way to transfer apomixis (asexual
reproduction through seeds) to major crops could be
through genetic transformation, but for this a set of
transgenes that triggers each of the involved processes
should be generated. Although most angiosperms
require an endospermic balance number (EBN) for
normal endosperm development, tetraploid Paspalum
notatum is EBN- insensitive. Here, a candidate gene
(GG13) of Paspalum notatum associated with endo-
sperm development in insensitive EBN crosses was
analyzed by gene silencing in Arabidopsis thaliana.
When comparing the silenced and control conditions,
silenced condition evidenced less relative expression
in RT-qPCR experiments, more abortions (6 vs. 1), fewer seeds (16 vs. 34), a more elongated shape (shape
index 1.83 vs. 1.57), and lower germination (4 vs.
36 %) in phenotypic analyses. The above results were
consistent with bioinformatically predicted functions
for GG13 gene. Therefore, the importance of the
GG13 gene in endosperm development in EBN-
insensitive seeds was demonstrated, offering the
possibility of incorporating apomixis into major crops. |
| format |
Artículo |
| author |
Pozzi, F. I. Acuña, Carlos Alberto Quarin, Camilo Luis Felitti, Silvina Andrea |
| author_facet |
Pozzi, F. I. Acuña, Carlos Alberto Quarin, Camilo Luis Felitti, Silvina Andrea |
| author_sort |
Pozzi, F. I. |
| title |
GG13 : a candidate gene related to seed development and viability from apomictic Paspalum notatum |
| title_short |
GG13 : a candidate gene related to seed development and viability from apomictic Paspalum notatum |
| title_full |
GG13 : a candidate gene related to seed development and viability from apomictic Paspalum notatum |
| title_fullStr |
GG13 : a candidate gene related to seed development and viability from apomictic Paspalum notatum |
| title_full_unstemmed |
GG13 : a candidate gene related to seed development and viability from apomictic Paspalum notatum |
| title_sort |
gg13 : a candidate gene related to seed development and viability from apomictic paspalum notatum |
| publisher |
Springer Nature |
| publishDate |
2025 |
| url |
http://repositorio.unne.edu.ar/handle/123456789/59340 |
| work_keys_str_mv |
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