Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina

The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in t...

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Autores principales:	Fusco, Luciano S., Neto, Emídio Beraldo, Francisco, Aleff F., Alfonso, Jorge, Soares, Andreimar, Pimenta, Daniel C., Leiva, Laura Cristina Ana
Formato:	Artículo
Lenguaje:	Español
Publicado:	Elsevier Ltd. 2025
Materias:	Crotalus durissus terrificus Northeastern Argentina
Acceso en línea:	http://repositorio.unne.edu.ar/handle/123456789/58047
Aporte de:	RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste

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spelling	I48-R184-123456789-580472025-10-22T11:42:55Z Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina Fusco, Luciano S. Neto, Emídio Beraldo Francisco, Aleff F. Alfonso, Jorge Soares, Andreimar Pimenta, Daniel C. Leiva, Laura Cristina Ana Crotalus durissus terrificus Northeastern Argentina The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom’s composition phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metaloprotease, phospholipase B and 50 -nucleotidase) and proteins (Bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d.terrificus venom from Argentina. This proved to be crotamine positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy. 2025-08-20T10:38:56Z 2025-08-20T10:38:56Z 2020-09-09 Artículo Fusco, Luciano S., et. al, 2020. Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina. Toxicon: X: Países BajosElsevier Ltd., 100047, ISSN 041-0101. 0041-0101 http://repositorio.unne.edu.ar/handle/123456789/58047 spa https://doi.org/10.1016/j.toxcx.2020.100047 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf 100047 application/pdf Elsevier Ltd. Toxicon: X, 2020, vol. 7, 100047
institution	Universidad Nacional del Nordeste
institution_str	I-48
repository_str	R-184
collection	RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language	Español
topic	Crotalus durissus terrificus Northeastern Argentina
spellingShingle	Crotalus durissus terrificus Northeastern Argentina Fusco, Luciano S. Neto, Emídio Beraldo Francisco, Aleff F. Alfonso, Jorge Soares, Andreimar Pimenta, Daniel C. Leiva, Laura Cristina Ana Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
topic_facet	Crotalus durissus terrificus Northeastern Argentina
description	The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom’s composition phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metaloprotease, phospholipase B and 50 -nucleotidase) and proteins (Bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d.terrificus venom from Argentina. This proved to be crotamine positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy.
format	Artículo
author	Fusco, Luciano S. Neto, Emídio Beraldo Francisco, Aleff F. Alfonso, Jorge Soares, Andreimar Pimenta, Daniel C. Leiva, Laura Cristina Ana
author_facet	Fusco, Luciano S. Neto, Emídio Beraldo Francisco, Aleff F. Alfonso, Jorge Soares, Andreimar Pimenta, Daniel C. Leiva, Laura Cristina Ana
author_sort	Fusco, Luciano S.
title	Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_short	Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_full	Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_fullStr	Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_full_unstemmed	Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
title_sort	fast venomic analysis of crotalus durissus terrificus from northeastern argentina
publisher	Elsevier Ltd.
publishDate	2025
url	http://repositorio.unne.edu.ar/handle/123456789/58047
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Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina

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