Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration
The aim of the present study was to investigate the efficiency of the encapsulation-dehydra- tion technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120...
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| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Tech Science Press
2024
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| Materias: | |
| Acceso en línea: | http://repositorio.unne.edu.ar/handle/123456789/55354 |
| Aporte de: |
| Sumario: | The aim of the present study was to investigate the efficiency of the encapsulation-dehydra-
tion technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species
were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after
pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented
with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M
sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid
nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid
mediums [0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)] and
cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal.
Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this
work, the encapsulation-dehydration technique was employed for first time in Cyrtopodium hatschbachii. |
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