Hepatic encephalopathy in swine experimentally poisoned with Senna occidentalis seeds: Effects on astrocytes

Senna occidentalis may be accidently ingested by humans and animals. In this study, the percentages of S. occidentalis seeds necessary for experimental reproduction of hepatic encephalopathy were determined in a pig model and the biochemical and microscopic pathology is described in detail, with emp...

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Autores principales: Chileski, Gabriela Soledad, García, Enrique Nicolas, Lértora, Javier Walter, Mussart, Norma, Hernandez, David Roque, Cholich, Luciana Andrea
Formato: Artículo
Lenguaje:Español
Publicado: Elsevier Ltd. 2025
Materias:
Brain, hyperammonaemia
Glia
GFAP
Liver
Senna occidentalis
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/58046
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-58046
record_format dspace
spelling I48-R184-123456789-580462025-08-20T11:03:05Z Hepatic encephalopathy in swine experimentally poisoned with Senna occidentalis seeds: Effects on astrocytes Chileski, Gabriela Soledad García, Enrique Nicolas Lértora, Javier Walter Mussart, Norma Hernandez, David Roque Cholich, Luciana Andrea Brain, hyperammonaemia Glia GFAP Liver Senna occidentalis Senna occidentalis may be accidently ingested by humans and animals. In this study, the percentages of S. occidentalis seeds necessary for experimental reproduction of hepatic encephalopathy were determined in a pig model and the biochemical and microscopic pathology is described in detail, with emphasis on the astrocytes. The experimental groups (G1, G2 and G3) were fed rations containing 5%, 7.5% and 10% of S. occidentalis seeds for 7–11 days. Pigs from the three experimental groups showed incoordination, ataxia, disorientation, head pressing, anorexia, recumbency and depression. In addition, the enzymes aspartate aminotransferase, alkaline phosphatase and creatine phosphokinase increased in all treated animals, which also showed higher serum total bilirubin and ammonia levels than in the control group (C). Microscopically, all experimental animals revealed acute hepatocellular swelling, multifocal coagulative necrosis in the pancreas, necrosis in the cardiac muscle, severe spongiosis in brain white and grey matter, and Alzheimer type II astrocytes in grey matter of the cerebral cortex. These cells were glial fibrillary acidic protein (GFAP) negative in G3. In white matter, a decrease in the positive area occupied by GFAP-immunolabelling and in the number of astrocytes per immunoreactive area was observed in G3 animals (5.35 ± 1.14% and 410 ± 45 cells/mm2 , respectively) compared to the C animals (13.93 ± 1.59% and 581 ± 36 cells/mm2 , respectively). This loss of GFAP was accompanied by alterations in astrocyte morphology, such as shrinkage of the cell body and retraction of the extending processes. This pig model of ammonia-mediated astrocyte damage could be used to study not only poisoning by S. occidentalis, but also other medical conditions resulting in hepatoencephalopathy. 2025-08-20T10:38:55Z 2025-08-20T10:38:55Z 2021-10-21 Artículo Chileski, Gabriela Soledad, et. al, 2021. Hepatic encephalopathy in swine experimentally poisoned with Senna occidentalis seeds: Effects on astrocytes. Toxicon: Países BajosElsevier Ltd, p. 86-91, ISSN 0041-0101. 0041-0101 http://repositorio.unne.edu.ar/handle/123456789/58046 spa https://doi.org/10.1016/j.toxicon.2021.08.018 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 86-91 application/pdf Elsevier Ltd. Toxicon, 2021, vol. 201, p. 86-91.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Español
topic Brain, hyperammonaemia
Glia
GFAP
Liver
Senna occidentalis
spellingShingle Brain, hyperammonaemia
Glia
GFAP
Liver
Senna occidentalis
Chileski, Gabriela Soledad
García, Enrique Nicolas
Lértora, Javier Walter
Mussart, Norma
Hernandez, David Roque
Cholich, Luciana Andrea
Hepatic encephalopathy in swine experimentally poisoned with Senna occidentalis seeds: Effects on astrocytes
topic_facet Brain, hyperammonaemia
Glia
GFAP
Liver
Senna occidentalis
description Senna occidentalis may be accidently ingested by humans and animals. In this study, the percentages of S. occidentalis seeds necessary for experimental reproduction of hepatic encephalopathy were determined in a pig model and the biochemical and microscopic pathology is described in detail, with emphasis on the astrocytes. The experimental groups (G1, G2 and G3) were fed rations containing 5%, 7.5% and 10% of S. occidentalis seeds for 7–11 days. Pigs from the three experimental groups showed incoordination, ataxia, disorientation, head pressing, anorexia, recumbency and depression. In addition, the enzymes aspartate aminotransferase, alkaline phosphatase and creatine phosphokinase increased in all treated animals, which also showed higher serum total bilirubin and ammonia levels than in the control group (C). Microscopically, all experimental animals revealed acute hepatocellular swelling, multifocal coagulative necrosis in the pancreas, necrosis in the cardiac muscle, severe spongiosis in brain white and grey matter, and Alzheimer type II astrocytes in grey matter of the cerebral cortex. These cells were glial fibrillary acidic protein (GFAP) negative in G3. In white matter, a decrease in the positive area occupied by GFAP-immunolabelling and in the number of astrocytes per immunoreactive area was observed in G3 animals (5.35 ± 1.14% and 410 ± 45 cells/mm2 , respectively) compared to the C animals (13.93 ± 1.59% and 581 ± 36 cells/mm2 , respectively). This loss of GFAP was accompanied by alterations in astrocyte morphology, such as shrinkage of the cell body and retraction of the extending processes. This pig model of ammonia-mediated astrocyte damage could be used to study not only poisoning by S. occidentalis, but also other medical conditions resulting in hepatoencephalopathy.
format Artículo
author Chileski, Gabriela Soledad
García, Enrique Nicolas
Lértora, Javier Walter
Mussart, Norma
Hernandez, David Roque
Cholich, Luciana Andrea
author_facet Chileski, Gabriela Soledad
García, Enrique Nicolas
Lértora, Javier Walter
Mussart, Norma
Hernandez, David Roque
Cholich, Luciana Andrea
author_sort Chileski, Gabriela Soledad
title Hepatic encephalopathy in swine experimentally poisoned with Senna occidentalis seeds: Effects on astrocytes
title_short Hepatic encephalopathy in swine experimentally poisoned with Senna occidentalis seeds: Effects on astrocytes
title_full Hepatic encephalopathy in swine experimentally poisoned with Senna occidentalis seeds: Effects on astrocytes
title_fullStr Hepatic encephalopathy in swine experimentally poisoned with Senna occidentalis seeds: Effects on astrocytes
title_full_unstemmed Hepatic encephalopathy in swine experimentally poisoned with Senna occidentalis seeds: Effects on astrocytes
title_sort hepatic encephalopathy in swine experimentally poisoned with senna occidentalis seeds: effects on astrocytes
publisher Elsevier Ltd.
publishDate 2025
url http://repositorio.unne.edu.ar/handle/123456789/58046
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AT lertorajavierwalter hepaticencephalopathyinswineexperimentallypoisonedwithsennaoccidentalisseedseffectsonastrocytes
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