A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules

Aposporous apomictic plants form clonal maternal seeds by inducing the emergence of non-reduced (2n) embryo sacs in the ovule nucellus and the development of embryos by parthenogenesis. In previous work, we reported a plant-specific TRIMETHYLGUANOSINE SYNTHASE 1 (TGS1) gene (PN_TGS1-like) showing...

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Autores principales: Colono, Carolina, Ortiz, Juan Pablo Amelio, Permingeat, Hugo Raúl, Souza Canada, Eduardo Daniel, Siena, Lorena Adelina, Spoto, Nicolás, Galdeano, Florencia, Espinoza, Francisco, Leblanc, Olivier, Pessino, Silvina Claudia
Formato: Artículo
Lenguaje:Inglés
Publicado: Universidad de Carolina del Norte 2024
Materias:
Apomixis
Apospory
Methyltransferase
Plant reproduction
TGS1
Acceso en línea:http://repositorio.unne.edu.ar/handle/123456789/56240
Aporte de:
RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) de Universidad Nacional del Nordeste
id I48-R184-123456789-56240
record_format dspace
spelling I48-R184-123456789-562402025-08-29T11:13:51Z A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules Colono, Carolina Ortiz, Juan Pablo Amelio Permingeat, Hugo Raúl Souza Canada, Eduardo Daniel Siena, Lorena Adelina Spoto, Nicolás Galdeano, Florencia Espinoza, Francisco Leblanc, Olivier Pessino, Silvina Claudia Apomixis Apospory Methyltransferase Plant reproduction TGS1 Aposporous apomictic plants form clonal maternal seeds by inducing the emergence of non-reduced (2n) embryo sacs in the ovule nucellus and the development of embryos by parthenogenesis. In previous work, we reported a plant-specific TRIMETHYLGUANOSINE SYNTHASE 1 (TGS1) gene (PN_TGS1-like) showing expression levels positively correlated with sexuality rates in facultative apomictic Paspalum notatum. PN_ TGS1-like displayed contrasting in situ hybridization patterns in apomictic and sexual plant ovules from premeiosis to anthesis. Here we transformed sexual P. notatum with a TGS1-like antisense construction under a constitutive promoter, in order to produce lines with reduced transcript representation. Antisense plants developed prominent trichomes on the adaxial leaf surface, a trait absent from control genotypes. Reproductive development analysis revealed occasional formation of twin ovules. While control individuals typically displayed a single meiotic embryo sac per ovule, antisense lines showed 12.93–15.79% of ovules bearing extra nuclei, which can be assigned to aposporous-like embryo sacs (AES-like) or, alternatively, to gametophytes with a misguided cell fate development. Moreover, around 8.42–9.52% of ovules showed what looked like a combination of meiotic and aposporous-like sacs. Besides, 32.5% of ovules at early developmental stages displayed nucellar cells with prominent nuclei resembling apospory initials (AIs), which surrounded the megaspore mother cell (MMC) or the MMC-derived meiotic products. Two or more concurrent meiosis events were never detected, which suggest a non-reduced nature for the extra nuclei observed in the mature ovules, unless they were generated by proliferation and misguided differentiation of the legitimate meiotic products. The antisense lines produced a similar amount of viable even-sized pollen with respect to control genotypes, and formed an equivalent full seed set (~9% of total seeds) after selfpollination. Flow cytometry analyses of caryopses derived from antisense lines revealed that all full seeds had originated from meiotic embryo sacs (i.e. by sexuality). A reduction of 25.55% in the germination percentage was detected when comparing antisense lines with controls. Our results indicate that PN_ TGS1-like influences ovule, gametophyte and possibly embryo development. 2024-10-31T15:44:12Z 2024-10-31T15:44:12Z 2019-11 Artículo Colono, Carolina, et al., 2019. A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules. Frontiers in Plant Science. Carolina del Norte: Universidad de Carolina del Norte, vol. 10, no. 1566, p. 1-14. E-ISSN 1664-462X. DOI: https://doi.org/10.3389/fpls.2019.01566 http://repositorio.unne.edu.ar/handle/123456789/56240 eng https://doi.org/10.3389/fpls.2019.01566 openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ application/pdf p. 1-14 application/pdf Universidad de Carolina del Norte Frontiers in Plant Science, 2019, vol. 10, no. 1566, p. 1-14.
