Effect of pollination timing on the rate of apomictic reproduction revealed by RAPD Markers in Paspalum notatum

Progeny tests employing molecular markers allow the identification of individuals originated by sexual means among the offspring of a facultative apomict. The objective of this work was to evaluate the effect of the pollination timing on the proportion of sexually formed individuals in progenies of...

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Autores principales: Espinoza, Francisco, Pessino, Silvina Claudia, Quarín, Camilo León, Valle, E. M.
Formato: Artículo
Lenguaje:Inglés
Publicado: Oxford University Press 2025
Materias:
Apomixis
Paspalum notatum
pollination timing
RAPD fingerprinting
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/58285
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RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-58285
record_format dspace
spelling I48-R184-123456789-582852025-08-28T13:59:37Z Effect of pollination timing on the rate of apomictic reproduction revealed by RAPD Markers in Paspalum notatum Espinoza, Francisco Pessino, Silvina Claudia Quarín, Camilo León Valle, E. M. Apomixis Paspalum notatum pollination timing RAPD fingerprinting Progeny tests employing molecular markers allow the identification of individuals originated by sexual means among the offspring of a facultative apomict. The objective of this work was to evaluate the effect of the pollination timing on the proportion of sexually formed individuals in progenies of a facultative apomictic Paspalum notatum genotype. Progeny families of approx. 30 plants each were generated at five different pollination times: 1–3 d pre‐anthesis; at anthesis; and 2, 4 and 6 d post‐anthesis. Cytoembryological analyses indicated that approx. 17 % of the ovules carried a meiotic cytologically reduced embryo sac in florets formed simultaneously with those used for crosses. The parental plants and the five F1 families were analysed using RAPD molecular markers. Ninety‐five oligonucleotides were assayed on the progenitors in order to search for male‐specific bands. Eight primers presenting clear polymorphic bands were selected for use in the progeny tests. The proportion of sexually produced progeny reached 3·4 % before anthesis and 20 % at anthesis, while pollination after anthesis generated only maternal plants. A second progeny of 97 plants obtained from pollination at anthesis produced 16 off‐type plants (16·5 %), of which only one was a BIII hybrid (2n + n). Our results indicate that pollination at anthesis allows the greatest potential for sexuality to be expressed in this facultative apomictic genotype. When pollination is delayed as soon as 2 d after anthesis, only the aposporous sacs develop endosperm through pseudogamy to set seed. 2025-08-28T13:36:55Z 2025-08-28T13:36:55Z 2002-02-01 Artículo Espinoza, Francisco, et al., 2002. Effect of pollination timing on the rate of apomictic reproduction revealed by RAPD Markers in Paspalum notatum. Annals of Botany. Oxford: Oxford University Press, vol. 89, no. 2, p. 165-170. E-ISSN 1095-8290. DOI https://doi.org/10.1093/aob/mcf024 0305-7364 http://repositorio.unne.edu.ar/handle/123456789/58285 eng https://doi.org/10.1093/aob/mcf024 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 165-170 application/pdf Oxford University Press Annals of Botany, 2002, vol. 89, no. 2, p. 165-170.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Apomixis
Paspalum notatum
pollination timing
RAPD fingerprinting
spellingShingle Apomixis
Paspalum notatum
pollination timing
RAPD fingerprinting
Espinoza, Francisco
Pessino, Silvina Claudia
Quarín, Camilo León
Valle, E. M.
Effect of pollination timing on the rate of apomictic reproduction revealed by RAPD Markers in Paspalum notatum
topic_facet Apomixis
Paspalum notatum
pollination timing
RAPD fingerprinting
description Progeny tests employing molecular markers allow the identification of individuals originated by sexual means among the offspring of a facultative apomict. The objective of this work was to evaluate the effect of the pollination timing on the proportion of sexually formed individuals in progenies of a facultative apomictic Paspalum notatum genotype. Progeny families of approx. 30 plants each were generated at five different pollination times: 1–3 d pre‐anthesis; at anthesis; and 2, 4 and 6 d post‐anthesis. Cytoembryological analyses indicated that approx. 17 % of the ovules carried a meiotic cytologically reduced embryo sac in florets formed simultaneously with those used for crosses. The parental plants and the five F1 families were analysed using RAPD molecular markers. Ninety‐five oligonucleotides were assayed on the progenitors in order to search for male‐specific bands. Eight primers presenting clear polymorphic bands were selected for use in the progeny tests. The proportion of sexually produced progeny reached 3·4 % before anthesis and 20 % at anthesis, while pollination after anthesis generated only maternal plants. A second progeny of 97 plants obtained from pollination at anthesis produced 16 off‐type plants (16·5 %), of which only one was a BIII hybrid (2n + n). Our results indicate that pollination at anthesis allows the greatest potential for sexuality to be expressed in this facultative apomictic genotype. When pollination is delayed as soon as 2 d after anthesis, only the aposporous sacs develop endosperm through pseudogamy to set seed.
format Artículo
author Espinoza, Francisco
Pessino, Silvina Claudia
Quarín, Camilo León
Valle, E. M.
author_facet Espinoza, Francisco
Pessino, Silvina Claudia
Quarín, Camilo León
Valle, E. M.
author_sort Espinoza, Francisco
title Effect of pollination timing on the rate of apomictic reproduction revealed by RAPD Markers in Paspalum notatum
title_short Effect of pollination timing on the rate of apomictic reproduction revealed by RAPD Markers in Paspalum notatum
title_full Effect of pollination timing on the rate of apomictic reproduction revealed by RAPD Markers in Paspalum notatum
title_fullStr Effect of pollination timing on the rate of apomictic reproduction revealed by RAPD Markers in Paspalum notatum
title_full_unstemmed Effect of pollination timing on the rate of apomictic reproduction revealed by RAPD Markers in Paspalum notatum
title_sort effect of pollination timing on the rate of apomictic reproduction revealed by rapd markers in paspalum notatum
publisher Oxford University Press
publishDate 2025
url http://repositorio.unne.edu.ar/handle/123456789/58285
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