In vitro cultured primary roots derived from stem segments of Cassava (Manihot esculenta) can behave like storage organs

Background and Aims: Cassava ( Manihot esculenta ) has three adventitious root types: primary and secondary fibrous roots, and storage roots. Different adventitious root types can also regenerate from in vitro cultured segments. The aim of this study was to investigate aspects of in vitro production...

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Autores principales: Medina, Ricardo Daniel, Faloci, Mirta Mercedes, González, Ana María, Mroginski, Luis Alberto
Formato: Artículo
Lenguaje:Inglés
Publicado: Oxford University Press 2025
Materias:
Auxins
cassava
cytokinins
in vitro root regeneration
Manihot esculenta
morphogenesis
storage organs
tuberization
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/58288
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-58288
record_format dspace
spelling I48-R184-123456789-582882025-08-28T13:59:59Z In vitro cultured primary roots derived from stem segments of Cassava (Manihot esculenta) can behave like storage organs Medina, Ricardo Daniel Faloci, Mirta Mercedes González, Ana María Mroginski, Luis Alberto Auxins cassava cytokinins in vitro root regeneration Manihot esculenta morphogenesis storage organs tuberization Background and Aims: Cassava ( Manihot esculenta ) has three adventitious root types: primary and secondary fibrous roots, and storage roots. Different adventitious root types can also regenerate from in vitro cultured segments. The aim of this study was to investigate aspects of in vitro production of storage roots. Methods: Morphological and anatomical analyses were performed to identify and differentiate each root type. Twenty-nine clones were assayed to determine the effect of genotype on the capacity to form storage roots in vitro . The effects of cytokinins and auxins on the formation of storage roots in vitro were also examined. Key Results: Primary roots formed in vitro and in vivo had similar tissue kinds; however, storage roots formed in vitro exhibited physiological specialization for storing starch. The only consistent diagnostic feature between secondary fibrous and storage roots was their functional differentiation. Anatomical analysis of the storage roots formed in vitro showed that radial expansion as a consequence of massive proliferation and enlargement of parenchymatous cells occurred in the middle cortex, but not from cambial activity as in roots formed in vivo . Cortical expansion could be related to dilatation growth favoured by hormone treatments. Starch deposition of storage roots formed in vitro was confined to cortical tissue and occurred earlier than in storage roots formed in vivo . Auxin and cytokinin supplementation were absolutely required for in vitro storage root regeneration; these roots were not able to develop secondary growth, but formed a tissue competent for starch storing. MS medium with 5 % sucrose plus 0·54 μ m 1-naphthaleneacetic acid and 0·44 μ m 6-benzylaminopurine was one of the most effective in stimulating the storage root formation. Genotypes differed significantly in their capacity to produce storage roots in vitro . Storage root formation was considerably affected by the segment's primary position and strongly influenced by hormone treatments. Conclusions: The storage root formation system reported here is a first approach to develop a tuberization model, and additional efforts are required to improve it. Although it was not possible to achieve root secondary growth, after this work it will be feasible to advance in some aspects of in vitro cassava tuberization. 2025-08-28T13:36:56Z 2025-08-28T13:36:56Z 2007-03-01 Artículo Medina, Ricardo Daniel, et al., 2007. In vitro cultured primary roots derived from stem segments of Cassava (Manihot esculenta) can behave like storage organs. Annals of Botany. Oxford: Oxford University Press, vol. 99, no. 3, p. 409-423. E-ISSN 1095-8290. DOI https://doi.org/10.1093/aob/mcl272 0305-7364 http://repositorio.unne.edu.ar/handle/123456789/58288 eng https://doi.org/10.1093/aob/mcl272 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 409-423 application/pdf Oxford University Press Annals of Botany, 2007, vol. 99, no. 3, p. 409-423.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Auxins
cassava
cytokinins
in vitro root regeneration
Manihot esculenta