institution Universidad Nacional del Nordeste
institution_str I-48
repository_str R-184
collection RIUNNE - Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
language Inglés
topic Apomixis
Apospory
Methyltransferase
Plant reproduction
TGS1
spellingShingle Apomixis
Apospory
Methyltransferase
Plant reproduction
TGS1
Colono, Carolina
Ortiz, Juan Pablo Amelio
Permingeat, Hugo Raúl
Souza Canada, Eduardo Daniel
Siena, Lorena Adelina
Spoto, Nicolás
Galdeano, Florencia
Espinoza, Francisco
Leblanc, Olivier
Pessino, Silvina Claudia
A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules
topic_facet Apomixis
Apospory
Methyltransferase
Plant reproduction
TGS1
description Aposporous apomictic plants form clonal maternal seeds by inducing the emergence of non-reduced (2n) embryo sacs in the ovule nucellus and the development of embryos by parthenogenesis. In previous work, we reported a plant-specific TRIMETHYLGUANOSINE SYNTHASE 1 (TGS1) gene (PN_TGS1-like) showing expression levels positively correlated with sexuality rates in facultative apomictic Paspalum notatum. PN_ TGS1-like displayed contrasting in situ hybridization patterns in apomictic and sexual plant ovules from premeiosis to anthesis. Here we transformed sexual P. notatum with a TGS1-like antisense construction under a constitutive promoter, in order to produce lines with reduced transcript representation. Antisense plants developed prominent trichomes on the adaxial leaf surface, a trait absent from control genotypes. Reproductive development analysis revealed occasional formation of twin ovules. While control individuals typically displayed a single meiotic embryo sac per ovule, antisense lines showed 12.93–15.79% of ovules bearing extra nuclei, which can be assigned to aposporous-like embryo sacs (AES-like) or, alternatively, to gametophytes with a misguided cell fate development. Moreover, around 8.42–9.52% of ovules showed what looked like a combination of meiotic and aposporous-like sacs. Besides, 32.5% of ovules at early developmental stages displayed nucellar cells with prominent nuclei resembling apospory initials (AIs), which surrounded the megaspore mother cell (MMC) or the MMC-derived meiotic products. Two or more concurrent meiosis events were never detected, which suggest a non-reduced nature for the extra nuclei observed in the mature ovules, unless they were generated by proliferation and misguided differentiation of the legitimate meiotic products. The antisense lines produced a similar amount of viable even-sized pollen with respect to control genotypes, and formed an equivalent full seed set (~9% of total seeds) after selfpollination. Flow cytometry analyses of caryopses derived from antisense lines revealed that all full seeds had originated from meiotic embryo sacs (i.e. by sexuality). A reduction of 25.55% in the germination percentage was detected when comparing antisense lines with controls. Our results indicate that PN_ TGS1-like influences ovule, gametophyte and possibly embryo development.
format Artículo
author Colono, Carolina
Ortiz, Juan Pablo Amelio
Permingeat, Hugo Raúl
Souza Canada, Eduardo Daniel
Siena, Lorena Adelina
Spoto, Nicolás
Galdeano, Florencia
Espinoza, Francisco
Leblanc, Olivier
Pessino, Silvina Claudia
author_facet Colono, Carolina
Ortiz, Juan Pablo Amelio
Permingeat, Hugo Raúl
Souza Canada, Eduardo Daniel
Siena, Lorena Adelina
Spoto, Nicolás
Galdeano, Florencia
Espinoza, Francisco
Leblanc, Olivier
Pessino, Silvina Claudia
author_sort Colono, Carolina
title A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules
title_short A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules
title_full A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules
title_fullStr A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules
title_full_unstemmed A plant-specific TGS1 homolog influences gametophyte development in sexual tetraploid Paspalum notatum ovules
title_sort plant-specific tgs1 homolog influences gametophyte development in sexual tetraploid paspalum notatum ovules
publisher Universidad de Carolina del Norte
publishDate 2024
url http://repositorio.unne.edu.ar/handle/123456789/56240
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