Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography

A new method to determine mixtures of two sweeteners, aspartame and acesulfame-K, in commercial sweeteners is proposed. A classical 52 full factorial design for standards was used for calibration in the concentration matrix. Salicylic acid was used as internal standard in order to evaluate the adjus...

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Autores principales: Cantarelli, Miguel Ángel, Pellerano, Roberto Gerardo, Marchevsky, Eduardo Jorge, Camiña, José Manuel
Formato: Artículo
Lenguaje:Inglés
Publicado: Elsevier 2025
Materias:
Sweeteners
Aspartame
Acesulfame-K
PLS-2 method
Multivariate calibration
HPLC
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/59149
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-59149
record_format dspace
spelling I48-R184-123456789-591492025-12-05T11:21:46Z Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography Cantarelli, Miguel Ángel Pellerano, Roberto Gerardo Marchevsky, Eduardo Jorge Camiña, José Manuel Sweeteners Aspartame Acesulfame-K PLS-2 method Multivariate calibration HPLC A new method to determine mixtures of two sweeteners, aspartame and acesulfame-K, in commercial sweeteners is proposed. A classical 52 full factorial design for standards was used for calibration in the concentration matrix. Salicylic acid was used as internal standard in order to evaluate the adjustment of the real samples in the PLS-2 model. This model was obtained from UV spectral data, validated by internal cross-validation and was used to find the concentration of analytes in the commercial samples. The PLS-2 method was validated externally by high performance liquid chromatography (HPLC), finding, in all cases, a relative error of less than 10% between the PLS-2 and the HPLC methods. The mean value of recovery degree in real samples was 99.2% with standard deviation of 3.2%. The proposed procedure was applied successfully to the determination of mixtures of aspartame and acesulfame-K in artificial sweeteners. 2025-12-05T11:11:42Z 2025-12-05T11:11:42Z 2009-08-01 Artículo Cantarelli, Miguel Ángel, et al., 2009. Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography. Food Chemistry. Ámsterdam: Países Bajos, vol. 115, p. 1128-1132. E-ISSN 2772-753X. DOI https://doi.org/10.1016/j.foodchem.2008.12.101 http://repositorio.unne.edu.ar/handle/123456789/59149 en https://doi.org/10.1016/j.foodchem.2008.12.101 restrictedAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 1128-1132 application/pdf Elsevier Food Chemistry, 2009, vol. 115, no. 3, p. 1128-1132.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Sweeteners
Aspartame
Acesulfame-K
PLS-2 method
Multivariate calibration
HPLC
spellingShingle Sweeteners
Aspartame
Acesulfame-K
PLS-2 method
Multivariate calibration
HPLC
Cantarelli, Miguel Ángel
Pellerano, Roberto Gerardo
Marchevsky, Eduardo Jorge
Camiña, José Manuel
Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography
topic_facet Sweeteners
Aspartame
Acesulfame-K
PLS-2 method
Multivariate calibration
HPLC
description A new method to determine mixtures of two sweeteners, aspartame and acesulfame-K, in commercial sweeteners is proposed. A classical 52 full factorial design for standards was used for calibration in the concentration matrix. Salicylic acid was used as internal standard in order to evaluate the adjustment of the real samples in the PLS-2 model. This model was obtained from UV spectral data, validated by internal cross-validation and was used to find the concentration of analytes in the commercial samples. The PLS-2 method was validated externally by high performance liquid chromatography (HPLC), finding, in all cases, a relative error of less than 10% between the PLS-2 and the HPLC methods. The mean value of recovery degree in real samples was 99.2% with standard deviation of 3.2%. The proposed procedure was applied successfully to the determination of mixtures of aspartame and acesulfame-K in artificial sweeteners.
format Artículo
author Cantarelli, Miguel Ángel
Pellerano, Roberto Gerardo
Marchevsky, Eduardo Jorge
Camiña, José Manuel
author_facet Cantarelli, Miguel Ángel
Pellerano, Roberto Gerardo
Marchevsky, Eduardo Jorge
Camiña, José Manuel
author_sort Cantarelli, Miguel Ángel
title Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography
title_short Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography
title_full Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography
title_fullStr Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography
title_full_unstemmed Simultaneous determination of aspartame and acesulfame-K by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography
title_sort simultaneous determination of aspartame and acesulfame-k by molecular absorption spectrophotometry using multivariate calibration and validation by high performance liquid chromatography
publisher Elsevier
publishDate 2025
url http://repositorio.unne.edu.ar/handle/123456789/59149
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AT marchevskyeduardojorge simultaneousdeterminationofaspartameandacesulfamekbymolecularabsorptionspectrophotometryusingmultivariatecalibrationandvalidationbyhighperformanceliquidchromatography
AT caminajosemanuel simultaneousdeterminationofaspartameandacesulfamekbymolecularabsorptionspectrophotometryusingmultivariatecalibrationandvalidationbyhighperformanceliquidchromatography
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