morphogenesis
storage organs
tuberization
spellingShingle Auxins
cassava
cytokinins
in vitro root regeneration
Manihot esculenta
morphogenesis
storage organs
tuberization
Medina, Ricardo Daniel
Faloci, Mirta Mercedes
González, Ana María
Mroginski, Luis Alberto
In vitro cultured primary roots derived from stem segments of Cassava (Manihot esculenta) can behave like storage organs
topic_facet Auxins
cassava
cytokinins
in vitro root regeneration
Manihot esculenta
morphogenesis
storage organs
tuberization
description Background and Aims: Cassava ( Manihot esculenta ) has three adventitious root types: primary and secondary fibrous roots, and storage roots. Different adventitious root types can also regenerate from in vitro cultured segments. The aim of this study was to investigate aspects of in vitro production of storage roots. Methods: Morphological and anatomical analyses were performed to identify and differentiate each root type. Twenty-nine clones were assayed to determine the effect of genotype on the capacity to form storage roots in vitro . The effects of cytokinins and auxins on the formation of storage roots in vitro were also examined. Key Results: Primary roots formed in vitro and in vivo had similar tissue kinds; however, storage roots formed in vitro exhibited physiological specialization for storing starch. The only consistent diagnostic feature between secondary fibrous and storage roots was their functional differentiation. Anatomical analysis of the storage roots formed in vitro showed that radial expansion as a consequence of massive proliferation and enlargement of parenchymatous cells occurred in the middle cortex, but not from cambial activity as in roots formed in vivo . Cortical expansion could be related to dilatation growth favoured by hormone treatments. Starch deposition of storage roots formed in vitro was confined to cortical tissue and occurred earlier than in storage roots formed in vivo . Auxin and cytokinin supplementation were absolutely required for in vitro storage root regeneration; these roots were not able to develop secondary growth, but formed a tissue competent for starch storing. MS medium with 5 % sucrose plus 0·54 μ m 1-naphthaleneacetic acid and 0·44 μ m 6-benzylaminopurine was one of the most effective in stimulating the storage root formation. Genotypes differed significantly in their capacity to produce storage roots in vitro . Storage root formation was considerably affected by the segment's primary position and strongly influenced by hormone treatments. Conclusions: The storage root formation system reported here is a first approach to develop a tuberization model, and additional efforts are required to improve it. Although it was not possible to achieve root secondary growth, after this work it will be feasible to advance in some aspects of in vitro cassava tuberization.
format Artículo
author Medina, Ricardo Daniel
Faloci, Mirta Mercedes
González, Ana María
Mroginski, Luis Alberto
author_facet Medina, Ricardo Daniel
Faloci, Mirta Mercedes
González, Ana María
Mroginski, Luis Alberto
author_sort Medina, Ricardo Daniel
title In vitro cultured primary roots derived from stem segments of Cassava (Manihot esculenta) can behave like storage organs
title_short In vitro cultured primary roots derived from stem segments of Cassava (Manihot esculenta) can behave like storage organs
title_full In vitro cultured primary roots derived from stem segments of Cassava (Manihot esculenta) can behave like storage organs
title_fullStr In vitro cultured primary roots derived from stem segments of Cassava (Manihot esculenta) can behave like storage organs
title_full_unstemmed In vitro cultured primary roots derived from stem segments of Cassava (Manihot esculenta) can behave like storage organs
title_sort in vitro cultured primary roots derived from stem segments of cassava (manihot esculenta) can behave like storage organs
publisher Oxford University Press
publishDate 2025
url http://repositorio.unne.edu.ar/handle/123456789/58288
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AT gonzalezanamaria invitroculturedprimaryrootsderivedfromstemsegmentsofcassavamanihotesculentacanbehavelikestorageorgans
AT mroginskiluisalberto invitroculturedprimaryrootsderivedfromstemsegmentsofcassavamanihotesculentacanbehavelikestorageorgans
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