Isolation of two basic phospholipases A2 from Bothrops diporus snake venom : Comparative characterization and synergism between Asp49 and Lys49 variants

Bothrops diporus, previously considered a subspecies of the B. neuwiedi complex, is a medically relevant viperid in Northeastern Argentina. The venom of this species causes local tissue damage characterized by myonecrosis, hemorrhage, blistering, and edema. In the present study, two basic phosphol...

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Autores principales: Bustillo, Soledad, Fernández, Julián, Chaves-Araya, Stephanie, Angulo, Yamileth, Leiva, Laura Cristina Ana, Lomonte, Bruno
Formato: Artículo
Lenguaje:Español
Publicado: Elsevier Ltd. 2025
Materias:
Snake venom
Bothrops diporus
Phospholipase A2
Synergism
Myotoxicity
Cytotoxicity
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/58045
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-58045
record_format dspace
spelling I48-R184-123456789-580452025-10-22T11:44:55Z Isolation of two basic phospholipases A2 from Bothrops diporus snake venom : Comparative characterization and synergism between Asp49 and Lys49 variants Bustillo, Soledad Fernández, Julián Chaves-Araya, Stephanie Angulo, Yamileth Leiva, Laura Cristina Ana Lomonte, Bruno Snake venom Bothrops diporus Phospholipase A2 Synergism Myotoxicity Cytotoxicity Bothrops diporus, previously considered a subspecies of the B. neuwiedi complex, is a medically relevant viperid in Northeastern Argentina. The venom of this species causes local tissue damage characterized by myonecrosis, hemorrhage, blistering, and edema. In the present study, two basic phospholipases A2 (PLA2-I and PLA2-II) were isolated from this venom, and their pathological effects upon murine skeletal muscle and myogenic cells in culture were analyzed. Partial amino acid sequencing showed that PLA2-I and PLA2-II are Asp49 and Lys49 PLA2s, respectively. In agreement with this, PLA2-I showed PLA2 activity, whereas PLA2-II did not. Functional assays revealed differences in their myotoxicity, cytotoxicity, and anti-adhesion activity, and in the ability to inhibit cell migration, all of which were greater for the Lys49 variant. Native electrophoresis showed that PLA2-I was less basic than PLA2-II. The two proteins act synergistically to affect the integrity of C2C12 myogenic cells, providing a further example of the concerted action of coexisting snake venom components. PLA2-I and PLA2-II, together with additional basic PLA2s revealed by RP-HPLC, probably play an important role in myonecrosis after envenomation by B. diporus. 2025-08-20T10:38:55Z 2025-08-20T10:38:55Z 2019-10-16 Artículo Bustillo, Soledad, et. al, 2019. Isolation of two basic phospholipases A2 from Bothrops diporus snake venom : Comparative characterization and synergism between Asp49 and Lys49 variants. Toxicon: Países Bajos, Elsevier Ltd., p. 113-121, ISSN 0041-0101. 0041-0101 http://repositorio.unne.edu.ar/handle/123456789/58045 spa https://doi.org/10.1016/j.toxicon.2019.07.004 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 113-121 application/pdf Elsevier Ltd. Toxicon, 2019, vol. 168, p. 113-121.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Español
topic Snake venom
Bothrops diporus
Phospholipase A2
Synergism
Myotoxicity
Cytotoxicity
spellingShingle Snake venom
Bothrops diporus
Phospholipase A2
Synergism
Myotoxicity
Cytotoxicity
Bustillo, Soledad
Fernández, Julián
Chaves-Araya, Stephanie
Angulo, Yamileth
Leiva, Laura Cristina Ana
Lomonte, Bruno
Isolation of two basic phospholipases A2 from Bothrops diporus snake venom : Comparative characterization and synergism between Asp49 and Lys49 variants
topic_facet Snake venom
Bothrops diporus
Phospholipase A2
Synergism
Myotoxicity
Cytotoxicity
description Bothrops diporus, previously considered a subspecies of the B. neuwiedi complex, is a medically relevant viperid in Northeastern Argentina. The venom of this species causes local tissue damage characterized by myonecrosis, hemorrhage, blistering, and edema. In the present study, two basic phospholipases A2 (PLA2-I and PLA2-II) were isolated from this venom, and their pathological effects upon murine skeletal muscle and myogenic cells in culture were analyzed. Partial amino acid sequencing showed that PLA2-I and PLA2-II are Asp49 and Lys49 PLA2s, respectively. In agreement with this, PLA2-I showed PLA2 activity, whereas PLA2-II did not. Functional assays revealed differences in their myotoxicity, cytotoxicity, and anti-adhesion activity, and in the ability to inhibit cell migration, all of which were greater for the Lys49 variant. Native electrophoresis showed that PLA2-I was less basic than PLA2-II. The two proteins act synergistically to affect the integrity of C2C12 myogenic cells, providing a further example of the concerted action of coexisting snake venom components. PLA2-I and PLA2-II, together with additional basic PLA2s revealed by RP-HPLC, probably play an important role in myonecrosis after envenomation by B. diporus.
format Artículo
author Bustillo, Soledad
Fernández, Julián
Chaves-Araya, Stephanie
Angulo, Yamileth
Leiva, Laura Cristina Ana
Lomonte, Bruno
author_facet Bustillo, Soledad
Fernández, Julián
Chaves-Araya, Stephanie
Angulo, Yamileth
Leiva, Laura Cristina Ana
Lomonte, Bruno
author_sort Bustillo, Soledad
title Isolation of two basic phospholipases A2 from Bothrops diporus snake venom : Comparative characterization and synergism between Asp49 and Lys49 variants
title_short Isolation of two basic phospholipases A2 from Bothrops diporus snake venom : Comparative characterization and synergism between Asp49 and Lys49 variants
title_full Isolation of two basic phospholipases A2 from Bothrops diporus snake venom : Comparative characterization and synergism between Asp49 and Lys49 variants
title_fullStr Isolation of two basic phospholipases A2 from Bothrops diporus snake venom : Comparative characterization and synergism between Asp49 and Lys49 variants
title_full_unstemmed Isolation of two basic phospholipases A2 from Bothrops diporus snake venom : Comparative characterization and synergism between Asp49 and Lys49 variants
title_sort isolation of two basic phospholipases a2 from bothrops diporus snake venom : comparative characterization and synergism between asp49 and lys49 variants
publisher Elsevier Ltd.
publishDate 2025
url http://repositorio.unne.edu.ar/handle/123456789/58045
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